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Product Details
PA5-20811
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A 15 amino acid peptide from near the amino terminus of human RIPK1.
Conjugate
Form
Liquid
Concentration
1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_11154790
Product Specific Information
A suggested positive control is rat kidney tissue lysate.
PA5-20811 can be used with blocking peptide PEP-0925.
Target Information
Serine-threonine kinase which transduces inflammatory and cell-death signals (programmed necrosis) following death receptors ligation, activation of pathogen recognition receptors (PRRs), and DNA damage. Upon activation of TNFR1 by the TNF-alpha family cytokines, TRADD and TRAF2 are recruited to the receptor. Phosphorylates DAB2IP at 'Ser-728' in a TNF-alpha-dependent manner, and thereby activates the MAP3K5-JNK apoptotic cascade. Ubiquitination by TRAF2 via 'Lys-63'-link chains acts as a critical enhancer of communication with downstream signal transducers in the mitogen-activated protein kinase pathway and the NF-kappa-B pathway, which in turn mediate downstream events including the activation of genes encoding inflammatory molecules. Polyubiquitinated protein binds to IKBKG/NEMO, the regulatory subunit of the IKK complex, a critical event for NF-kappa-B activation. Interaction with other cellular RHIM-containing adapters initiates gene activation and cell death. RIPK1 and RIPK3 association, in particular, forms a necrosis-inducing complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Cell death protein RIP; FLJ39204; OTTHUMP00000015955; receptor (TNFRSF)-interacting serine-threonine kinase 1; Receptor-interacting protein 1; receptor-interacting protein kinase 1; Receptor-interacting serine/threonine-protein kinase 1; RIP; RIP-1; RIP1 (CT); serine/threonine-protein kinase RIP
Gene Aliases: D330015H01Rik; Rinp; RIP; RIP-1; RIP1; RIPK1
UniProt ID: (Human) Q13546, (Mouse) Q60855
Entrez Gene ID: (Human) 8737, (Rat) 306886, (Mouse) 19766
protein kinase activity
protein serine/threonine kinase activity
death receptor binding
protein binding
ATP binding
ubiquitin protein ligase binding
protein complex binding
identical protein binding
death domain binding
nucleotide binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
positive regulation of protein phosphorylation
apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic process
I-kappaB kinase/NF-kappaB signaling
activation of JUN kinase activity
regulation of tumor necrosis factor-mediated signaling pathway
regulation of necrotic cell death
positive regulation of necrotic cell death
positive regulation of interleukin-8 production
positive regulation of tumor necrosis factor production
tumor necrosis factor-mediated signaling pathway
response to tumor necrosis factor
TRIF-dependent toll-like receptor signaling pathway
peptidyl-serine autophosphorylation
positive regulation of apoptotic process
positive regulation of programmed cell death
positive regulation of I-kappaB kinase/NF-kappaB signaling
negative regulation of I-kappaB kinase/NF-kappaB signaling
cellular protein catabolic process
positive regulation of macrophage differentiation
positive regulation of transcription from RNA polymerase II promoter
positive regulation of JNK cascade
protein autophosphorylation
positive regulation of NF-kappaB transcription factor activity
protein homooligomerization
protein heterooligomerization
positive regulation of necroptotic process
T cell apoptotic process
necroptotic process
regulation of ATP:ADP antiporter activity
cellular response to tumor necrosis factor
cellular response to growth factor stimulus
death-inducing signaling complex assembly
apoptotic signaling pathway
extrinsic apoptotic signaling pathway
activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway
ripoptosome assembly
necroptotic signaling pathway
ripoptosome assembly involved in necroptotic process
regulation of extrinsic apoptotic signaling pathway via death domain receptors
negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
positive regulation of hydrogen peroxide-induced cell death
amyloid fibril formation
positive regulation of reactive oxygen species metabolic process
negative regulation of extrinsic apoptotic signaling pathway
positive regulation of extrinsic apoptotic signaling pathway
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
positive regulation of cell death
positive regulation of phosphorylation
positive regulation of MAPK cascade
positive regulation of transferase activity
regulation of reactive oxygen species metabolic process
protein phosphorylation
signal transduction
programmed cell death
viral process
phosphorylation
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