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Product Details
RBOTNFAI
Applications
Tested Dilution
Publications
Product Specifications
Species
Bovine
Published species
Not Applicable
Expression System
P. pastoris
Amino acid sequence
Bovine TNF-alpha, amino acids 77-233 (Accession # Q06599)
Molecular weight
17.4 kDa
Class
Recombinant
Type
Protein
Activity
The biological activity of recombinant bovine TNF alpha was measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells. The ED50 for this effect is typically 5-8 ng/mL.
Conjugate
Form
Lyophilized
Purification
purified
Storage buffer
PBS
Contains
no preservative
Storage conditions
4° C
Shipping conditions
Wet ice
Product Specific Information
For dilution, use a buffer containing carrier protein, e.g. PBS with 1% BSA or a neutral media containing 10% FCS and freeze in aliquots at -70°C.
Activity: The biological activity of recombinant bovine TNF alpha was measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells. The ED50 for this effect is typically 5-8 ng/mL.
Expression: The mature, soluble form of bovine TNFa was expressed in Pichia pastoris, KM71H and therefore does not have endotoxin.
Reconstitute with 0.5 mL of distilled water.
Target Information
TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Cachectin; DADB-70P7.1; TNF alpha; TNF superfamily; TNF α; TNF-a; TNF-alpha; TNFα; Tumor necrosis factor; tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2
Gene Aliases: TNF; TNF-a; TNF-alpha; TNFA; TNFSF2
UniProt ID: (Bovine) A4IFT5
Entrez Gene ID: (Bovine) 280943
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