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ZO-3 Antibody (36-4000) in ICC/IF
Immunofluorescent analysis of ZO-3/TJP3 Antibody was done on 90% confluent log phase CACO2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with ZO-3/TJP3 Antibody (Product # 36-4000) at 1µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Goat Anti-Rabbit IgG Secondary Antibody (Product # A-11008) at a dilution of 1:400 for 45 minutes at ro... View More
Product Details
36-4000
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Mouse,
Rat
Published species
Dog,
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide derived from an internal region of the human ZO-3 protein.
Conjugate
Form
Liquid
Concentration
0.25 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2533256
Product Specific Information
36-4000 was used successfully in the western blot analysis of ZO-3 in MDCK cell lysate.
Target Information
ZO-3 is a member of the membrane associated guanylate kinse (MAGUK) family and are considered tight junction-associated proteins. Tight junctions are complexes of proteins that create intercellular boundaries between the plasma membrane domains of epithelial and endothelial cells. ZO-3 is believed to have both structural and signaling roles, and is characteristically defined by three protein-protein interaction modules: the PDZ domain, the SH3 domain and the guanylate kinase (GuK) domain. ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Tight junction protein 3; tight junction protein 3 (zona occludens 3); Tight junction protein ZO-3; TJP-3; Zona occludens protein 3; Zonula occludens protein 3
Gene Aliases: TJP3; ZO-3; ZO3
UniProt ID: (Human) O95049
Entrez Gene ID: (Human) 27134, (Dog) 403852, (Mouse) 27375, (Rat) 314640
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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