SiRNA Validation Data

siRNA ID:

s22136

Gene ID:

11215

Transcript(s):

NM_016248.3 XM_005266247.1 XM_005266248.3 XM_005266249.3 XM_005266250.3 XM_011534903.2 XM_011534904.1 XM_011534905.1 XM_011534906.2 XM_017020381.1 XM_017020382.1 XM_017020383.1

Validation Results:

14%(+/-3% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00179571_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.