SiRNA Validation Data

siRNA ID:

110754

Gene ID:

3480

Transcript(s):

NM_000875.4 NM_001291858.1 XM_011521516.2 XM_011521517.2 XM_017022136.1 XM_017022137.1 XM_017022138.1 XM_017022139.1

Validation Results:

19%(+/-13% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00609566_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer® Negative Control #1 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.