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SHC Antibody (44829M) in WB
Whole cell lysates of serum starved HeLa (1), HepG2 (2), HEK293 (3), SH-SY5Y (4), MDCK (5) PC12 (6), CMT 93 (7), Neuro 2A (8) and 3T3 (9) tumor cells (approximately 20,000 cells per lane) were resolved by SDS-PAGE and transferred to PVDF. The membrane was blocked with a casein/Tween 20 buffer, then incubated with mouse anti-Shc/p66 (N-term) antibody (Catalog no. 44829M) at 0.5 µg/mL for 1 hour at room temperature. After washing, the membrane was incubated with an anti-mouse HRP-conjugated secondary antibody and signals were detected using ... View More
Product Details
44829M
Product Specifications
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
24E4
Immunogen
Peptide (N-terminal sequence) conjugated to hemocyanin.
Conjugate
Form
Liquid
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Dry ice
RRID
AB_2533773
Target Information
Skp2 (S-phase Kinase-associated Protein 2) belongs to the family of F-box proteins that interact with the Cyclin A-Cdk2 complex. Skp2 is essential for the G1-S transition in both transformed cells and diploid fibroblasts. Biochemical and genetic experiments have demonstrated that Skp2 is required for the ubiquitination and consequent degradation of p27 in cultured mammalian cells and in vitro reconstitution assays. In normal tissues, Skp2 is expressed in tonsil and placenta. In tumor tissues, Skp2 is expressed widely, in some colon, prostate, pancreas, and skin cancers, with high expression in lung, breast, ovarian, and endometrial cancers. Research suggests that Skp2 plays a role in oncogenesis, particularly in the pathogenesis of lymphomas and breast cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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