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Product Details
PHC1066
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Molecular weight
8 kDa
Class
Recombinant
Type
Protein
Purity
>95% by SDS-PAGE
Endotoxin concentration
<0.1 ng/µg
Activity
Determined by measuring the dose-dependent mobilization of intracellular calcium (calcium flux) with human neutrophils. Significant calcium mobilization was observed with ≥ 1 ng/mL of human CXCL1 (GRO-alpha).
Conjugate
Form
Lyophilized
Purification
purified
Contains
no preservative
Storage conditions
4° C
Shipping conditions
Wet ice
Product Specific Information
Carrier-Free
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute lyophilized human GRO-alpha in sterile, distilled water to 0.1-0.5 mg/mL. These stock solutions should be apportioned into working aliquots and stored at -20°C. Further dilution should be made in medium or buffered solution containing carrier protein, such as PBS with 0.1% BSA.
Storage: Lyophilized human GRO-alpha should be stored at 2°C to 8°C, preferably desiccated. Store reconstituted human GRO-alpha at -20°C (not in a frost-free freezer). Keep freeze-thaw cycles to a minimum.
Target Information
CXCL1, CXCL2, and CXCL3, also known respectively as GRO alpha, GRO beta (MIP-2 alpha) and GRO gamma (MIP-2 beta), are members of the CXC subfamily of chemokines. Mature CXCL1/2/3 proteins bind with high affinity to the IL-8 receptor type B and are potent neutrophil attractants and activators. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 1; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha); CXC; CXC motif chemokine; CXCL; CXCL-1; fibroblast secretory protein; GRO a; GRO α; GRO-alpha(1-73); GRO1 oncogene (melanoma growth stimulating activity, alpha); GRO1 oncogene (melanoma growth-stimulating activity); GROalpha; Growth-regulated alpha protein; GROα; melanoma growth stimulating activity, alpha; Melanoma growth stimulatory activity; melanoma growth stimulatory activity alpha; MGSA; MGSA alpha; NAP-3; NAP3; Neutrophil-activating protein 3
Gene Aliases: CXCL1; FSP; GRO; GRO1; GROA; MGSA; MGSA-a; NAP-3; SCYB1
UniProt ID: (Human) P09341
Entrez Gene ID: (Human) 2919
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