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Invitrogen
H2BK12ac Polyclonal Antibody
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 14
H2BK12ac Antibody (PA5-40100) in ChIP-seq
ChIP was performed on sheared chromatin from 1.5 million HeLaS3 cells using 0.5 µg of Acetyl-Histone H2B (Lys12) polyclonal antibody (Product # PA5-40100). The IP'd DNA was subsequently analyzed on a HiSeq. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1 Mb region of the X-chromosome (fig 2A and B) and in genomic regions of chromosome 7, surrounding the ACTB gene, and of chromosome 12, surrounding the GAPDH gene (fig 2C and D). The position of the am... View More
Product Details
PA5-40100
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH-conjugated synthetic peptide corresponding to human the region of histone H2B containing the acetylated lysine 12 (H2BK12ac).
Conjugate
Form
Liquid
Concentration
0.82 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.05% sodium azide, 0.05% ProClin 300
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2608354
Target Information
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The nucleosomes wrap further and compact DNA into chromotin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones play a central role in transciption regulation, DNA repair, DNA replication, and chromosomal stability. H2B has a broad antibacterial activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Acetyl-Histone H2; Acetyl-Histone H2B K12; Acetyl-Histone H2B Lys12; H2B GL105; H2B histone family member W testis-specific; H2B histone family, member A; H2B histone family, member B; H2B histone family, member C; H2B histone family, member D; H2B histone family, member E; H2B histone family, member F; H2B histone family, member J; H2B histone family, member K; H2B histone family, member L; H2B histone family, member M; H2B histone family, member N; H2B histone family, member Q; H2B histone family, member R; H2B histone family, member T; H2B histone family, member U, (testis-specific); H2B histone family, member W, testis-specific; H2B K; H2B type 12; H2B-clustered histone 12 like; H2B-clustered histone 17; H2B-clustered histone 18; H2B-clustered histone 21; H2B-clustered histone 26; H2B-clustered histone 3; H2B-clustered histone 5; H2B-clustered histone 9; H2B.S histone 1; H2B.U histone 1; H2B.W histone 1; H2B/a; H2B/b; H2B/c; H2B/d; H2B/e; H2B/f; H2B/g; H2B/h; H2B/I; H2B/j; H2B/k; H2B/l; H2B/n; H2B/q; H2B/r; H2B/s; H2BK12ac; H2BK15ac; H2BK5ac; H2BLys12ac; H2BS14; H2BS14p; HIRA-interacting protein 1; HIRA-interacting protein 2; HIST1H3I; histone 1, H2ba; histone 1, H2bb; histone 1, H2bc; histone 1, H2bd; histone 1, H2bg; histone 1, H2bh; histone 1, H2bi; histone 1, H2bj; histone 1, H2bk; histone 1, H2bl; histone 1, H2bm; histone 1, H2bn; histone 1, H2bo; histone 2, H2be; Histone 2B; histone 3, H2bb; histone family member; Histone H2B type 1-A; Histone H2B type 1-B; Histone H2B type 1-C/E/F/G/I; Histone H2B type 1-D; Histone H2B type 1-H; Histone H2B type 1-J; Histone H2B type 1-K; Histone H2B type 1-L; Histone H2B type 1-M; Histone H2B type 1-N; Histone H2B type 1-O; Histone H2B type 2-E; Histone H2B type 2-F; Histone H2B type 3-B; Histone H2B type F-M; Histone H2B type F-S; histone H2B type F-S-like; Histone H2B type W-T; Histone H2B, testis; Histone H2B-GL105; Histone H2B.1; Histone H2B.1 A; Histone H2B.1 B; Histone H2B.2; Histone H2B.a; Histone H2B.b; Histone H2B.c; Histone H2B.d; Histone H2B.e; Histone H2B.f; Histone H2B.g; Histone H2B.h; Histone H2B.I; Histone H2B.j; Histone H2B.k; Histone H2B.l; Histone H2B.n; Histone H2B.q; Histone H2B.r; Histone H2B.s; MGC125414; MGC125415; MGC125416; MGC9388; RP1-193B12.10; Testis-specific histone H2B; TSH2B.1
Gene Aliases: bA317E16.3; dJ160A22.3; dJ193B12.2; dJ221C16.3; dJ221C16.6; dJ221C16.8; dJ97D16.4; GL105; H2B; H2B.1; H2B.1A; H2B.1B; H2B.2; H2B/a; H2B/b; H2B/c; H2B/d; H2B/e; H2B/f; H2B/j; H2B/k; H2B/l; H2B/n; H2B/r; H2B/S; H2Bb; H2BC1; H2BC10; H2BC11; H2BC12; H2BC12L; H2BC13; H2BC14; H2BC15; H2BC17; H2BC18; H2BC21; H2BC26; H2BC3; H2BC4; H2BC5; H2BC6; H2BC7; H2BC8; H2BC9; H2BFA; H2BFAiii; H2BFB; H2BFC; H2BFD; H2BFE; H2BFF; H2BFG; H2BFH; H2BFJ; H2BFK; H2BFL; H2BFM; H2BFN; H2BFQ; H2BFR; H2BFS; H2BFT; H2BFU; H2BFWT; H2BGL105; H2BJ; H2BK; H2BQ; H2BS1; H2BU1; H2BW1; H2BW2; HIRIP1; HIRIP2; HIST1H2BA; HIST1H2BB; HIST1H2BC; HIST1H2BD; HIST1H2BE; HIST1H2BF; HIST1H2BG; HIST1H2BH; HIST1H2BI; HIST1H2BJ; HIST1H2BK; HIST1H2BL; HIST1H2BM; HIST1H2BN; HIST1H2BO; HIST2H2BE; HIST2H2BF; HIST3H2BB; STBP; TH2B; TH2B-175; TSH2B; TSH2B.1
nucleosome assembly
nucleosome disassembly
inflammatory response
plasminogen activation
spermatogenesis, exchange of chromosomal proteins
positive regulation of binding
mononuclear cell migration
innate immune response in mucosa
antibacterial humoral response
defense response to Gram-positive bacterium
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