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Invitrogen
alpha Actinin 4 Monoclonal Antibody (7H6)
FIGURE: 1 / 6
alpha Actinin 4 Antibody (MA1-800) in IHC (P)
Immunohistochemistry was performed on cancer biopsies of deparaffinized Human colon carcinoma tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse mono... View More
Product Details
MA1-800
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Mouse
/ IgG
Class
Monoclonal
Type
Antibody
Clone
7H6
Immunogen
Synthetic peptide corresponding to residues A(884) P Y Q G P D A V P G A L D(897) of human alpha Actinin 4.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2221690
Product Specific Information
MA1-800 detects the alpha Actinin 4 in human and mouse cells.
MA1-800 has been successfully used in Western blot, and immunofluorescence procedures. MA1-800 detects alpha Actinin 4 which has a predicted molecular weight of approximately 105 kDa. Under reducing conditions, MA1-800 detects a monomer of alpha Actinin 4 at approximately 52.5 kDa.
The MA1-800 immunogen is a synthetic peptide corresponding to residues A(884) P Y Q G P D A V P G A L D(897) of human alpha Actin 4.
Target Information
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: actinin-Alpha-4; Alpha-actinin-4; F-actin cross-linking protein; focal segmental glomerulosclerosis 1; Non-muscle alpha-actinin 4
Gene Aliases: ACTININ-4; ACTN4; C77391; FSGS; FSGS1
UniProt ID: (Human) O43707, (Mouse) P57780
Entrez Gene ID: (Human) 81, (Mouse) 60595
RNA polymerase II regulatory region sequence-specific DNA binding
nucleoside binding
actin binding
integrin binding
calcium ion binding
protein binding
ligand-dependent nuclear receptor transcription coactivator activity
chromatin DNA binding
nuclear hormone receptor binding
protein homodimerization activity
retinoic acid receptor binding
ion channel binding
poly(A) RNA binding
protein N-terminus binding
actin filament binding
protein complex binding
metal ion binding
transport
protein transport
peroxisome proliferator activated receptor signaling pathway
regulation of apoptotic process
retinoic acid receptor signaling pathway
positive regulation of pinocytosis
actin filament bundle assembly
negative regulation of cellular component movement
positive regulation of cellular component movement
bicellular tight junction assembly
protein localization to bicellular tight junction
regulation of nucleic acid-templated transcription
response to hypoxia
platelet degranulation
vesicle transport along actin filament
positive regulation of cell migration
positive regulation of sodium:proton antiporter activity
negative regulation of substrate adhesion-dependent cell spreading
positive regulation of NIK/NF-kappaB signaling
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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