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SOCS1 Antibody (37-4100) in IHC (P)
Immunohistochemistry analysis of SOCS-1 showing staining in the nucleus and weak staining in the cytoplasm of paraffin-embedded human spleen tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol ... View More
Product Details
37-4100
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
4H1
Immunogen
Recombinant protein derived from the N-terminal region of the mouse SOCS-1 (Suppressor of Cytokine Signaling-1, also known as SSI-1) protein.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2533316
Target Information
The Suppressor of cytokine signaling (SOCS) and cytokine-inducible SH2 proteins are a family of intracellular proteins which regulate the immune cell responses to cytokines. SOCS1 acts to suppress dendritic cell (DC) as well as T cell hyperactivation following cytokine signaling by inhibiting JAK tyrosine kinase, a kinase necessary for type I and II cytokine receptors to initiate signaling, by directly binding to the catalytic domain of the kinase. SOCS1 also possesses E3 ubiquitin protein ligase activity that results in the polyubiquitination of its target proteins and subsequent degradation by the proteosome. It is through this method that SOCS1 negatively regulates signaling by Toll-like receptors TLR2 and TLR4 by mediating the degradation of the TLR signaling adaptor protein TIRAP.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: cytokine inducible SH2-containing protein 1; cytokine inducible SH2-containing protein 7; JAB; JAK-binding protein; JAK2-binding protein; SOCS-1; SSI-1; STAT-induced STAT inhibitor 1; Suppressor of cytokine signaling 1
Gene Aliases: Cish1; Cish7; JAB; SOCS-1; Socs1; SSI-1; Ssi1
UniProt ID: (Mouse) O35716
Entrez Gene ID: (Mouse) 12703
regulation of protein phosphorylation
negative regulation of protein kinase activity
JAK-STAT cascade
negative regulation of signal transduction
regulation of activation of JAK2 kinase activity
cytokine-mediated signaling pathway
intracellular signal transduction
regulation of growth
regulation of tyrosine phosphorylation of Stat1 protein
negative regulation of tyrosine phosphorylation of Stat3 protein
negative regulation of catalytic activity
fat cell differentiation
regulation of JAK-STAT cascade
negative regulation of JAK-STAT cascade
negative regulation of insulin receptor signaling pathway
regulation of cytokine secretion
regulation of interferon-gamma-mediated signaling pathway
cellular response to amino acid stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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