SiRNA Validation Data

siRNA ID:

s2683

Gene ID:

960

Transcript(s):

NM_000610.3 NM_001001389.1 NM_001001390.1 NM_001001391.1 NM_001001392.1 NM_001202555.1 NM_001202556.1 NM_001202557.1 XM_005253231.2 XM_005253232.2 XM_005253233.2 XM_005253234.2 XM_005253235.2 XM_005253238.2 XM_005253239.2 XM_005253240.2 XM_006718388.1 XM_006718390.3 XM_011520482.1 XM_011520483.1 XM_011520484.1 XM_011520485.1 XM_011520486.1 XM_011520487.2 XM_011520488.1 XM_011520489.2 XM_017018583.1 XM_017018584.1 XM_017018585.1

Validation Results:

5%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00174139_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.