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Invitrogen
Human CYB561D2 (aa 1-21) Control Fragment Recombinant Protein
Product Details
RP-100132
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
MALSAETESHIYRALRTASGA
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
PBS/1M urea, pH 7.4
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (95%), Rat (95%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-60137. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
CYB561D2 (Cytochrome b561 domain-containing protein 2), also known as putative tumor suppressor protein 101F6, is a 222 amino acid highly hydrophobic protein that contains six membrane spanning domains. Localized to endosomes and endoplasmic reticulum of the perinuclear region, CYB561D2 contains 2 heme-binding sites, through which it reduces ferric ions. CYB561D2 is ubiquitously expressed, with highest levels in lung, liver and kidney. Though expression of CYB561D2 is abundant in normal lung bronchial epithileal cells, CYB561D2 is absent in most lung cancers. The gene encoding CYB561D2 maps to human chromosome 3p21.3, a region that is prone to genetic alterations and is associated with frequent and early allele loss in many human cancers. Exogenous expression of CYB561D2 leads to the induction of caspase-independent apoptotic and autophagic pathways, resulting in the synergistic killing of tumor cells. This suggests an important role for CYB561D2 as a therapeutic intervention in cancer treatment.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cytochrome b561 domain-containing protein 2; cytochrome b561 family, member D2; OTTHUMP0000021067; OTTHUMP00000210675; OTTHUMP00000210676; Putative tumor suppressor protein 101F6; Transmembrane reductase CYB561D2
Gene Aliases: 101F6; CYB561D2; LUCA12.2; TSP10; XXcos-LUCA11.4
UniProt ID: (Human) O14569
Entrez Gene ID: (Human) 11068
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