Invitrogen

Phospho-p38 MAPK (Thr180, Tyr182) Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

9 Published Figures

34 References

View all (67) p38 MAPK alpha antibodies

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Datasheet

Protocols

Questions & Answers

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Phospho-p38 MAPK (Thr180, Tyr182) Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 20

Phospho-p38 MAPK (Thr180, Tyr182) Antibody (44-684G) in ICC/IF

Immunofluorescent analysis of Phospho-P38 pThr180/pTyr182 Antibody was done on 70% confluent log phase SHSY5Y cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Phospho-P38 pThr180/pTyr182 Antibody (Product # 44-684G) at 1µg/mL in 1% BSA and incubated f... View More

Phospho-p38 MAPK (Thr180, Tyr182) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-p38 MAPK (Thr180, Tyr182) Antibody in Western Blot (WB)

Phospho-p38 MAPK (Thr180, Tyr182) Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Phospho-p38 MAPK (Thr180, Tyr182) Antibody

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Product Details

44-684G

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 29 publications 29 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1 µg/mL

View 1 publication 1 publication

Flow Cytometry (Flow)

3-5 µg/1x10^6 cells

Immunoprecipitation (IP)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human p38 that contains threonine 180 and tyrosine 182. This region is conserved among many species including mouse, rat, dog, monkey and carp.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533721

Product Specific Information

This antibody reacts with human and rat (100% homologous) p38 alpha, beta, and gamma. HEK 293 cells +/- UV irradiation treatment and PC12 cells +/- Sorbitol were used as positive controls.

Purified from rabbit serum by sequential epitope-specific chromatography, this product contains enough material for 10 mini-blots. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated p38. The final product is generated by affinity chromatography using a p38-derived peptide that is phosphorylated at threonine 180 and tyrosine 182.

Target Information

MAPK14 (p38 alpha) is a member of the stress-activated protein kinase class of MAPKs. The p38 MAPK cascade regulates a variety of cellular responses to stress, inflammation, and other signals. p38 MAPK is relatively inactive in the nonphosphorylated form and becomes rapidly activated by dual phosphorylation of a Thr-Gly-Tyr motifs. There are four isoforms of p38 MAPK, which differ in their tissue expression and affinity for upstream activators and downstream effectors. When cells are exposed to tumor necrosis factor, interleukin-1, heat shock, or other activating stimuli, activation of MAPK kinase-3 occurs by phosphorylation. Activated MAPK kinase-3/6 phosphorylate each residue of Thr180 and Tyr182 in p38 MAPK. Phospho-p38 MAPK activates ATF-2, CHOP-1, MEF-2 and other transcription factors through phosphorylation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Electroacupuncture Attenuates Fibromyalgia Pain via Toll-like Receptor 4 in the Mouse Brain.

Life (Basel, Switzerland)

Lai PC,Yen CM,Lin MC,Chen YH,Liao HY,Huang YW,Lin YW

44-684G was used in Western Blotting to show that inflammation can activate the TLR4 pathway and provided new possible therapeutic targets for FM pain.

Thu May 11 00:00:00 EDT 2023

EphA3 targeted by miR-3666 contributes to melanoma malignancy via activating ERK1/2 and p38 MAPK pathways.

Open medicine (Warsaw, Poland)

Ming D,Ma J

44-684G was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to conclude that miR-3666 downregulated EphA3 expression and retarded melanoma malignancy via inactivating ERK1/2 and p38 MAPK pathways.

Tue Jan 03 00:00:00 EST 2023

CoQ10 augments candesartan protective effect against tourniquet-induced hind limb ischemia-reperfusion: Involvement of non-classical RAS and ROS pathways.

Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society

Awad AS,Nour El-Din M,Kamel R

44-684G was used in Western Blotting to show the impact of CoQ10 on skeletal muscle renin-angiotensin system in rats.

Thu Jul 01 00:00:00 EDT 2021

CoQ10 augments candesartan protective effect against tourniquet-induced hind limb ischemia-reperfusion: Involvement of non-classical RAS and ROS pathways.

Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society

Awad AS,Nour El-Din M,Kamel R

44-684G was used in Western Blotting to show the impact of CoQ10 on skeletal muscle renin-angiotensin system in rats.

Thu Jul 01 00:00:00 EDT 2021

Downregulation of FPR1 abates lipopolysaccharide-induced inflammatory injury and apoptosis by upregulating MAPK signaling pathway in murine chondrogenic ATDC5 cells.

Allergologia et immunopathologia

Chen H,Zhang L

44-684G was used in Western Blot to reveal that FPR1 was highly expressed in LPS-induced chondrocytes ATDC5, and the downregulation of FPR1 abated the inflammatory response and apoptosis of LPS-ATDC5 cells by regulating the MAPK signaling pathway.

Wed Feb 09 00:00:00 EST 2022

Lotus leaf flavonoids induce apoptosis of human lung cancer A549 cells through the ROS/p38 MAPK pathway.

Biological research

Jia XB,Zhang Q,Xu L,Yao WJ,Wei L

44-684G was used in Western Blotting to verify the effect of LLF on apoptosis in these cells through the ROS/p38 MAPK pathway.

Tue Mar 02 00:00:00 EST 2021

Evidence for acupoint catgut embedding treatment and TRPV1 gene deletion increasing weight control in murine model.

International journal of molecular medicine

Inprasit C,Huang YC,Lin YW

44-684G was used in Western Blotting to show body weight maintenance may be modulated by calcium ion channel TRPV1.

Sun Mar 01 00:00:00 EST 2020

Evidence for acupoint catgut embedding treatment and TRPV1 gene deletion increasing weight control in murine model.

International journal of molecular medicine

Inprasit C,Huang YC,Lin YW

44-684G was used in Western Blotting to show body weight maintenance may be modulated by calcium ion channel TRPV1.

Sun Mar 01 00:00:00 EST 2020

A novel protective role of sacubitril/valsartan in cyclophosphamide induced lung injury in rats: impact of miRNA-150-3p on NF-κB/MAPK signaling trajectories.

Scientific reports

Abdel-Latif GA,Elwahab AHA,Hasan RA,ElMongy NF,Ramzy MM,Louka ML,Schaalan MF

44-684G was used in Western Blotting to conclude that sacubitril/valsartan effectively abrogated the CP induced lung inflammation and fibrosis, providing a potential promising protection that could be linked to their ability to inhibit miR-150-3p via inhibition of NF-κB and MAPK signaling pathways.

Mon Aug 03 00:00:00 EDT 2020

A novel protective role of sacubitril/valsartan in cyclophosphamide induced lung injury in rats: impact of miRNA-150-3p on NF-κB/MAPK signaling trajectories.

Scientific reports

Abdel-Latif GA,Elwahab AHA,Hasan RA,ElMongy NF,Ramzy MM,Louka ML,Schaalan MF

Mon Aug 03 00:00:00 EDT 2020

Hypo-phosphorylated CD147 promotes migration and invasion of hepatocellular carcinoma cells and predicts a poor prognosis.

Cellular oncology (Dordrecht)

Jin J,Wang SJ,Cui J,Li L,Li JY,Liu FL,Sun XX,Jiang JL,Cui HY,Chen ZN

44-684G was used in Western Blotting to determine the role of deregulation of CD147 phosphorylation during cancer progression.

Thu Aug 01 00:00:00 EDT 2019

Whole Genome Microarray Analysis of DUSP4-Deletion Reveals A Novel Role for MAP Kinase Phosphatase-2 (MKP-2) in Macrophage Gene Expression and Function.

International journal of molecular sciences

Neamatallah T,Jabbar S,Tate R,Schroeder J,Shweash M,Alexander J,Plevin R

Published figure using Phospho-p38 MAPK (Thr180, Tyr182) polyclonal antibody (Product # 44-684G) in Western Blot

Fri Jul 12 00:00:00 EDT 2019

12-Lipoxygenase Inhibitor Improves Functions of Cytokine-Treated Human Islets and Type 2 Diabetic Islets.

The Journal of clinical endocrinology and metabolism

Ma K,Xiao A,Park SH,Glenn L,Jackson L,Barot T,Weaver JR,Taylor-Fishwick DA,Luci DK,Maloney DJ,Mirmira RG,Imai Y,Nadler JL

44-684G was used in Western Blotting to test the efficacy of ML355, a highly selective, small molecule inhibitor of 12-lipoxygenase, for the preservation of islet function.

Tue Aug 01 00:00:00 EDT 2017

RARβ2-dependent signaling represses neuronal differentiation in mouse ES cells.

Differentiation; research in biological diversity

Kona SL,Shrestha A,Yi X,Joseph S,Barona HM,Martinez-Ceballos E

44-684G was used in Western Blotting to study the role played by specific retinoic acid receptor types on embryonic stem cell differentiation.

Wed Aug 08 00:00:00 EDT 2018

Exosomal miR-141-3p regulates osteoblast activity to promote the osteoblastic metastasis of prostate cancer.

Oncotarget

Ye Y,Li SL,Ma YY,Diao YJ,Yang L,Su MQ,Li Z,Ji Y,Wang J,Lei L,Fan WX,Li LX,Xu Y,Hao XK

44-684G was used in Western Blotting to discover that exosomal miR-141-3p from MDA prostate cancer 2b cells promoted osteoblast activity and regulated the microenvironment of bone metastases.

Tue Nov 07 00:00:00 EST 2017

Cathepsin S attenuates endosomal EGFR signalling: A mechanical rationale for the combination of cathepsin S and EGFR tyrosine kinase inhibitors.

Scientific reports

Huang CC,Lee CC,Lin HH,Chang JY

44-684G was used in western blot to characterize attenuation of endosomal EGFR signaling by cathepsin S as a mechanical rationale for the combination of EGFR tyrosine kinase and cathepsin S inhibitors

Fri Jul 08 00:00:00 EDT 2016

The genomic landscape of diffuse intrinsic pontine glioma and pediatric non-brainstem high-grade glioma.

Nature genetics

Wu G,Diaz AK,Paugh BS,Rankin SL,Ju B,Li Y,Zhu X,Qu C,Chen X,Zhang J,Easton J,Edmonson M,Ma X,Lu C,Nagahawatte P,Hedlund E,Rusch M,Pounds S,Lin T,Onar-Thomas A,Huether R,Kriwacki R,Parker M,Gupta P,Becksfort J,Wei L,Mulder HL,Boggs K,Vadodaria B,Yergeau D,Russell JC,Ochoa K,Fulton RS,Fulton LL,Jones C,Boop FA,Broniscer A,Wetmore C,Gajjar A,Ding L,Mardis ER,Wilson RK,Taylor MR,Downing JR,Ellison DW,Zhang J,Baker SJ

44-684G was used in western blot to identify signaling pathways that contribute to pediatric high-grade glioma

Thu May 01 00:00:00 EDT 2014

MAPK14/p38α-dependent modulation of glucose metabolism affects ROS levels and autophagy during starvation.

Autophagy

Desideri E,Vegliante R,Cardaci S,Nepravishta R,Paci M,Ciriolo MR

44-684G was used in immunocytochemistry and western blot to investigate MAPK14-driven metabolic reprogramming

Mon Sep 01 00:00:00 EDT 2014

MAPK14/p38α-dependent modulation of glucose metabolism affects ROS levels and autophagy during starvation.

Autophagy

Desideri E,Vegliante R,Cardaci S,Nepravishta R,Paci M,Ciriolo MR

44-684G was used in immunocytochemistry and western blot to investigate MAPK14-driven metabolic reprogramming

Mon Sep 01 00:00:00 EDT 2014

Expansion of oligodendrocyte progenitor cells following SIRT1 inactivation in the adult brain.

Nature cell biology

Rafalski VA,Ho PP,Brett JO,Ucar D,Dugas JC,Pollina EA,Chow LM,Ibrahim A,Baker SJ,Barres BA,Steinman L,Brunet A

44-684G was used in western blot to elucidate factors that regulate the generation of adult oligodendrocytes.

Sat Jun 01 00:00:00 EDT 2013

Molecular pathway profiling of T lymphocyte signal transduction pathways; Th1 and Th2 genomic fingerprints are defined by TCR and CD28-mediated signaling.

BMC immunology

Smeets RL,Fleuren WW,He X,Vink PM,Wijnands F,Gorecka M,Klop H,Bauerschmidt S,Garritsen A,Koenen HJ,Joosten I,Boots AM,Alkema W

Wed Mar 14 00:00:00 EDT 2012

T-cell receptor ligation induces distinct signaling pathways in naive vs. antigen-experienced T cells.

Proceedings of the National Academy of Sciences of the United States of America

Adachi K,Davis MM

Tue Jan 25 00:00:00 EST 2011

T-cell receptor ligation induces distinct signaling pathways in naive vs. antigen-experienced T cells.

Proceedings of the National Academy of Sciences of the United States of America

Adachi K,Davis MM

Tue Jan 25 00:00:00 EST 2011

Growth-factor receptor-bound protein-2 (Grb2) signaling in B cells controls lymphoid follicle organization and germinal center reaction.

Proceedings of the National Academy of Sciences of the United States of America

Jang IK,Cronshaw DG,Xie LK,Fang G,Zhang J,Oh H,Fu YX,Gu H,Zou Y

Tue May 10 00:00:00 EDT 2011

Differential modulation of TLR3- and TLR4-mediated dendritic cell maturation and function by progesterone.

Journal of immunology (Baltimore, Md. : 1950)

Jones LA,Kreem S,Shweash M,Paul A,Alexander J,Roberts CW

44-684G was used in western blot to study how progesterone and TLR agonists affect dendritic cell function

Fri Oct 15 00:00:00 EDT 2010

TGFbeta1 antagonistic peptides inhibit TGFbeta1-dependent angiogenesis.

Biochemical pharmacology

Serratì S,Margheri F,Pucci M,Cantelmo AR,Cammarota R,Dotor J,Borràs-Cuesta F,Fibbi G,Albini A,Del Rosso M

44-684G was used in western blot to investigate the cancer blocking activity of TGFbeta antagonist peptides

Sun Mar 01 00:00:00 EST 2009

Critical role of c-jun (NH2) terminal kinase in paracetamol- induced acute liver failure.

Gut

Henderson NC,Pollock KJ,Frew J,Mackinnon AC,Flavell RA,Davis RJ,Sethi T,Simpson KJ

Sun Jul 01 00:00:00 EDT 2007

Critical role of c-jun (NH2) terminal kinase in paracetamol- induced acute liver failure.

Gut

Henderson NC,Pollock KJ,Frew J,Mackinnon AC,Flavell RA,Davis RJ,Sethi T,Simpson KJ

Sun Jul 01 00:00:00 EDT 2007

Chemotherapeutic agents up-regulate the cytomegalovirus promoter: implications for bioluminescence imaging of tumor response to therapy.

Cancer research

Svensson RU,Barnes JM,Rokhlin OW,Cohen MB,Henry MD

44-684G was used in western blot to study the promoter-dependent increase in bioluminescence intensity from CMV-driven luciferase

Thu Nov 01 00:00:00 EDT 2007

Molecular mechanisms involved in interleukin-4-induced human neutrophils: expression and regulation of suppressor of cytokine signaling.

Journal of leukocyte biology

Ratthé C,Pelletier M,Chiasson S,Girard D

Tue May 01 00:00:00 EDT 2007

The peroxisome proliferator-activated receptor gamma agonist pioglitazone reduces the development of cartilage lesions in an experimental dog model of osteoarthritis: in vivo protective effects mediated through the inhibition of key signaling and catabolic pathways.

Arthritis and rheumatism

Boileau C,Martel-Pelletier J,Fahmi H,Mineau F,Boily M,Pelletier JP

44-684G was used in immunohistochemistry - paraffin section to assess the effects of pioglitazone treatment on the development of lesions in a canine model of osteoarthritis

Sun Jul 01 00:00:00 EDT 2007

The mitogen-activated protein kinases (MAPK) p38 and JNK are markers of tumor progression in breast carcinoma.

Gynecologic oncology

Davidson B,Konstantinovsky S,Kleinberg L,Nguyen MT,Bassarova A,Kvalheim G,Nesland JM,Reich R

Fri Sep 01 00:00:00 EDT 2006

Protein synthesis persists during necrotic cell death.

The Journal of cell biology

Saelens X,Festjens N,Parthoens E,Vanoverberghe I,Kalai M,van Kuppeveld F,Vandenabeele P

Mon Feb 14 00:00:00 EST 2005

TNF-alpha promotes a stop signal that inhibits neutrophil polarization and migration via a p38 MAPK pathway.

Journal of leukocyte biology

Lokuta MA,Huttenlocher A

Fri Jul 01 00:00:00 EDT 2005

Urokinase-induced smooth muscle cell responses require distinct signaling pathways: a role for the epidermal growth factor receptor.

Journal of vascular surgery

Nicholl SM,Roztocil E,Davies MG

Fri Apr 01 00:00:00 EST 2005

Plasmin induces smooth muscle cell proliferation.

The Journal of surgical research

Nicholl SM,Roztocil E,Galaria II,Davies MG

Fri Jul 01 00:00:00 EDT 2005

Chemokine receptor CCR2 expression by systemic sclerosis fibroblasts: evidence for autocrine regulation of myofibroblast differentiation.

Arthritis and rheumatism

Carulli MT,Ong VH,Ponticos M,Shiwen X,Abraham DJ,Black CM,Denton CP

44-684G was used in western blot to assess the contribution of CCL2/CCR2 signaling to systemic sclerosis

Thu Dec 01 00:00:00 EST 2005

Bioinformatics

Protein Aliases: CSAID-binding protein; Csaids binding protein; cytokine suppressive anti-inflammatory drug binding protein; Cytokine suppressive anti-inflammatory drug-binding protein; cytokine-supressive anti-inflammatory drug binding protein; EC 2.7.11.24; ERK1; MAP 14; MAP kinase 14; MAP kinase 2; MAP kinase MXI2; MAP kinase p38; MAP kinase p38 alpha; MAP kinase p38alpha; MAPK 14; MAPK1 antibody; MAX-interacting protein 2; Mitogen-activated protein kinase 14; mitogen-activated protein kinase 14A; Mitogen-activated protein kinase p38 alpha; MK14; OTTHUMP00000216563; OTTHUMP00000216566; p38 MAP kinase; p38 mitogen activated protein kinase; p38alpha Exip; reactive kinase; RP1-179N16.5; SAPK2a; Stress-activated protein kinase 2a

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Gene Aliases: CRK1; CSBP; CSBP1; CSBP2; CSPB1; EXIP; Hog; MAPK14; MXI2; p38; p38ALPHA; p38Hog; PRKM14; PRKM15; RK; SAPK2A

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UniProt ID: (Human) Q16539

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Entrez Gene ID: (Human) 1432, (Rat) 81649

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Function(s)

protein serine/threonine kinase activity MAP kinase activity MAP kinase kinase activity protein binding ATP binding protein C-terminus binding enzyme binding protein phosphatase binding NFAT protein binding protein kinase activity kinase activity non-receptor serine/threonine protein kinase

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Phospho-p38 MAPK (Thr180, Tyr182) Polyclonal Antibody

Phospho-p38 MAPK (Thr180, Tyr182) Antibody (44-684G) in ICC/IF

Product Details

44-684G

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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