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Phospho-LCK (Tyr505) Antibody (44-850G)
Lck Protein Schematic-Lck is a 56 kDa, membrane-associated, non-receptor tyrosine kinase belonging to the Src family that plays a critical role in T-cell antigen receptor (TCR) signaling, inducing T-lymphocyte development, activation, proliferation and survival. Lck has been implicated in various diseases including cancer development and metastasis, viral infections relating to HIV, heart disease, and osteoclast-mediated bone resorption. Lck is regulated by phosphorylation on multiple residues including Ser-194 in the SH2 domain, Tyr-394 (... View More
Product Details
44-850G
Applications
Tested Dilution
Publications
Product Specifications
Published species
Not Applicable
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic phosphopeptide from human Lck containing tyrosine 505 (based on Swiss Protein database, accession number P06239). The sequence is conserved in mouse.
Conjugate
Form
Liquid
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2533779
Target Information
LCK is a member of the Src-family tyrosine kinase, which are essential for signaling through the T-cell receptor (TCR) complex. Upon TCR triggering, LCK phosphorylates the ITAM motives in its zeta subunits establishing binding sites for the SH2 domains of the tyrosine kinase ZAP70, which is also phosphorylated by LCK and activated to generate subsequent signaling platforms by phosphorylation of adaptor LAT. The majority of LCK is localized to the plasma membrane, however, there is also a significant fraction associated with the Golgi apparatus which may contribute to Raf activation under conditions of weak stimulation through the TCR. LCK contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. LCK is also involved in the regulation of apoptosis induced by various stimuli, but not by the death receptors. Diseases associated with LCK include Immunodeficiency 22 and CD45 deficiency. Alternatively splice variants of the LCK gene encoding different isoforms of the protein have been found.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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