120599
NM_001166418.1
NM_001166419.1
NM_001166448.1
NM_018486.2
NR_051952.1
XM_011530986.2
XM_011530987.2
XM_017029640.1
XM_017029641.1
XM_017029642.1
XM_017029644.1
XM_017029646.1
XM_017029647.1
21%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00218503_m1]
HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.