Gibco

Human Eotaxin-3 (CCL26) Recombinant Protein, PeproTech®

Human Eotaxin-3 (CCL26) Protein in Functional Assay (Functional)

FIGURE: 1 / 1

Human Eotaxin-3 (CCL26) Protein (300-48-20UG) in Functional

Bioassay analysis of Human Eotaxin-3 (CCL26) Recombinant Protein, PeproTech® (Product # 300-48-1MG).

Product Image Gallery

Product Details

300-48-20UG

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

Functional Assay (Functional)

Assay-dependent

In vitro Assay (IV)

View 8 publications 8 publications

Miscellaneous PubMed (Misc)

View 4 publications 4 publications

Product Specifications

Species

Human

Published species

Bacteria, Human, Mouse

Expression System

E. coli

Amino acid sequence

TRGSDISKTC CFQYSHKPLP WTWVRSYEFT SNSCSQRAVI FTTKRGKKVC THPRKKWVQK YISLLKTPKQ L

Molecular weight

8.4 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

Determined by its ability to chemoattract human CCR3/HEK293 cells. The maximum activity was achieved at 2ug/ml.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Human Eotaxin-3 is an 8.4 kDa protein containing 71 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

Eotaxin-3 is a small cytokine belonging to the CC chemokine family that is also called CCL26, Chemokine (C-C motif) ligand 26. Chemokines play pivotal roles in health and disease by controlling migration and tissue localization of specific types of cells expressing their cognate receptors. Eotaxin-3 is chemotactic for eosinophils and basophils and elicits its effects by binding to the cell surface chemokine receptor CCR3. It is expressed by several tissues including heart, lung and ovary, and in endothelial cells that have been stimulated with the cytokine interleukin 4. This gene for chemokine is located on human chromosome 7. Eotaxin-3 induced migration of eosinophils and basophils only at a 10-fold higher concentration than eotaxin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Bidirectional crosstalk between eosinophils and esophageal epithelial cells regulates inflammatory and remodeling processes.

Mucosal immunology

Dunn JLM,Caldwell JM,Ballaban A,Ben-Baruch Morgenstern N,Rochman M,Rothenberg ME

300-48 was used in Sample Preparation (FC/FACS) to study the bidirectional communication between the eosinophils which accumulate adjacent to epithelial cells in the mucosa of patients with eosinophilic esophagitis (EoE) to uncover a network of crosstalk between eosinophils and esophageal epithelial cells involving epithelial mediated eosinophil survival and reciprocal changes in cellular transcripts, events likely to occur in EoE.

Wed Sep 01 00:00:00 EDT 2021

A knottin scaffold directs the CXC-chemokine-binding specificity of tick evasins.

The Journal of biological chemistry

Lee AW,Deruaz M,Lynch C,Davies G,Singh K,Alenazi Y,Eaton JRO,Kawamura A,Shaw J,Proudfoot AEI,Dias JM,Bhattacharya S

Fri Jul 19 00:00:00 EDT 2019

Genetically engineered two-warhead evasins provide a method to achieve precision targeting of disease-relevant chemokine subsets.

Scientific reports

Alenazi Y,Singh K,Davies G,Eaton JRO,Elders P,Kawamura A,Bhattacharya S

Fri Apr 20 00:00:00 EDT 2018

Yeast surface display identifies a family of evasins from ticks with novel polyvalent CC chemokine-binding activities.

Scientific reports

Singh K,Davies G,Alenazi Y,Eaton JRO,Kawamura A,Bhattacharya S

Tue Jun 27 00:00:00 EDT 2017

Atypical chemokine receptor ACKR2 mediates chemokine scavenging by primary human trophoblasts and can regulate fetal growth, placental structure, and neonatal mortality in mice.

Journal of immunology (Baltimore, Md. : 1950)

Teoh PJ,Menzies FM,Hansell CA,Clarke M,Waddell C,Burton GJ,Nelson SM,Nibbs RJ

Sat Nov 15 00:00:00 EST 2014

IL-4 engagement of the type I IL-4 receptor complex enhances mouse eosinophil migration to eotaxin-1 in vitro.

PloS one

Heller NM,Gwinn WM,Donnelly RP,Constant SL,Keegan AD

Fri Jan 04 00:00:00 EST 2013

Host chemokines bind to Staphylococcus aureus and stimulate protein A release.

The Journal of biological chemistry

Yung SC,Parenti D,Murphy PM

Fri Feb 18 00:00:00 EST 2011

Eotaxin-3/CC chemokine ligand 26 is a functional ligand for CX3CR1.

Journal of immunology (Baltimore, Md. : 1950)

Nakayama T,Watanabe Y,Oiso N,Higuchi T,Shigeta A,Mizuguchi N,Katou F,Hashimoto K,Kawada A,Yoshie O

Wed Dec 01 00:00:00 EST 2010

Eotaxin-3/CC chemokine ligand 26 is a functional ligand for CX3CR1.

Journal of immunology (Baltimore, Md. : 1950)

Nakayama T,Watanabe Y,Oiso N,Higuchi T,Shigeta A,Mizuguchi N,Katou F,Hashimoto K,Kawada A,Yoshie O

Wed Dec 01 00:00:00 EST 2010

Role of the CXCR4/CXCL12 axis in lymphangioleiomyomatosis and angiomyolipoma.

Journal of immunology (Baltimore, Md. : 1950)

Clements D,Markwick LJ,Puri N,Johnson SR

Sun Aug 01 00:00:00 EDT 2010

CCR3 is a target for age-related macular degeneration diagnosis and therapy.

Nature

Takeda A,Baffi JZ,Kleinman ME,Cho WG,Nozaki M,Yamada K,Kaneko H,Albuquerque RJ,Dridi S,Saito K,Raisler BJ,Budd SJ,Geisen P,Munitz A,Ambati BK,Green MG,Ishibashi T,Wright JD,Humbles AA,Gerard CJ,Ogura Y,Pan Y,Smith JR,Grisanti S,Hartnett ME,Rothenberg ME,Ambati J

Thu Jul 09 00:00:00 EDT 2009

Citrullination of CXCL8 by peptidylarginine deiminase alters receptor usage, prevents proteolysis, and dampens tissue inflammation.

The Journal of experimental medicine

Proost P,Loos T,Mortier A,Schutyser E,Gouwy M,Noppen S,Dillen C,Ronsse I,Conings R,Struyf S,Opdenakker G,Maudgal PC,Van Damme J

Mon Sep 01 00:00:00 EDT 2008

Structure and function of A41, a vaccinia virus chemokine binding protein.

PLoS pathogens

Bahar MW,Kenyon JC,Putz MM,Abrescia NG,Pease JE,Wise EL,Stuart DI,Smith GL,Grimes JM

Tue Jan 01 00:00:00 EST 2008

Bioinformatics

Protein Aliases: C-C motif chemokine 26; CC chemokine IMAC; chemokine (C-C motif) ligand 26; chemokine N1; Eotaxin-3; Eotaxin3; Macrophage inflammatory protein 4-alpha; MGC126714; MIP-4-alpha; PRO242; SCYA26MIP-4-alpha; small inducible cytokine A26; small inducible cytokine subfamily A (Cys-Cys), member 26; Small-inducible cytokine A26; Thymic stroma chemokine-1; TSC-1; UNQ216

Human Eotaxin-3 (CCL26) Recombinant Protein, PeproTech®

Human Eotaxin-3 (CCL26) Protein (300-48-20UG) in Functional

Product Details

300-48-20UG

Western Blot (WB)

ELISA (ELISA)

Functional Assay (Functional)

In vitro Assay (IV)

Miscellaneous PubMed (Misc)

Species

Published species

Expression System

Amino acid sequence

Molecular weight

Class

Type

Purity

Endotoxin concentration

Activity

Conjugate

Form

Purification

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

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