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Product Details
RP-110047
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
EEFGQLYGYRRAAGGVPSMGREIRSEEPEESVPFSCDWRKVAGAISPIKDQKNCNCCWAMAAAGNIETLWRISFWD
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
PBS/1M urea, pH 7.4
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (53%), Rat (53%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-144943. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
Cathepsin W (lymphopain) and cathepsin F comprise a novel subgroup of cathepsin proteases, and are phylogenetically distinct from other human cathepsins. The cathepsin W gene maps to chromosome 11q13.1 and contains ten exons with introns ranging from 81-119 bp. Cathepsin W protein is expressed specifically in CD8+ T lymphocytes. The expression of cathepsin W first occurs during the differentiation of thyrocytes to CD8+ T lymphocytes, just as the thymocytes cease expression of CD4+ receptors. In transfected Cos-7 and HeLa cells, cathepsin W localizes within the rough endoplasmic reticulum. Cathepsin W contains a unique 21 amino acid peptide insertion between the active site histidine and asparagine residues, in addition to a distictive 8-amino acid carboxy-terminal extension. An extended loop struc-ture in the second or beta-sheet domain and an additional disulfide bind are two of several signature features of cathepsin W. Other features of cathepsin W include an additional cysteine, an S2 pocket and an additional residue. Cathepsin W may exist as a dimer with each monomer forming a disulfide bond.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cathepsin W; Lymphopain
Gene Aliases: CTSW; LYPN
UniProt ID: (Human) P56202
Entrez Gene ID: (Human) 1521
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