Invitrogen

Collagen I Polyclonal Antibody

Collagen I Antibody in Immunocytochemistry (ICC/IF)

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Collagen I Antibody (PA5-95137) in ICC/IF

Immunofluorescence analysis of Collagen I in NIH3T3 cell. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with Collagen I polyclonal antibody (Product # PA5-95137) at a 1 µg/mL dilution, followed by biotinylated goat anti-rabbit IgG (30 min, 37°C) using a dilution and developed with Strepavidin-Biotin-Complex/DAB.

Collagen I Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Collagen I Antibody in Western Blot (WB)

Product Image Gallery

Product Details

PA5-95137

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.5 µg/mL

View 7 publications 7 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1-2 µg/mL

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

5 µg/mL

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of mouse Collagen I (1192-1207aa QPPQEKSQDGGRYYRA).

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 4mg trehalose

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2806942

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Target Information

Collagen I is a Type I collagen with a triple helix structure comprised of two alpha-1 chains and one alpha-2 chain. Collagen I is a member of group I collagen (fibril-forming collagen) found in most connective tissues, and is abundant in bone, cornea, dermis and tendon. Mutations in the COL1A2 gene encoding the alpha-2 chain are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in the COL1A2 gene which codes for the alpha-2 chain, however, tend to be less severe than mutations in the COL1A1 gene, reflecting the different role of alpha-2 chains in matrix integrity. Reciprocal translocations between chromosomes 17 and 22, where Collagen Type 1 genes and the gene for platelet derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Non-muscle myosins control radial glial basal endfeet to mediate interneuron organization.

PLoS biology

D'Arcy BR,Lennox AL,Manso Musso C,Bracher A,Escobar-Tomlienovich C,Perez-Sanchez S,Silver DL

PA5-95137 was used in Immunohistochemistry to demonstrate the utility of in vivo proximity labeling for dissecting local control of complex systems and reveals new mechanisms for dictating RGC integrity and cortical architecture.

Wed Feb 01 00:00:00 EST 2023

Fibroblast Activation Protein Activates Macrophages and Promotes Parenchymal Liver Inflammation and Fibrosis.

Cellular and molecular gastroenterology and hepatology

Yang AT,Kim YO,Yan XZ,Abe H,Aslam M,Park KS,Zhao XY,Jia JD,Klein T,You H,Schuppan D

PA5-95137 was used in Western Blotting and Immunohistochemistry to show that pharmacological fibroblast activation protein (FAP) inhibition attenuates inflammation-predominant liver fibrosis. FAP is expressed on subsets of activated fibroblasts/hepatic stellate cells (HSC) and promotes both macrophage and HSC profibrogenic activity in liver fibrosis.

Tue Mar 21 00:00:00 EDT 2023

Fibroblast Activation Protein Activates Macrophages and Promotes Parenchymal Liver Inflammation and Fibrosis.

Cellular and molecular gastroenterology and hepatology

Yang AT,Kim YO,Yan XZ,Abe H,Aslam M,Park KS,Zhao XY,Jia JD,Klein T,You H,Schuppan D

Tue Mar 21 00:00:00 EDT 2023

FBXL6 is dysregulated in keloids and promotes keloid fibroblast growth by inducing c-Myc expression.

International wound journal

Feng G,Sun H,Piao M

PA5-95137 was used in Western Blotting to investigate the role of FBXL6 in keloid fibroblasts, particularly its impact on cell proliferation and collagen deposition, and to explore its potential as a therapeutic target for keloid treatment.

Sun Jan 01 00:00:00 EST 2023

Muscle stem cells and fibro-adipogenic progenitors in female pelvic floor muscle regeneration following birth injury.

NPJ Regenerative medicine

Sesillo FB,Rajesh V,Wong M,Duran P,Rudell JB,Rundio CP,Baynes BB,Laurent LC,Sacco A,Christman KL,Alperin M

PA5-95137 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot to demonstrate that the loss of function and decreased expansion of MuSCs and FAPs after birth injury lead to impaired PFM recovery.

Fri Dec 16 00:00:00 EST 2022

Paclitaxel Induces Epidermal Molecular Changes and Produces Subclinical Alterations in the Skin of Gynecological Cancer Patients.

Cancers

Montero P,Pérez-Leal M,Pérez-Fidalgo JA,Sanz C,Estornut C,Roger I,Milara J,Cervantes A,Cortijo J

PA5-95137 was used in Western Blot to suggest that paclitaxel modifies gene and protein expression of skin-related molecular markers, and impairs different physical, physiological, and biomechanical properties of the skin of cancer patients at a subclinical level.

Wed Feb 23 00:00:00 EST 2022

Development of skeletal muscle atrophy and intermuscular adipose tissue in patients with early breast cancer treated with chemotherapy.

American journal of physiology. Cell physiology

Mallard J,Hucteau E,Bender L,Charlot A,Debrut L,Pflumio C,Trensz P,Schott R,Favret F,Pivot X,Hureau TJ,Pagano AF

PA5-95137 was used in Western Blotting to demonstrate both IMAT development and muscle atrophy in patients with breast cancer who were treated with chemotherapy.

Sat Oct 01 00:00:00 EDT 2022

Additively manufactured BaTiO₃ composite scaffolds: A novel strategy for load bearing bone tissue engineering applications.

Materials science & engineering. C, Materials for biological applications

Mancuso E,Shah L,Jindal S,Serenelli C,Tsikriteas ZM,Khanbareh H,Tirella A

PA5-95137 was used in Immunocytochemistry to conclude that the single-step multi-material additive manufacturing technology used for the fabrication of electroactive PCL/BaTiO3 composite scaffolds holds great promise for sustainability (reduced material waste and manufacturing costs) and it importantly suggests PCL/BaTiO3 scaffolds as promising candidates for load bearing bone tissue engineering applications to solve unmet clinical needs.

Thu Jul 01 00:00:00 EDT 2021

mRNA and long non-coding RNA expression profiling of human periodontal ligament cells under tension loading.

European journal of orthodontics

Lin Y,Cheng T,Zhu S,Gu M,Jin L,Yang Y

Published figure using Collagen I polyclonal antibody (Product # PA5-95137) in Western Blot

Wed Dec 01 00:00:00 EST 2021

LTBP4 affects renal fibrosis by influencing angiogenesis and altering mitochondrial structure.

Cell death & disease

Su CT,Jao TM,Urban Z,Huang YJ,See DHW,Tsai YC,Lin WC,Huang JW

Published figure using Collagen I polyclonal antibody (Product # PA5-95137) in Western Blot

Wed Oct 13 00:00:00 EDT 2021

Leflunomide Induces Dose-Dependent Lung Injury in Mice via Stimulating Vimentin and NLRP3 Inflammasome Production.

Frontiers in pharmacology

El-Sherbiny M,Atef H,Eladl MA,Mohamed AS,El-Shafey M,Ali HS,Zaitone SA,Alomar SY,Alqahtani SAM,Aloyouni SY,Attia MA

PA5-95137 was used in Western Blot to examine the dose-dependent lung injury produced by leflunomide in healthy mice.

Tue May 18 00:00:00 EDT 2021

Bioinformatics

Protein Aliases: alpha 2 type I procollagen; alpha 2(I) procollagen; alpha 2(I)-collagen; alpha-1 type 1 collagen; Alpha-1 type I collagen; Alpha-2 type I collagen; alpha1(I) procollagen; COL1; collagen alpha 1 chain type I; Collagen alpha-1(I) chain; collagen alpha-1(I) chain preproprotein; Collagen alpha-2(I) chain; collagen COL1A2; collagen I, alpha-2 polypeptide; collagen of skin, tendon and bone, alpha-1 chain; collagen of skin, tendon and bone, alpha-2 chain; collagen, type 1, alpha 1; collagen, type I, alpha 1; collagen, type I, alpha 2; osteogenesis imperfecta; pro-alpha-1 collagen type 1; procollagen type I, alpha 1; procollagen, type 1, alpha 1; procollagen, type I, alpha 1; procollagen, type I, alpha 2; Type I Collagen; type I proalpha 1; type I procollagen; type I procollagen alpha 1 chain; type I-alpha 1 collagen

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Gene Aliases: AA960264; AI325291; Col1a-1; Col1a-2; COL1A1; COL1A2; Cola-1; Cola-2; Cola1; Cola2; COLIA1; EDSC; Mov-13; Mov13; OI1; OI2; OI3; OI4; oim

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UniProt ID: (Human) P02452, (Human) P08123, (Rat) P02454, (Mouse) P11087, (Mouse) Q01149, (Rat) P02466

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Entrez Gene ID: (Human) 1277, (Human) 1278, (Rat) 29393, (Mouse) 12842, (Mouse) 12843, (Rat) 84352

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Function(s)

extracellular matrix structural constituent protein binding identical protein binding metal ion binding platelet-derived growth factor binding protein binding, bridging SMAD binding molecular_function extracellular matrix structural protein

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Collagen I Polyclonal Antibody

Collagen I Antibody (PA5-95137) in ICC/IF

Product Details

PA5-95137

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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