Invitrogen

PAX6 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

20 Published Figures

29 References

View all (62) PAX6 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

PAX6 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 31

PAX6 Antibody (42-6600) in ICC/IF

Immunofluorescence analysis of PAX6 was done on 70% confluent log phase U-87MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PAX6 Rabbit Polyclonal Antibody (Product # 42-6600) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at... View More

PAX6 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PAX6 Antibody in Immunohistochemistry (Frozen) (IHC (F))

PAX6 Antibody in Immunohistochemistry (IHC)

PAX6 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

Product Image Gallery

PAX6 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with Pax6 siRNA and decrease in signal intensity was observed in western blot application (Fig a) using PAX6 Polyclonal Antibody (Product # 42-6600). Densitometric analysis of this western blot is shown in histogram (Fig b). {KD}

Product Details

42-6600

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 10 publications 10 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

2-3 µg/mL

View 11 publications 11 publications

Flow Cytometry (Flow)

1:100

View 1 publication 1 publication

Immunoprecipitation (IP)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Human Pax6

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533534

Product Specific Information

42-6600 has been successfully used in flow cytometry and immunofluorescence analysis of Pax6 in human neural stem cells derived from pluripotent stem cells and reacts with human samples.

Target Information

This gene encodes paired box gene 6, one of many human homologs of the Drosophila melanogaster gene prd. In addition to the hallmark feature of this gene family, a conserved paired box domain, the encoded protein also contains a homeo box domain. Both domains are known to bind DNA, and function as regulators of gene transcription. This gene is expressed in the developing nervous system, and in developing eyes. Mutations in this gene are known to cause ocular disorders such as aniridia and Peter's anomaly. Alternatively spliced transcript variants encoding either the same or different isoform have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

De novo genes with an lncRNA origin encode unique human brain developmental functionality.

Nature ecology & evolution

An NA,Zhang J,Mo F,Luan X,Tian L,Shen QS,Li X,Li C,Zhou F,Zhang B,Ji M,Qi J,Zhou WZ,Ding W,Chen JY,Yu J,Zhang L,Shu S,Hu B,Li CY

42-6600 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot to identifiy distinctive U1 elements and RNA splice-related sequences accounting for RNA nuclear export, differentiating mRNAs from lncRNAs, and driving the origin of de novo genes from lncRNA loci.

Wed Feb 01 00:00:00 EST 2023

De novo genes with an lncRNA origin encode unique human brain developmental functionality.

Nature ecology & evolution

An NA,Zhang J,Mo F,Luan X,Tian L,Shen QS,Li X,Li C,Zhou F,Zhang B,Ji M,Qi J,Zhou WZ,Ding W,Chen JY,Yu J,Zhang L,Shu S,Hu B,Li CY

Wed Feb 01 00:00:00 EST 2023

Generation of two iPSC lines from long QT syndrome patients carrying SNTA1 variants.

Stem cell research

Jimenez-Tellez N,Vera CD,Yildirim Z,Vicente Guevara J,Zhang T,Wu JC

42-6600 was used in Immunocytochemistry-immunofluorescence to generate two human-induced pluripotent stem cell (iPSC) lines from two LQT patients carrying a missense mutation in SNTA1 (c.1088A > C).

Wed Feb 01 00:00:00 EST 2023

Generation of two human iPSC lines with Exon 3 mutations in BCL2-Associated Athanogene 3 (BAG3) from dilated cardiomyopathy patients.

Stem cell research

Zushin PH,Zhou Y,Li A,Ashley EA,Wheeler MT,Wu JC

42-6600 was used in Immunohistochemistry to generate two human iPSC lines from individuals with mutations in exon 3 of BAG3 and provide validation of their pluripotency and ability to differentiate toward the three primary germ layers.

Wed Mar 01 00:00:00 EST 2023

Three-dimensional co-culture platform of human induced pluripotent stem cell-derived oligodendrocyte lineage cells and neurons for studying myelination.

STAR protocols

von der Bey M,De Cicco S,Zach S,Hengerer B,Ercan-Herbst E

42-6600 was used in Immunocytochemistry to differentiate hiPSCs into cortical neurons and oligodendrocyte lineage cells on 3D nanofibers.

Fri Mar 17 00:00:00 EDT 2023

Identification of cis-regulatory modules for adeno-associated virus-based cell-type-specific targeting in the retina and brain.

The Journal of biological chemistry

Lin CH,Sun Y,Chan CSY,Wu MR,Gu L,Davis AE,Gu B,Zhang W,Tanasa B,Zhong LR,Emerson MM,Chen L,Ding JB,Wang S

Published figure using PAX6 polyclonal antibody (Product # 42-6600) in Immunohistochemistry (PFA fixed)

Fri Apr 01 00:00:00 EDT 2022

Identification of cis-regulatory modules for adeno-associated virus-based cell-type-specific targeting in the retina and brain.

The Journal of biological chemistry

Lin CH,Sun Y,Chan CSY,Wu MR,Gu L,Davis AE,Gu B,Zhang W,Tanasa B,Zhong LR,Emerson MM,Chen L,Ding JB,Wang S

Published figure using PAX6 polyclonal antibody (Product # 42-6600) in Immunohistochemistry (PFA fixed)

Fri Apr 01 00:00:00 EDT 2022

Nuclear Transporter IPO13 Is Central to Efficient Neuronal Differentiation.

Cells

Gajewska KA,Haynes JM,Jans DA

Published figure using PAX6 polyclonal antibody (Product # 42-6600) in Immunocytochemistry

Sun Jun 12 00:00:00 EDT 2022

Generation of human induced pluripotent stem cell lines carrying heterozygous PLN mutation from dilated cardiomyopathy patients.

Stem cell research

Caudal A,Mondejar-Parreño G,Vera CD,Williams DR,Shenoy SP,Liang D,Wu JC

42-6600 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot to show that these patient-specific iPSC lines serve as valuable in vitro models for DCM pathology caused by PLN mutations.

Mon Aug 01 00:00:00 EDT 2022

Multiplexed genome regulation in vivo with hyper-efficient Cas12a.

Nature cell biology

Guo LY,Bian J,Davis AE,Liu P,Kempton HR,Zhang X,Chemparathy A,Gu B,Lin X,Rane DA,Xu X,Jamiolkowski RM,Hu Y,Wang S,Qi LS

42-6600 was used in In vivo experiments to offer a versatile in vivo tool for a broad range of gene-modulation and gene-therapy applications.

Fri Apr 01 00:00:00 EDT 2022

Establishment and characterization of human pluripotent stem cells-derived brain organoids to model cerebellar diseases.

Scientific reports

Brás J,Henriques D,Moreira R,Santana MM,Silva-Pedrosa R,Adão D,Braz S,Álvaro AR,de Almeida LP,Mendonça LS

42-6600 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot to indicate that the established organoids recapitulate important characteristics of human brain development and exhibit cerebellar features, constituting a resourceful tool for testing therapeutic approaches for cerebellar diseases.

Fri Jul 22 00:00:00 EDT 2022

Generation of two induced pluripotent stem cell lines from dilated cardiomyopathy patients caused by heterozygous mutations in the HCN4 gene.

Stem cell research

Yildirim Z,Kojic A,Yan CD,Wu MA,Vagelos R,Wu JC

42-6600 was used in Immunocytochemistry-immunofluorescence to generate two human induced pluripotent stem cell (iPSC) lines generated from two DCM patients carrying variants in the HCN4 gene (c.2587G > T and c.2846G > A).

Thu Dec 01 00:00:00 EST 2022

The Metabolite Saccharopine Impairs Neuronal Development by Inhibiting the Neurotrophic Function of Glucose-6-Phosphate Isomerase.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Guo Y,Wu J,Wang M,Wang X,Jian Y,Yang C,Guo W

42-6600 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot to demonstrate saccharopine degradation is essential for neuronal development.

Wed Mar 30 00:00:00 EDT 2022

Generation of two induced pluripotent stem cell lines carrying the phospholamban R14del mutation for modeling ARVD/C.

Stem cell research

Vera CD,Manhas A,Shenoy SP,Wheeler MT,Sallam K,Wu JC

42-6600 was used in Immunocytochemistry-immunofluorescence to show two lines carrying this mutation with typical iPSC morphology, pluripotency, karyotype, ability to differentiate into the three germ layers in vitro, and readily availability for studying pathological mechanisms or ARVD/C. Copyright © 2022.

Mon Aug 01 00:00:00 EDT 2022

Characterization of an iPSC line NCHi006-A from a patient with hypoplastic left heart syndrome (HLHS).

Stem cell research

Alonzo M,Contreras J,Ye S,Lin H,Hernandez-Rosario L,McBride KL,Texter K,Garg V,Zhao MT

42-6600 was used in Immunocytochemistry and Flow Cytometry to generante and validate an iPSC line from the peripheral blood mononuclear cells of a male patient with hypoplastic left heart syndrome (HLHS) through Sendai virus-mediated transfection of 4 Yamanaka factors.

Sat Oct 01 00:00:00 EDT 2022

Characterization of an iPSC line NCHi006-A from a patient with hypoplastic left heart syndrome (HLHS).

Stem cell research

Alonzo M,Contreras J,Ye S,Lin H,Hernandez-Rosario L,McBride KL,Texter K,Garg V,Zhao MT

Sat Oct 01 00:00:00 EDT 2022

Compartmentalized microfluidic device for in vitro co-culture of retinal cells.

Biotechnology journal

Jahagirdar D,Yadav S,Gore M,Korpale V,Mathpati CS,Chidambaram S,Majumder A,Jain R,Dandekar P

42-6600 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot to prove the functionality of the retinal coculture, which may be used as an appropriate perfusion-based, preclinical tool for the evaluation of novel retinal drugs and delivery systems.

Thu Sep 01 00:00:00 EDT 2022

Generation of two iPSC lines from hypertrophic cardiomyopathy patients carrying MYBPC3 and PRKAG2 variants.

Stem cell research

Manhas A,Jahng JWS,Vera CD,Shenoy SP,Knowles JW,Wu JC

42-6600 was used in Immunocytochemistry-immunofluorescence to show that both iPSC lines expressed pluripotent markers, had a normal karyotype, and were able to differentiate into three germ layers, making them potentially valuable tools for modeling HCM in vitro and investigating the pathological mechanisms related to these two variants.

Sun May 01 00:00:00 EDT 2022

Aberrant Expression of Circulating MicroRNA Leads to the Dysregulation of Alpha-Synuclein and Other Pathogenic Genes in Parkinson's Disease.

Frontiers in cell and developmental biology

Cai M,Chai S,Xiong T,Wei J,Mao W,Zhu Y,Li X,Wei W,Dai X,Yang B,Liu W,Shu B,Wang M,Lu T,Cai Y,Zheng Z,Mei Z,Zhou Y,Yang J,Zhao J,Shen L,Ho JWK,Chen J,Xiong N

Published figure using PAX6 polyclonal antibody (Product # 42-6600) in Immunocytochemistry

Fri Sep 10 00:00:00 EDT 2021

Maternal Immune Activation Alters Fetal Brain Development and Enhances Proliferation of Neural Precursor Cells in Rats.

Frontiers in immunology

Baines KJ,Hillier DM,Haddad FL,Rajakumar N,Schmid S,Renaud SJ

42-6600 was used in Immunohistochemistry-immunofluorescence to suggest that MIA elicits rapid gene expression changes within the conceptus, including repression of neurodevelopmental pathways, resulting in profound alterations in fetal brain development.

Fri Apr 02 00:00:00 EDT 2021

Maternal Immune Activation Alters Fetal Brain Development and Enhances Proliferation of Neural Precursor Cells in Rats.

Frontiers in immunology

Baines KJ,Hillier DM,Haddad FL,Rajakumar N,Schmid S,Renaud SJ

Fri Apr 02 00:00:00 EDT 2021

Defining multistep cell fate decision pathways during pancreatic development at single-cell resolution.

The EMBO journal

Yu XX,Qiu WL,Yang L,Zhang Y,He MY,Li LC,Xu CR

42-6600 was used in Immunohistochemistry-immunofluorescence to define multistep cell fate decision pathways during pancreatic development at single-cell resolution.

Mon Apr 15 00:00:00 EDT 2019

Computational fluid dynamic analysis of physical forces playing a role in brain organoid cultures in two different multiplex platforms.

BMC developmental biology

Goto-Silva L,Ayad NME,Herzog IL,Silva NP,Lamien B,Orlande HRB,da Costa Souza A,Ribeiro S,Martins M,Domont GB,Junqueira M,Tovar-Moll F,Rehen SK

42-6600 was used in Immunofluorescence in immunohistochemistry on frozen tissues to analyse the physical forces playing a role in brain organoid cultures in two different multiplex platforms.

Thu Mar 07 00:00:00 EST 2019

MicroRNA-365 modulates astrocyte conversion into neuron in adult rat brain after stroke by targeting Pax6.

Glia

Mo JL,Liu Q,Kou ZW,Wu KW,Yang P,Chen XH,Sun FY

Published figure using PAX6 polyclonal antibody (Product # 42-6600) in Immunofluorescence

Sun Jul 01 00:00:00 EDT 2018

MicroRNA-365 modulates astrocyte conversion into neuron in adult rat brain after stroke by targeting Pax6.

Glia

Mo JL,Liu Q,Kou ZW,Wu KW,Yang P,Chen XH,Sun FY

Published figure using PAX6 polyclonal antibody (Product # 42-6600) in Immunofluorescence

Sun Jul 01 00:00:00 EDT 2018

MicroRNA-365 modulates astrocyte conversion into neuron in adult rat brain after stroke by targeting Pax6.

Glia

Mo JL,Liu Q,Kou ZW,Wu KW,Yang P,Chen XH,Sun FY

Published figure using PAX6 polyclonal antibody (Product # 42-6600) in Immunofluorescence

Sun Jul 01 00:00:00 EDT 2018

Comparison of in vitro Neuronal Differentiation Capacity Between Mouse Epiblast Stem Cells Derived From Nuclear Transfer and Naturally Fertilized Embryos.

Frontiers in molecular neuroscience

Li T,Zheng Y,Li Y,Ye D

42-6600 was used in Immunocytochemistry-immunoflourescence to study the molecular and cellular mechanisms causing the inefficient perinatal and postnatal development rates of somatic cell nuclear transfer.

Wed Sep 30 00:00:00 EDT 2020

In Vitro Generation of Vascular Wall-Resident Multipotent Stem Cells of Mesenchymal Nature from Murine Induced Pluripotent Stem Cells.

Stem cell reports

Steens J,Zuk M,Benchellal M,Bornemann L,Teichweyde N,Hess J,Unger K,Görgens A,Klump H,Klein D

Published figure using PAX6 polyclonal antibody (Product # 42-6600) in Immunohistochemistry

Tue Apr 11 00:00:00 EDT 2017

Norrin/Frizzled4 signalling in the preneoplastic niche blocks medulloblastoma initiation.

eLife

Bassett EA,Tokarew N,Allemano EA,Mazerolle C,Morin K,Mears AJ,McNeill B,Ringuette R,Campbell C,Smiley S,Pokrajac NT,Dubuc AM,Ramaswamy V,Northcott PA,Remke M,Monnier PP,Potter D,Paes K,Kirkpatrick LL,Coker KJ,Rice DS,Perez-Iratxeta C,Taylor MD,Wallace VA

Published figure using PAX6 polyclonal antibody (Product # 42-6600) in Immunofluorescence

Tue Nov 08 00:00:00 EST 2016

Development and Dynamic Regulation of Mitochondrial Network in Human Midbrain Dopaminergic Neurons Differentiated from iPSCs.

Stem cell reports

Fang D,Qing Y,Yan S,Chen D,Yan SS

Published figure using PAX6 polyclonal antibody (Product # 42-6600) in Western Blot

Tue Oct 11 00:00:00 EDT 2016

Mfn2 is Required for Mitochondrial Development and Synapse Formation in Human Induced Pluripotent Stem Cells/hiPSC Derived Cortical Neurons.

Scientific reports

Fang D,Yan S,Yu Q,Chen D,Yan SS

42-6600 was used in immunocytochemistry and western blot study mitochondrial development, neurogenesis, and synapse formation in human induced pluripotent stem cell-derived cortical neurons

Thu Aug 18 00:00:00 EDT 2016

De-SUMOylation of CCCTC binding factor (CTCF) in hypoxic stress-induced human corneal epithelial cells.

The Journal of biological chemistry

Wang J,Wang Y,Lu L

42-6600 was used in western blot to report that regulation of CTCF by de-SUMOylation is dependent upon hypoxic and oxidative stresses.

Fri Apr 06 00:00:00 EDT 2012

De-SUMOylation of CCCTC binding factor (CTCF) in hypoxic stress-induced human corneal epithelial cells.

The Journal of biological chemistry

Wang J,Wang Y,Lu L

42-6600 was used in western blot to report that regulation of CTCF by de-SUMOylation is dependent upon hypoxic and oxidative stresses.

Fri Apr 06 00:00:00 EDT 2012

De-SUMOylation of CCCTC binding factor (CTCF) in hypoxic stress-induced human corneal epithelial cells.

The Journal of biological chemistry

Wang J,Wang Y,Lu L

42-6600 was used in western blot to report that regulation of CTCF by de-SUMOylation is dependent upon hypoxic and oxidative stresses.

Fri Apr 06 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: Aniridia; Aniridia type II protein; Dickie's small eye; Keratitis; MGC17209; Oculorhombin; paired box gene 6; Paired Box Gene-6; paired box homeotic gene 6; paired box homeotic gene-6; Paired box protein Pax-6; small eye

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Gene Aliases: 1500038E17Rik; AEY11; AN; AN2; D11S812E; Dey; FVH1; Gsfaey11; MGDA; Pax-6; PAX6; Sey; WAGR

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UniProt ID: (Human) P26367, (Rat) P63016, (Mouse) P63015

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Entrez Gene ID: (Human) 5080, (Rat) 25509, (Mouse) 18508

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding ubiquitin-protein transferase activity protein binding transcription factor binding protein kinase binding ubiquitin protein ligase binding histone acetyltransferase binding co-SMAD binding R-SMAD binding HMG box domain binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding AT DNA binding double-stranded DNA binding sequence-specific DNA binding transcription regulatory region DNA binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PAX6 Polyclonal Antibody

PAX6 Antibody (42-6600) in ICC/IF

Product Details

42-6600

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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