Invitrogen

Phospho-TAK1 (Thr184, Thr187) Monoclonal Antibody (K.846.3)

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Antibody Testing Data (1)
Published Figures (2)

Phospho-TAK1 (Thr184, Thr187) Antibody in Western Blot (WB)

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Phospho-TAK1 (Thr184, Thr187) Antibody (MA5-15073) in WB

Western blot analysis of Phospho-TAK1 pThr184/187 in extracts from 293 IL-1R cells, untreated or treated for 10 minutes with IL-1, using Phospho-TAK1 pThr184/187 monoclonal antibody (Product # MA5-15073) (upper) or a TAK1 polyclonal antibody (lower).

Phospho-TAK1 (Thr184, Thr187) Antibody in Western Blot, Immunohistochemistry (Frozen) (WB, IHC (F))

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Product Details

MA5-15073

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 3 publications 3 publications

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

K.846.3

Immunogen

Phosphopeptide corresponding to residues surrounding pThr184 and pThr187 of human TAK1

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

24 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_10982333

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

TAK1 (also known as MAP3K7), a part of the serine/threonine kinase family, is a versatile protein with many signaling functions. Originally identified as a TGF- beta-activated kinase, TAK1 interacts with TGF- beta-receptor and TRAF6 to modulate TGF- beta activation of JNK and p38. It has additional roles in activation of p38 and JNK through Wnt, BMP, and activin signaling pathways as well in response to thyroid hormone, osmotic stress, endothelin, and ephrine. Through activation of von Hippel-Lindau tumor suppressor expression, TAK1 represses PDGF-B, integrin beta1 and integrin beta5, promoting proper wound healing. TAK1 also plays an essential role in IKK activation in numerous signaling pathways including IL-1, IL-6, IL-18, TNF, CD40, TLR, and RIG-I. Activation of the TAK1-IKK pathway requires TAB2/3 and ubiquitination. In this process, TAB2/3 binds to the C-terminal region of TAK1 and becomes polyubiquitinated, TAK1 is autophosphorylated at Thr178, Thr184, Thr187 and Ser192, and finally TAK1 phosphorylates IKK- beta. Additionally, TAK1 represses human telomerase reverse transcriptase suggesting a role in regulation of cell lifespan.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Albumin inhibits the nuclear translocation of Smad3 via interleukin-1beta signaling in hepatic stellate cells.

Scientific reports

Park JH,Kim J,Choi SY,Lee B,Lee JE,Park H,Moon JW,Park SH,Lee JM,Lee HS,Oh J

MA5-15073 was used in Western Blot to elucidate the mechanism of action of albumin downstream of RA sequestration.

Thu Feb 04 00:00:00 EST 2021

Albumin inhibits the nuclear translocation of Smad3 via interleukin-1beta signaling in hepatic stellate cells.

Scientific reports

Park JH,Kim J,Choi SY,Lee B,Lee JE,Park H,Moon JW,Park SH,Lee JM,Lee HS,Oh J

MA5-15073 was used in Western Blot to elucidate the mechanism of action of albumin downstream of RA sequestration.

Thu Feb 04 00:00:00 EST 2021

TAK1 mediates neuronal pyroptosis in early brain injury after subarachnoid hemorrhage.

Journal of neuroinflammation

Xu P,Tao C,Zhu Y,Wang G,Kong L,Li W,Li R,Li J,Zhang C,Wang L,Liu X,Sun W,Hu W

MA5-15073 was used in Immunocytochemistry-immunoflourescence to investigate the effects of transforming growth factor-β-activated kinase 1 on neuroinflammation in early brain injury following subarachnoid hemorrhage are largely unknown.

Mon Aug 30 00:00:00 EDT 2021

TAK1 mediates neuronal pyroptosis in early brain injury after subarachnoid hemorrhage.

Journal of neuroinflammation

Xu P,Tao C,Zhu Y,Wang G,Kong L,Li W,Li R,Li J,Zhang C,Wang L,Liu X,Sun W,Hu W

Mon Aug 30 00:00:00 EDT 2021

TAK1 mediates neuronal pyroptosis in early brain injury after subarachnoid hemorrhage.

Journal of neuroinflammation

Xu P,Tao C,Zhu Y,Wang G,Kong L,Li W,Li R,Li J,Zhang C,Wang L,Liu X,Sun W,Hu W

Mon Aug 30 00:00:00 EDT 2021

Toll-Like Receptor 9-Dependent AMPKα Activation Occurs via TAK1 and Contributes to RhoA/ROCK Signaling and Actin Polymerization in Vascular Smooth Muscle Cells.

The Journal of pharmacology and experimental therapeutics

McCarthy CG,Wenceslau CF,Ogbi S,Szasz T,Webb RC

MA5-15073 was used in Western Blotting to investigate if noncanonical toll-like receptor 9 (TLR9) signaling is present in vascular smooth muscle cells (VSMCs) and if it mediates enhanced contractile responses through sarcoplasmic/endoplasmic reticulum Ca2+-ATPase 2 (SERCA2) inhibition.

Sun Apr 01 00:00:00 EDT 2018

TAK1 modulates satellite stem cell homeostasis and skeletal muscle repair.

Nature communications

Ogura Y,Hindi SM,Sato S,Xiong G,Akira S,Kumar A

MA5-15073 was used in flow cytometry to characterize TAK1 and modulation of skeletal muscle repair and satelline stem cell homeostasis

Wed Dec 09 00:00:00 EST 2015

Bioinformatics

Protein Aliases: M3K7; Mitogen-activated protein kinase kinase kinase 7; RP1-154G14.1; TGF-beta activated kinase 1; TGF-beta-activated kinase 1; Transforming growth factor-beta-activated kinase 1

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Gene Aliases: MAP3K7; MEKK7; TAK1; TGF1a

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UniProt ID: (Human) O43318

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Entrez Gene ID: (Human) 6885

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Function(s)

magnesium ion binding protein kinase activity protein serine/threonine kinase activity MAP kinase kinase kinase activity protein binding ATP binding scaffold protein binding non-receptor serine/threonine protein kinase protein modifying enzyme

Process(es)

activation of MAPKK activity activation of MAPK activity stimulatory C-type lectin receptor signaling pathway positive regulation of T cell cytokine production MyD88-dependent toll-like receptor signaling pathway transcription, DNA-templated apoptotic process transforming growth factor beta receptor signaling pathway Wnt signaling pathway, calcium modulating pathway I-kappaB kinase/NF-kappaB signaling activation of NF-kappaB-inducing kinase activity I-kappaB phosphorylation JNK cascade positive regulation of interleukin-2 production Fc-epsilon receptor signaling pathway positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of JUN kinase activity histone H3 acetylation T cell receptor signaling pathway positive regulation of T cell activation positive regulation of NF-kappaB transcription factor activity stress-activated MAPK cascade nucleotide-binding oligomerization domain containing signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Phospho-TAK1 (Thr184, Thr187) Monoclonal Antibody (K.846.3)

Phospho-TAK1 (Thr184, Thr187) Antibody (MA5-15073) in WB

Product Details

MA5-15073

Western Blot (WB)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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