Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Product Details
14-8943-80
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
Human BAFF, amino acids Ala134-Leu285 (Accession # Q9Y275)
Molecular weight
18.5 kDa
Class
Recombinant
Type
Protein
Purity
>90% by reducing SDS-PAGE
Endotoxin concentration
<0.40 ng/µg
Activity
ED50 = 1.6 µg/mL; determined by a mouse splenocyte survival assay.
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Purification
purified
Storage buffer
PBS
Contains
no preservative
Storage conditions
-80°C
Shipping conditions
Dry ice
Product Specific Information
Description: Recombinant human BAFF (B-cell activating factor), also referred to as BlyS, is a type II transmembrane protein of the TNF superfamily. BAFF is found on T cells, macrophages and dendritic cells. BAFF contains a cytoplasmic domain, transmembrane domain and extracellular domain which can be cleaved between amino acid 133 and 134 to produce a soluble form detectable in serum. Receptors for BAFF include TACI, BAMBI and BAFFR. BAFF/BLyS is a B lymphocyte stimulatory molecule; it induces B cell proliferation and immunoglobulin secretion. In addition BAFF can provide a co-stimulation signal to T lymphocytes. Elevated levels of BAFF has been implicated in the pathogenesis of some autoimmune diseases (particularly) B cell diseases such as systemic lupus erythaematosus. Human and mouse BAFF share 86% amino acid sequence identity.
Applications Reported: Recombinant human BAFF is biologically active.
Applications Tested: The ED50, as determined by a mouse splenocytes survival assay, is 1.6 µg/mL.
Source: E. coli expressed amino acids Ala134-Leu285 accession # Q9Y275.
Bioactivity: The ED50, as determined by a mouse splenocyte survival assay, is 1.6 µg/mL.
Endotoxin: Less than 0.40 ng/ug as determined by the LAL assay. Purity: >90% as determined by reducing SDS-PAGE.
Molecular Weight: 18.5 kDa.
Storage and handling: For best recovery, quick-spin vial prior to opening. Use in a sterile environment.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
BAFF is a membrane bound cytokine expressed by B cell lineage cells. The soluble form of the cytokine binds to the receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. BAFF is a potent B cell activator, and important for the proliferation and differentiation of B cells. BAFF is also expressed by T lymphocytes and has been shown to promote T cell activation and survival. Isoforms representing alternatively spliced, and glycosylated variants have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: ApoL related ligand TALL-1; B lymphocyte stimulator; B-cell-activating factor; B-lymphocyte stimulator; BAFF; BLyS; CD257; delta BAFF; Delta4 BAFF; Dendritic cell-derived TNF-like molecule; TALL-1; TNF and ApoL-related leukocyte expressed ligand 1; TNF homolog that activates apoptosis; TNF- and APOL-related leukocyte expressed ligand 1; tumor necrosis factor (ligand) superfamily member 13b; tumor necrosis factor (ligand) superfamily, member 13b; tumor necrosis factor (ligand) superfamily, member 20; tumor necrosis factor ligand 7A; Tumor necrosis factor ligand superfamily member 13B; tumor necrosis factor-like protein ZTNF4
Gene Aliases: BAFF; BLYS; CD257; DTL; TALL-1; TALL1; THANK; TNFSF13B; TNFSF20; TNLG7A; UNQ401/PRO738; ZTNF4
UniProt ID: (Human) Q9Y275
Entrez Gene ID: (Human) 10673
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support