1436
NM_000389.4
NM_001220777.1
NM_001220778.1
NM_001291549.1
NM_078467.2
10%(+/-3% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00355782_m1]
HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.