Gibco

Human CNTF, Animal-Free Recombinant Protein, PeproTech®

Product Details

AF-450-13-20UG

Applications

Tested Dilution

Publications

Functional Assay (Functional)

Assay-dependent

In vitro Assay (IV)

View 4 publications 4 publications

Product Specifications

Species

Human

Published species

Human, Mouse, Non-human primate

Expression System

E. coli

Amino acid sequence

AFTEHSPLTP HRRDLCSRSI WLARKIRSDL TALTQSYVKH QGLNKNINLD SADGMPVAST DQWSQLTQAQ RLQQNLQAYR TFHVLLARLL QDQQVHFTPT QGDFHQAIHT LLLQVAAFAY QIQQLMILLQ YKIPRNQADG MPINVGDGGL FQKKLWGLKV LQQLSQWTVR SIHDLRFISS HQTGIPARGS HYIANNKKM

Molecular weight

22.8 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<0.1 EU/µg

Activity

Determined by its ability to stimulate proliferation of human TF-1 cells using an ED50 concentration range of 50-150 ng/ml.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Human CNTF is synthesized as a 199 amino acid polypeptide (22.8 kDa) lacking a hydrophobic N-terminal signal for secretion.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

The protein encoded by this gene is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. A read-through transcript variant composed of ZFP91 and CNTF sequence has been identified, but it is thought to be non-coding. Read-through transcription of ZFP91 and CNTF has also been observed in mouse.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Establishment of autaptic culture with human-induced pluripotent stem cell-derived astrocytes.

iScience

Uchino K,Tanaka Y,Kawaguchi S,Kubota K,Watanabe T,Katsurabayashi S,Hirose S,Iwasaki K

AF-450-13 was used in Cell Culture to find a significantly higher Na+ current amplitude, membrane capacitance, and number of synapses, as well as longer dendrites, in HiAACs compared with neuron monocultures and show that the system can facilitate the study of CNS diseases and advance the development of drugs targeting glial cells.

Fri Aug 19 00:00:00 EDT 2022

Circ-Hdgfrp3 shuttles along neurites and is trapped in aggregates formed by ALS-associated mutant FUS.

iScience

D'Ambra E,Santini T,Vitiello E,D'Uva S,Silenzi V,Morlando M,Bozzoni I

AF-450-13 was used in In vitro experiments to investigate Circ-Hdgfrp3 and its association with motor neurons carrying ALS-associated FUS mutations.

Fri Dec 17 00:00:00 EST 2021

CRISPR-mediated gene correction links the ATP7A M1311V mutations with amyotrophic lateral sclerosis pathogenesis in one individual.

Communications biology

Yun Y,Hong SA,Kim KK,Baek D,Lee D,Londhe AM,Lee M,Yu J,McEachin ZT,Bassell GJ,Bowser R,Hales CM,Cho SR,Kim J,Pae AN,Cheong E,Kim S,Boulis NM,Bae S,Ha Y

Mon Jan 20 00:00:00 EST 2020

Molecular Classification and Comparative Taxonomics of Foveal and Peripheral Cells in Primate Retina.

Cell

Peng YR,Shekhar K,Yan W,Herrmann D,Sappington A,Bryman GS,van Zyl T,Do MTH,Regev A,Sanes JR