Invitrogen

XIAP Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

4 Published Figures

6 References

View all (37) XIAP antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

XIAP Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 13

XIAP Antibody (PA5-29253) in ICC/IF

Immunofluorescence analysis of XIAP was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with anti-XIAP Rabbit Polyclonal Antibody (Product # PA5-29253) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Pro... View More

XIAP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

XIAP Antibody in Immunohistochemistry (IHC)

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XIAP Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. Relative expression of XIAP was observed in mouse lung as compared to mouse brain using XIAP Polyclonal Antibody (Product # PA5-29253) in western blot. {KD}

Product Details

PA5-29253

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 263 and 497 of Human XIAP

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 10% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2546729

Product Specific Information

Recommended positive controls: HeLa, Mouse brain.

Predicted reactivity: Mouse (90%), Rat (88%), Dog (85%), Pig (89%), Rabbit (89%), Bovine (87%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

ATP synthase is extremely conserved through evolution and can be found in plants, fungi, bacteria, and animals. The ATP synthase enzyme is a transmembrane protein responsible for driving the reversible reaction from ADP+ phosphate to ATP. This reaction is accomplished by a flux of protons across the membrane as a result of electron transfer. The ATP synthase protein has two main sections; the F1 ATP-ase (soluble) and the F0 ATP-ase (membrane embedded). The F1 section consists of the alpha, beta, gamma, delta, and epsilon subunits. While the F0 consists of a, b, and c subunits.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Suppression of EGFR/PKC-δ/NF-κB Signaling Associated With Imipramine-Inhibited Progression of Non-Small Cell Lung Cancer.

Frontiers in oncology

Yueh PF,Lee YH,Chiang IT,Chen WT,Lan KL,Chen CH,Hsu FT

Published figure using XIAP polyclonal antibody (Product # PA5-29253) in Immunohistochemistry

Thu Apr 28 00:00:00 EDT 2022

Suppression of EGFR/PKC-δ/NF-κB Signaling Associated With Imipramine-Inhibited Progression of Non-Small Cell Lung Cancer.

Frontiers in oncology

Yueh PF,Lee YH,Chiang IT,Chen WT,Lan KL,Chen CH,Hsu FT

Published figure using XIAP polyclonal antibody (Product # PA5-29253) in Immunohistochemistry

Thu Apr 28 00:00:00 EDT 2022

HtrA4 Protease Promotes Chemotherapeutic-Dependent Cancer Cell Death.

Cells

Wenta T,Rychlowski M,Jarzab M,Lipinska B

Published figure using XIAP polyclonal antibody (Product # PA5-29253) in Western Blot

Fri Sep 20 00:00:00 EDT 2019

Fluoxetine Induces Apoptosis through Extrinsic/Intrinsic Pathways and Inhibits ERK/NF-κB-Modulated Anti-Apoptotic and Invasive Potential in Hepatocellular Carcinoma Cells In Vitro.

International journal of molecular sciences

Chen WT,Hsu FT,Liu YC,Chen CH,Hsu LC,Lin SS

PA5-29253 was used in Western Blotting to verify the effects of fluoxetine on dysregulation of apoptosis and invasive potential in human hepatocellular carcinoma (HCC) SK-Hep1 and Hep3B cells.

Mon Feb 11 00:00:00 EST 2019

Protein Kinase B and Extracellular Signal-Regulated Kinase Inactivation is Associated with Regorafenib-Induced Inhibition of Osteosarcoma Progression In Vitro and In Vivo.

Journal of clinical medicine

Pan PJ,Liu YC,Hsu FT

PA5-29253 was used in Western Blotting to study the efficacy and molecular mechanism of regorafenib on osteoscarcoma in vitro and in vivo.

Mon Jun 24 00:00:00 EDT 2019

Magnolol Induces Apoptosis and Inhibits ERK-modulated Metastatic Potential in Hepatocellular Carcinoma Cells.

In vivo (Athens, Greece)

Kuan LY,Chen WL,Chen JH,Hsu FT,Liu TT,Chen WT,Wang KL,Chen WC,Liu YC,Wang WS

PA5-29253 was used in Western Blotting to evaluate the anti-cancer effect of magnolol in hepatocellular carcinoma cells in vitro.

Mon Jan 28 00:00:00 EST 2019

Bioinformatics

Protein Aliases: Baculoviral IAP repeat-containing protein 4; BIR4; E3 ubiquitin-protein ligase XIAP; HILP; IAP homolog A; IAP-3; IAP-like protein; ILP; Inhibitor of apoptosis protein 3; RING-type E3 ubiquitin transferase XIAP; RP1-315G1.5; X-linked IAP; X-linked inhibitor of apoptosis protein; X-linked inhibitor of apoptosis, E3 ubiquitin protein ligase

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Gene Aliases: 1110015C02Rik; Aipa; API3; BIRC4; hIAP-3; hIAP3; IAP-3; IAP3; ILP-1; ILP1; MIHA; XIAP; XLP2

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UniProt ID: (Human) P98170, (Mouse) Q60989

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Entrez Gene ID: (Human) 331, (Mouse) 11798

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Function(s)

ubiquitin-protein transferase activity protein binding zinc ion binding ligase activity identical protein binding cysteine-type endopeptidase inhibitor activity involved in apoptotic process ubiquitin protein ligase activity protease binding UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity ribosomal S6-glutamic acid ligase activity coenzyme F420-0 gamma-glutamyl ligase activity coenzyme F420-2 alpha-glutamyl ligase activity metal ion binding protein-glycine ligase activity protein-glycine ligase activity, initiating protein-glycine ligase activity, elongating tubulin-glycine ligase activity protein-glutamic acid ligase activity tubulin-glutamic acid ligase activity scaffold protein binding protease inhibitor

Process(es)

apoptotic process cellular response to DNA damage stimulus Wnt signaling pathway protein ubiquitination regulation of BMP signaling pathway positive regulation of protein ubiquitination regulation of cell proliferation negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process regulation of innate immune response regulation of inflammatory response copper ion homeostasis regulation of nucleotide-binding oligomerization domain containing signaling pathway positive regulation of canonical Wnt signaling pathway mitotic spindle assembly positive regulation of protein linear polyubiquitination inhibition of cysteine-type endopeptidase activity involved in apoptotic process negative regulation of neuron apoptotic process neuron apoptotic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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XIAP Polyclonal Antibody

XIAP Antibody (PA5-29253) in ICC/IF

Product Details

PA5-29253

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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