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Invitrogen
Human Cardiac Troponin T (aa 170-286) Control Fragment Recombinant Protein
Product Details
RP-91307
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
EEEENRRKAEDEARKKKALSNMMHFGGYIQKQAQTERKSGKRQTEREKKKKILAERRKVLAIDHLNEDQLREKAKELWQSIYNLEAEKFDLQEKFKQQKYEINVLRNRINDNQKVSK
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
PBS/1M urea, pH 7.4
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (98%), Rat (98%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-82539. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
Cardiac Troponin T is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Cardiac Troponin T is important in proper muscle function and myofibril formation. In the human heart, four cTnT isoforms are expressed in a developmentally regulated manner. Cardiac Troponin T expression in the human heart is affected by heart failure. Mutations within the Cardiac Troponin T protein have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for the Cardiac Troponin T gene undergo alternative splicing that results in many tissue-specific isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cardiac muscle troponin T; cardiomyopathy, dilated 1D (autosomal dominant); cardiomyopathy, hypertrophic 2; cTnT; MGC3889; TnTc; troponin T type 2 (cardiac); Troponin T, cardiac muscle; Troponin T2; troponin T2, cardiac; truncated cardiac troponin T
Gene Aliases: CMD1D; CMH2; CMPD2; cTnT; LVNC6; RCM3; TNNT2; TnTC
UniProt ID: (Human) P45379
Entrez Gene ID: (Human) 7139
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