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Bovine IFN-gamma Protein (RBOIFNGI) in ELISA
Cytoxicity Bioassay analysis of Bovine IFN-gamma was performed in a viral challenge assay using VS virus on MDBK cells. Various concentrations of Bovine IFN-gamma, ranging from 1.22-2500 ng/mL (lyophilized, blue) or 0.12-250 ng/mL (solution, red), were added to the cultures. Following incubation, plates were first stained with crystal violet for visual CPE determination, and then the dye was solubilized for absorbance readings. Percentage cytoprotection was determined and compared to cytoprotection achieved with IFN-alpha standard.
Product Details
RBOIFNGI
Applications
Tested Dilution
Publications
Product Specifications
Species
Bovine
Expression System
P. pastoris
Amino acid sequence
Bovine IFNγ, amino acids 24 - 166 (Accession # AAB64477).
Molecular weight
16.9 kDa
Class
Recombinant
Type
Protein
Purity
>95% by SDS-PAGE analysis
Activity
The biological activity of recombinant bovine IFN-gamma was determined in a viral challenge assay using VSV on MDBK cells. Plates were stained with crystal violet, a visual CPE determination was performed and the dye was then solubilized and absorbance was read. Treatment of MDBK cells with 6 ng/mL of bovine IFN-gamma causes 25% Cytoprotection in the assay.
Conjugate
Form
Lyophilized
Purification
purified
Storage buffer
PBS
Contains
no preservative
Storage conditions
4° C
Shipping conditions
Wet ice
Product Specific Information
Mature bovine IFNγ was expressed in Pichia pastoris, X-33. GenBank® Accession # AAB64477, amino acids 24 - 166. For protein verification, the expressed protein was N-terminal sequenced and begins with the following 10 amino acids: QGQFFREIEN. >95% by SDS-PAGE analysis.
Activity: The biological activity of recombinant bovine IFNγ was determined in a viral challenge assay using VS virus on MDBK cells. Plates were stained with crystal violet, a visual CPE determination was performed and the dye was then solubilized and absorbance was read.
Uses: For in vitro applications. A dose-response assay should be performed to determine the optimal concentration for specific experiments.
Reconstitution: Reconstitute lyophilized material with 0.5 mL of distilled or deionized water. Dilute in a buffer containing carrier protein, aliquot, and store at = -70°C. Avoid multiple freeze-thaw cycles. PBS with 1% BSA or a neutral media containing = 10% FCS are suitable buffers.
Target Information
IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BoIFNG; H-IFN-g; IFN γ; IFN-gamma; IFN-y; IFNγ; Interferon gamma; interferon gamma type 2; Interferon y; Interferon γ; interferon-gamma precursor; Interferonγ; M-IFN-g; R-IFN-g
Gene Aliases: IFNG
UniProt ID: (Bovine) P07353
Entrez Gene ID: (Bovine) 281237
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