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Invitrogen
Phospho-STAT4 (Tyr693) Polyclonal Antibody
FIGURE: 1 / 5
Phospho-STAT4 (Tyr693) Antibody (71-7900) in ICC/IF
Immunofluorescence analysis of STAT4 (pY693) was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with STAT4 (pY693) Rabbit polyclonal Antibody (Product # 71-7900) at 2 µg/mL in 1% BSA and incubated for 3 hours at room ... View More
Product Details
71-7900
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse,
Virus
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic tyrosine phosphorylated peptide encompassing and phosphorylated at the conserved tyrosine phosphorylation site (Y-693) of the murine STAT4 protein.
Conjugate
Form
Liquid
Concentration
0.25 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2533998
Target Information
STAT4 was originally identified using degenerate primers complementary to sequences encoding conserved regions of other STAT proteins. The STAT4 protein is most similar to STAT1 (52%) and STAT3 (47%). Functionally, STAT4 is similar to other STAT family members in that it can be tyrosine phosphorylated by Jak1 or Jak2. STAT4 forms homodimers and heterodimers with related STAT family members. Tyrosine phosphorylated STAT4 can bind the IFN-gamma activated site (GAS). Serine phosphorylation of STAT is also required for maximal transcriptional activity. STAT4 expression is restricted to the thymus, spleen and testis. Until recently the cytokine(s) responsible for activation of STAT4 had not been identified. STAT4 is now known to be activated by the cytokine interleukin 12 (IL-12). IL-12 is required for the T-cell independent induction of IFN-gamma which is a key step in the initial suppression of bacterial and parasitic infections. In addition, IL-12 is required for the development of a Th1 response which is necessary for effective host defense against intracellular pathogens. STAT4-deficient mice display impaired IL-12 development of Th1 cells and enhanced development of Th2 cells. A recent study in mouse has shown that in response to viral infection IFN-a/b activation of STAT4 is required for IFN-g production.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CIS4; HSPC060; OTTHUMP00000163553; Signal transducer and activator of transcription 4; SOCS4; SOCS6; SSI4; STAI4; STAT; STATI4
Gene Aliases: SLEB11; STAT4
UniProt ID: (Human) Q14765, (Mouse) P42228
Entrez Gene ID: (Human) 6775, (Mouse) 20849
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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