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Human CXCL12 (SDF-1b) Recombinant Protein, PeproTech®

Human CXCL12 (SDF-1b) Protein in Functional Assay (Functional)

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Human CXCL12 (SDF-1b) Protein (300-28B-10UG) in Functional

Bioassay analysis of Human CXCL12 (SDF-1b) Recombinant Protein, PeproTech® (Product # 300-28B-1MG).

Product Image Gallery

Product Details

300-28B-10UG

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

ELISA (ELISA)

Assay-dependent

View 1 publication 1 publication

Functional Assay (Functional)

Assay-dependent

In vitro Assay (IV)

View 16 publications 16 publications

Miscellaneous PubMed (Misc)

View 3 publications 3 publications

Product Specifications

Species

Human

Published species

Human, Non-human primate

Expression System

E. coli

Amino acid sequence

KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNKRF KM

Molecular weight

8.5 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

Determined by its ability to chemoattract activated human T-lymphocytes using a concentration range of 10-50 ng/ml.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Human SDF-1beta is an 8.5 kDa protein containing 72 amino acid residues.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

CXCL12 is a stromal cell-derived alpha chemokine member of the intercrine family. This gene product and its receptor CXCR4 can activate lymphocytes and have been implicated in the metastasis of some cancers such as breast cancer. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Chemokine Receptor Expression on T Cells Is Modulated by CAFs and Chemokines Affect the Spatial Distribution of T Cells in Pancreatic Tumors.

Cancers

Gorchs L,Oosthoek M,Yucel-Lindberg T,Moro CF,Kaipe H

300-28B was used in In vitro experiments to study how human cancer-associated fibroblasts (CAFs) modulate chemokine receptors which are known to be associated with T cell trafficking, CXCR3 and CCR5, and T cell exclusion, CXCR4.

Sat Aug 06 00:00:00 EDT 2022

Immunomodulatory effects of different intravenous immunoglobulin preparations in chronic lymphocytic leukemia.

Scientific reports

Colado A,Elías EE,Sarapura Martínez VJ,Cordini G,Morande P,Bezares F,Giordano M,Gamberale R,Borge M

Mon Jun 21 00:00:00 EDT 2021

Systems biology drug screening identifies statins as enhancers of current therapies in chronic lymphocytic leukemia.

Scientific reports

Gimenez N,Tripathi R,Giró A,Rosich L,López-Guerra M,López-Oreja I,Playa-Albinyana H,Arenas F,Mas JM,Pérez-Galán P,Delgado J,Campo E,Farrés J,Colomer D

Thu Dec 17 00:00:00 EST 2020

A knottin scaffold directs the CXC-chemokine-binding specificity of tick evasins.

The Journal of biological chemistry

Lee AW,Deruaz M,Lynch C,Davies G,Singh K,Alenazi Y,Eaton JRO,Kawamura A,Shaw J,Proudfoot AEI,Dias JM,Bhattacharya S

Fri Jul 19 00:00:00 EDT 2019

Genetically engineered two-warhead evasins provide a method to achieve precision targeting of disease-relevant chemokine subsets.

Scientific reports

Alenazi Y,Singh K,Davies G,Eaton JRO,Elders P,Kawamura A,Bhattacharya S

Fri Apr 20 00:00:00 EDT 2018

Yeast surface display identifies a family of evasins from ticks with novel polyvalent CC chemokine-binding activities.

Scientific reports

Singh K,Davies G,Alenazi Y,Eaton JRO,Kawamura A,Bhattacharya S

Tue Jun 27 00:00:00 EDT 2017

CXCR4, but not CXCR7, discriminates metastatic behavior in non-small cell lung cancer cells.

Molecular cancer research : MCR

Choi YH,Burdick MD,Strieter BA,Mehrad B,Strieter RM

300-28B was used in In vitro experiments to indicate that CXCR4, not CXCR7, expression engages CXCL12 to mediate NSCLC metastatic behavior.

Wed Jan 01 00:00:00 EST 2014

Hypoxia stimulates CXCR4 signalling in ileal carcinoids.

Endocrine-related cancer

Arvidsson Y,Bergström A,Arvidsson L,Kristiansson E,Ahlman H,Nilsson O

300-28B was used in Cell Culture to show that targeting of HIFs and/or the CXCR4 signalling pathway may offer new therapeutic strategies for carcinoid tumour disease.

Tue Jun 01 00:00:00 EDT 2010

Carboxypeptidase M expressed by human bone marrow cells cleaves the C-terminal lysine of stromal cell-derived factor-1alpha: another player in hematopoietic stem/progenitor cell mobilization?

Stem cells (Dayton, Ohio)

Marquez-Curtis L,Jalili A,Deiteren K,Shirvaikar N,Lambeir AM,Janowska-Wieczorek A

Thu May 01 00:00:00 EDT 2008

A chemokine-binding domain in the tumor necrosis factor receptor from variola (smallpox) virus.

Proceedings of the National Academy of Sciences of the United States of America

Alejo A,Ruiz-Argüello MB,Ho Y,Smith VP,Saraiva M,Alcami A

Tue Apr 11 00:00:00 EDT 2006

Structure/function relationships of CCR8 agonists and antagonists. Amino-terminal extension of CCL1 by a single amino acid generates a partial agonist.

The Journal of biological chemistry

Fox JM,Najarro P,Smith GL,Struyf S,Proost P,Pease JE

Fri Dec 01 00:00:00 EST 2006

Involvement of the urokinase-type plasminogen activator receptor in hematopoietic stem cell mobilization.

Blood

Selleri C,Montuori N,Ricci P,Visconte V,Carriero MV,Sidenius N,Serio B,Blasi F,Rotoli B,Rossi G,Ragno P

Tue Mar 01 00:00:00 EST 2005

Neuropilin-1 regulates attachment in human endothelial cells independently of vascular endothelial growth factor receptor-2.

Blood

Murga M,Fernandez-Capetillo O,Tosato G

Tue Mar 01 00:00:00 EST 2005

Role of ChemR23 in directing the migration of myeloid and plasmacytoid dendritic cells to lymphoid organs and inflamed skin.

The Journal of experimental medicine

Vermi W,Riboldi E,Wittamer V,Gentili F,Luini W,Marrelli S,Vecchi A,Franssen JD,Communi D,Massardi L,Sironi M,Mantovani A,Parmentier M,Facchetti F,Sozzani S

Mon Feb 21 00:00:00 EST 2005

Molecular mechanism of AMD3100 antagonism in the CXCR4 receptor: transfer of binding site to the CXCR3 receptor.

The Journal of biological chemistry

Rosenkilde MM,Gerlach LO,Jakobsen JS,Skerlj RT,Bridger GJ,Schwartz TW

Fri Jan 23 00:00:00 EST 2004

The CXC chemokine receptor encoded by herpesvirus saimiri, ECRF3, shows ligand-regulated signaling through Gi, Gq, and G12/13 proteins but constitutive signaling only through Gi and G12/13 proteins.

The Journal of biological chemistry

Rosenkilde MM,McLean KA,Holst PJ,Schwartz TW

Fri Jul 30 00:00:00 EDT 2004

Human P-selectin glycoprotein ligand-1 (PSGL-1) interacts with the skin-associated chemokine CCL27 via sulfated tyrosines at the PSGL-1 amino terminus.

The Journal of biological chemistry

Hirata T,Furukawa Y,Yang BG,Hieshima K,Fukuda M,Kannagi R,Yoshie O,Miyasaka M

Fri Dec 10 00:00:00 EST 2004

Role of tyrosine phosphorylation in ligand-independent sequestration of CXCR4 in human primary monocytes-macrophages.

The Journal of biological chemistry

Wang J,Guan E,Roderiquez G,Calvert V,Alvarez R,Norcross MA

Fri Dec 28 00:00:00 EST 2001

CD40-Mediated induction of CD4 and CXCR4 on B lymphocytes correlates with restricted susceptibility to human immunodeficiency virus type 1 infection: potential role of B lymphocytes as a viral reservoir.

Journal of virology

Moir S,Lapointe R,Malaspina A,Ostrowski M,Cole CE,Chun TW,Adelsberger J,Baseler M,Hwu P,Fauci AS

Fri Oct 01 00:00:00 EDT 1999

Chemokines and the arrest of lymphocytes rolling under flow conditions.

Science (New York, N.Y.)

Campbell JJ,Hedrick J,Zlotnik A,Siani MA,Thompson DA,Butcher EC

Fri Jan 16 00:00:00 EST 1998

Bioinformatics

Protein Aliases: C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 12; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 12; CXC; CXC motif chemokine; CXCL; Intercrine reduced in hepatomas; IRH; PBSF; Pre-B cell growth-stimulating factor; SDF-1; sdf-1 beta; Stromal cell-derived factor 1

Human CXCL12 (SDF-1b) Recombinant Protein, PeproTech®

Human CXCL12 (SDF-1b) Protein (300-28B-10UG) in Functional

Product Details

300-28B-10UG

Western Blot (WB)

ELISA (ELISA)

Functional Assay (Functional)

In vitro Assay (IV)

Miscellaneous PubMed (Misc)

Species

Published species

Expression System

Amino acid sequence

Molecular weight

Class

Type

Purity

Endotoxin concentration

Activity

Conjugate

Form

Purification

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

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