Invitrogen

Cdc42 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

4 Published Figures

9 References

View all (16) CDC42 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Cdc42 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 13

Cdc42 Antibody (PA1-092) in ICC/IF

Immunofluorescent analysis of cdc42 (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 5% normal goat serum (Product # 31873) for 15 minutes at room temperature. Cells were probed with a cdc42 polyclonal antibody (Product # PA1-092) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:400 for 30 minutes at room te... View More

Cdc42 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Cdc42 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

Product Image Gallery

Cdc42 Antibody — Fig 2 Knockdown of selected genes required for ANDV infection. Western blots of indicated proteins after knockdown with gene-specific siRNA against (A) dynamin 2 (DNM2); (B) clathrin heavy chain (CLTC); (C) AP2M1; (D) caveolin 1 (CAV1); (E) CDC42; (F) ARF6; (G) NSF; (H) RAB5C; or (I) TSG101; or non-targeting siRNA transfection control (NT). siRNAs were transfected into HMVEC-L at the concentration of 100 nM for 48 h (see Table 1 for more information regarding these genes). The cells were then infected with ANDV (MOI = 0.5) for 24 h or 48 h. Western blots were performed post infection to ensure knockdown of the specific protein expression. Molecular weight of each specific protein is indicated on the right side of each panel. The blots were also probed with beta-actin specific antibody as the gel-loading control. (A) and (B) show only the results collected at 48 h. {KD}

Product Details

PA1-092

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20

Immunocytochemistry (ICC/IF)

1:100

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

C-Terminal truncated human recombinant protein

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA, 30% glycerol

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2539858

Target Information

Rac1 is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for the Rac1 gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A nuclear function for an oncogenic microRNA as a modulator of snRNA and splicing.

Molecular cancer

El Fatimy R,Zhang Y,Deforzh E,Ramadas M,Saravanan H,Wei Z,Rabinovsky R,Teplyuk NM,Uhlmann EJ,Krichevsky AM

Published figure using Cdc42 polyclonal antibody (Product # PA1-092) in Western Blot

Sat Jan 15 00:00:00 EST 2022

A nuclear function for an oncogenic microRNA as a modulator of snRNA and splicing.

Molecular cancer

El Fatimy R,Zhang Y,Deforzh E,Ramadas M,Saravanan H,Wei Z,Rabinovsky R,Teplyuk NM,Uhlmann EJ,Krichevsky AM

Published figure using Cdc42 polyclonal antibody (Product # PA1-092) in Western Blot

Sat Jan 15 00:00:00 EST 2022

Cdc42 negatively regulates endocytosis during apical membrane maintenance in live animals.

Molecular biology of the cell

Shitara A,Malec L,Ebrahim S,Chen D,Bleck C,Hoffman MP,Weigert R

Fri Feb 01 00:00:00 EST 2019

Phenotypic characterisation of breast cancer: the role of CDC42.

Breast cancer research and treatment

Chrysanthou E,Gorringe KL,Joseph C,Craze M,Nolan CC,Diez-Rodriguez M,Green AR,Rakha EA,Ellis IO,Mukherjee A

PA1092 was used in immunohistochemistry - paraffin section and western blot to study the impact of cell division control 42 homolog on clinicopathological significance Luminal A subtype breast cancer

Sat Jul 01 00:00:00 EDT 2017

Phenotypic characterisation of breast cancer: the role of CDC42.

Breast cancer research and treatment

Chrysanthou E,Gorringe KL,Joseph C,Craze M,Nolan CC,Diez-Rodriguez M,Green AR,Rakha EA,Ellis IO,Mukherjee A

PA1092 was used in immunohistochemistry - paraffin section and western blot to study the impact of cell division control 42 homolog on clinicopathological significance Luminal A subtype breast cancer

Sat Jul 01 00:00:00 EDT 2017

MicroRNA-27a Inhibits Cell Migration and Invasion of Fibroblast-Like Synoviocytes by Targeting Follistatin-Like Protein 1 in Rheumatoid Arthritis.

Molecules and cells

Shi DL,Shi GR,Xie J,Du XZ,Yang H

PA1-092X was used in western blot to investigate the effects of miR-27a on cell migration and invasion in fibroblast-like synoviocytes from rheumatoid arthritis patients

Wed Aug 31 00:00:00 EDT 2016

The localisation of the apical Par/Cdc42 polarity module is specifically affected in microvillus inclusion disease.

Biology of the cell

Michaux G,Massey-Harroche D,Nicolle O,Rabant M,Brousse N,Goulet O,Le Bivic A,Ruemmele FM

PA1-092 was used in immunohistochemistry - paraffin section to determine the apical polarity determinants in five patients with microvillus inclusion disease

Fri Jan 01 00:00:00 EST 2016

The NarE protein of Neisseria gonorrhoeae catalyzes ADP-ribosylation of several ADP-ribose acceptors despite an N-terminal deletion.

FEMS microbiology letters

Rodas PI,Álamos-Musre AS,Álvarez FP,Escobar A,Tapia CV,Osorio E,Otero C,Calderón IL,Fuentes JA,Gil F,Paredes-Sabja D,Christodoulides M

PA1-092X was used in western blot to determine the enzymatic activity of NarE of Neisseria gonorrhoeae

Thu Sep 01 00:00:00 EDT 2016

Endocytic Pathways Used by Andes Virus to Enter Primary Human Lung Endothelial Cells.

PloS one

Chiang CF,Flint M,Lin JS,Spiropoulou CF

Published figure using Cdc42 polyclonal antibody (Product # PA1-092) in Western Blot

Tue Jun 13 00:00:00 EDT 2017

14-3-3ε and ζ regulate neurogenesis and differentiation of neuronal progenitor cells in the developing brain.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Toyo-oka K,Wachi T,Hunt RF,Baraban SC,Taya S,Ramshaw H,Kaibuchi K,Schwarz QP,Lopez AF,Wynshaw-Boris A

PA1-092X was used in immunocytochemistry and immunohistochemistry to examine the role of 14-3-3 proteins during brain development

Wed Sep 03 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: Cell division control protein 42 homolog; cell division cycle 42 (GTP binding protein); cell division cycle 42 homolog; dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD)); dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD)); G25K GTP-binding protein; growth-regulating protein; GTP binding protein, 25kDa; RP1-224A6.5; small GTP binding protein CDC42

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Gene Aliases: AI747189; AU018915; CDC42; CDC42Hs; G25K; TKS

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UniProt ID: (Human) P60953, (Rat) Q8CFN2, (Mouse) P60766

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Entrez Gene ID: (Human) 998, (Rat) 64465, (Mouse) 12540

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Function(s)

GTPase activity protein binding GTP binding protein kinase binding GTP-dependent protein binding mitogen-activated protein kinase kinase kinase binding thioesterase binding apolipoprotein A-I receptor binding identical protein binding Rho GDP-dissociation inhibitor binding ubiquitin protein ligase activity nucleotide binding small GTPase

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Cdc42 Polyclonal Antibody

Cdc42 Antibody (PA1-092) in ICC/IF

Product Details

PA1-092

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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