Invitrogen

TCR V delta 2 Monoclonal Antibody (15D)

TCR V delta 2 Antibody in Western Blot (WB)

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TCR V delta 2 Antibody (TCR1732) in WB

Western blot analysis of TCR V delta 2 was performed by loading 25 µg of Jurkat (lane 1) and CEM (lane 2) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a TCR V delta 2 monoclonal antibody (Product # TCR1732) at a dilution of 1:10 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Sub... View More

TCR V delta 2 Antibody in Flow Cytometry (Flow)

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Product Details

TCR1732

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Flow Cytometry (Flow)

1-2 µg/test

View 11 publications 11 publications

Immunoprecipitation (IP)

Assay-dependent

T-Cell Activation (TCA)

Assay-dependent

Product Specifications

Species Reactivity

Human

Published species

Fruit fly, Human, Non-human primate, Rhesus monkey

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

15D

Immunogen

Human TCR V delta 2

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.15 mg/mL

Purification

Protein A

Storage buffer

PBS with 0.5% BSA

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_417090

Product Specific Information

TCR1732 targets TCR V delta 2 in FACS, IHC (F), IP, WB and TCA applications and shows reactivity with Human samples.

The TCR1732 immunogen is human TCR V delta 2.

Target Information

The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response. TCR recognition of self-peptides has been linked to autoimmune disease. Mutant self-peptides have been associated with tumors. Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome. Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). The autoantibodies block TH1-mediated inflammatory autodestructive reactions and are believed to be a method by which the immune system compensates for disease (ref5). T Cell and TCR Diversity Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP). A small number of T cells lack both CD4 and CD8 (double negative, DN). Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders. Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Phenotypic and functional characterization of pharmacologically expanded Vγ9Vδ2 T cells in pigtail macaques.

iScience

Barber-Axthelm IM,Wragg KM,Esterbauer R,Amarasena TH,Barber-Axthelm VRB,Wheatley AK,Gibbon AM,Kent SJ,Juno JA

TCR1732 was used in Flow Cytometry to show the tissue distribution and phenotype of in vivo pharmacologically expanded Vγ9Vδ2 T cells can be altered based on the antigen administration route, with implications for tissue trafficking and the clinical efficacy of Vγ9Vδ2 T cell immunotherapeutic.

Fri Mar 17 00:00:00 EDT 2023

γδ T Cells Contribute to Injury in the Developing Brain.

The American journal of pathology

Albertsson AM,Zhang X,Vontell R,Bi D,Bronson RT,Supramaniam V,Baburamani AA,Hua S,Nazmi A,Cardell S,Zhu C,Cantor H,Mallard C,Hagberg H,Leavenworth JW,Wang X

TCR1732 was used in Immunohistochemistry to highlight unique features of injury in the developing brain, where, unlike injury in the mature brain, γδT cells function as initiators of injury independently of common γδT-cell-associated cytokines.

Thu Mar 01 00:00:00 EST 2018

HMBPP-deficient Listeria mutant immunization alters pulmonary/systemic responses, effector functions, and memory polarization of Vγ2Vδ2 T cells.

Journal of leukocyte biology

Frencher JT,Shen H,Yan L,Wilson JO,Freitag NE,Rizzo AN,Chen CY,Chen ZW

TCR1732 was used in flow cytometry to examine the effect of HMBPP-deficient Listeria mutant immunization

Mon Dec 01 00:00:00 EST 2014

Characterization of γδT cells in naïve and HIV-infected chimpanzees and their responses to T-cell activators in vitro.

Journal of medical primatology

Hodara VL,Parodi LM,Chavez D,Smith LM,Lanford R,Giavedoni LD

TCR1732 was used in flow cytometry to study the in vitro response of gammadelta T-cell subsets from HIV1-infected and control chimpanzees to T-cell activators

Fri Aug 01 00:00:00 EDT 2014

Phosphoantigen/IL2 expansion and differentiation of Vγ2Vδ2 T cells increase resistance to tuberculosis in nonhuman primates.

PLoS pathogens

Chen CY,Yao S,Huang D,Wei H,Sicard H,Zeng G,Jomaa H,Larsen MH,Jacobs WR,Wang R,Letvin N,Shen Y,Qiu L,Shen L,Chen ZW

TCR1732 was used in flow cytometry to study the increased Tuberculosis resistance of non-human primates following phoshoantigen/IL-2 mediated expansion and differentiation of VgammaVdelta2 T-cells

Thu Aug 01 00:00:00 EDT 2013

Multieffector-functional immune responses of HMBPP-specific Vγ2Vδ2 T cells in nonhuman primates inoculated with Listeria monocytogenes ΔactA prfA*.

Journal of immunology (Baltimore, Md. : 1950)

Ryan-Payseur B,Frencher J,Shen L,Chen CY,Huang D,Chen ZW

TCR1732 was used in flow cytometry to study multi-effector functional Vgamma2Vdelta2 T cell responses against Listeria monocytogenes in nonhuman primates

Wed Aug 01 00:00:00 EDT 2012

Mechanisms underlying γδ T-cell subset perturbations in SIV-infected Asian rhesus macaques.

Blood

Harris LD,Klatt NR,Vinton C,Briant JA,Tabb B,Ladell K,Lifson J,Estes JD,Price DA,Hirsch VM,Brenchley JM

TCR1732 was used in flow cytometry to investigate T cells in chronically SIVsmE543-infected rhesus macaques

Thu Nov 18 00:00:00 EST 2010

Gammadelta T cell immune manipulation during chronic phase of simian-human immunodeficiency virus infection [corrected] confers immunological benefits.

Journal of immunology (Baltimore, Md. : 1950)

Ali Z,Yan L,Plagman N,Reichenberg A,Hintz M,Jomaa H,Villinger F,Chen ZW

TCR1732 was used in Flow Cytometry to investigate whether a well-defined phosphoantigen-4-hydroxy-3-methyl-but-2-enyl pyrophosphate/IL-2 therapeutic regimen can overcome HIV-mediated immune suppression to massively expand polyfunctional Vgamma2Vdelta2 T cells.

Thu Oct 15 00:00:00 EDT 2009

Definition of APC presentation of phosphoantigen (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate to Vgamma2Vdelta 2 TCR.

Journal of immunology (Baltimore, Md. : 1950)

Wei H,Huang D,Lai X,Chen M,Zhong W,Wang R,Chen ZW

TCR1732 was used in western blot to study the antigen presentation of a specific phosphoantigen

Wed Oct 01 00:00:00 EDT 2008

Human CD1-restricted T cell recognition of lipids from pollens.

The Journal of experimental medicine

Agea E,Russano A,Bistoni O,Mannucci R,Nicoletti I,Corazzi L,Postle AD,De Libero G,Porcelli SA,Spinozzi F

TCR1732 was used in flow cytometry to study the immune response of pollen lipids

Mon Jul 18 00:00:00 EDT 2005

In vivo immunomanipulation of V gamma 9V delta 2 T cells with a synthetic phosphoantigen in a preclinical nonhuman primate model.

Journal of immunology (Baltimore, Md. : 1950)

Sicard H,Ingoure S,Luciani B,Serraz C,Fournié JJ,Bonneville M,Tiollier J,Romagné F

TCR1732 was used in flow cytometry to investigate the effect of a synthetic agonist on the stimulation of Vgamma9Vdelta2 in monkeys

Sat Oct 15 00:00:00 EDT 2005

Drug-induced expansion and differentiation of V gamma 9V delta 2 T cells in vivo: the role of exogenous IL-2.

Journal of immunology (Baltimore, Md. : 1950)

Casetti R,Perretta G,Taglioni A,Mattei M,Colizzi V,Dieli F,D'Offizi G,Malkovsky M,Poccia F

TCR1732 was used in flow cytometry to report the functional characteristics of Vgamma9Vdelta2 T cells from Macaca fascicularis

Mon Aug 01 00:00:00 EDT 2005

Inhibition of adaptive Vgamma2Vdelta2+ T-cell responses during active mycobacterial coinfection of simian immunodeficiency virus SIVmac-infected monkeys.

Journal of virology

Zhou D,Lai X,Shen Y,Sehgal P,Shen L,Simon M,Qiu L,Huang D,Du GZ,Wang Q,Letvin NL,Chen ZW

TCR1732 was used in flow cytometry to investigate the effect of mycobacterial coinfection of simian immunodeficiency virus on adaptive Vgamma2Vdelta2+ T-cell responses

Sat Mar 01 00:00:00 EST 2003

Performance Guarantee

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TCR V delta 2 Monoclonal Antibody (15D)

TCR V delta 2 Antibody (TCR1732) in WB

Product Details

TCR1732

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Flow Cytometry (Flow)

Immunoprecipitation (IP)

T-Cell Activation (TCA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Performance Guarantee

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