Gibco

Human FGF-19 Recombinant Protein, PeproTech®

Product Details

100-32-25UG

Applications

Tested Dilution

Publications

Functional Assay (Functional)

Assay-dependent

In vitro Assay (IV)

View 12 publications 12 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species

Human

Published species

Chicken, Hamster, Human, Mouse

Expression System

E. coli

Amino acid sequence

MRPLAFSDAG PHVHYGWGDP IRLRHLYTSG PHGLSSCFLR IRADGVVDCA RGQSAHSLLE IKAVALRTVA IKGVHSVRYL CMGADGKMQG LLQYSEEDCA FEEEIRPDGY NVYRSEKHRL PVSLSSAKQR QLYKNRGFLP LSHFLPMLPM VPEEPEDLRG HLESDMFSSP LETDSMDPFG LVTGLEAVRS PSFEK

Molecular weight

21.8 kDa

Class

Recombinant

Type

Protein

Purity

≥ 95% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

Determined by a cell proliferation assay using balb/c 3T3 cells. The expected ED50 for this effect is 100-150 ng/ml.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

100-32-1MG will be provided as 2 x 500 µg (100-32-500UG).

Recombinant Human FGF-19 is a 21.8 kDa protein containing 195 amino acid residues.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

FGF19, Fibroblast growth factor 19, is a protein that in humans is encoded by the FGF19 gene. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. The FGF19 gene is mapped to 11q13.3. The deduced 216-amino acid FGF19 protein contains asignal sequence and 2 cysteine residues that are conserved in the FGF family. Expression of this gene was detected only in fetal but not adult brain tissue. Synergistic interaction of the chick homolog and Wnt-8c has been shown to be required for initiation of inner ear development. FGF19 stimulates hepatic protein and glycogen synthesis but does not induce lipogenesis. The effects of FGF19 are independent of the activity of either insulin or the protein kinase Akt and, instead, are mediated through a mitogen-activated protein kinase signaling pathway that activates components of the protein translation machinery and stimulates glycogen synthase activity. The orthologous protein in mouse is FGF15, which shares about 50% amino acid identity and has similar functions. Together they are often referred to as FGF15/19.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Generation of multilineage liver organoids with luminal vasculature and bile ducts from human pluripotent stem cells via modulation of Notch signaling.

Stem cell research & therapy

Kim HJ,Kim G,Chi KY,Kim H,Jang YJ,Jo S,Lee J,Lee Y,Woo DH,Han C,Kim SK,Park HJ,Kim JH

100-32 was used in Cell Culture to demonstrate that mLOs can reproduce parenchymal and non-parenchymal cell interactions and suggest that their application can advance the precise modeling of liver diseases in vitro.

Fri Feb 03 00:00:00 EST 2023

Recapitulating hepatitis E virus-host interactions and facilitating antiviral drug discovery in human liver-derived organoids.

Science advances

Li P,Li Y,Wang Y,Liu J,Lavrijsen M,Li Y,Zhang R,Verstegen MMA,Wang Y,Li TC,Ma Z,Kainov DE,Bruno MJ,de Man RA,van der Laan LJW,Peppelenbosch MP,Pan Q

Fri Jan 21 00:00:00 EST 2022

Generation, Maintenance and HBV Infection of Human Liver Organoids.

Bio-protocol

Romal S,Hossain T,Mahmoudi T

100-32 was used in Cell Culture to propose the use of human liver organoids as a platform for modeling HBV infection and related tumorigenesis.

Sun Mar 20 00:00:00 EDT 2022

FGF7-FGFR2 autocrine signaling increases growth and chemoresistance of fusion-positive rhabdomyosarcomas.

Molecular oncology

Milton CI,Selfe J,Aladowicz E,Man SYK,Bernauer C,Missiaglia E,Walters ZS,Gatz SA,Kelsey A,Generali M,Box G,Valenti M,de Haven-Brandon A,Galiwango D,Hayes A,Clarke M,Izquierdo E,Gonzalez De Castro D,Raynaud FI,Kirkin V,Shipley JM

100-32 was used in Cell Culture to investigate the effects of Fibroblast growth factor receptor inhibitors to rhabdomyosarcoma progression.

Tue Mar 01 00:00:00 EST 2022

Clinical stage drugs targeting inhibitor of apoptosis proteins purge episomal Hepatitis B viral genome in preclinical models.

Cell death & disease

Clark MP,Huynh T,Rao S,Mackiewicz L,Mason H,Romal S,Stutz MD,Ahn SH,Earnest L,Sozzi V,Littlejohn M,Tran BM,Wiedemann N,Vincan E,Torresi J,Netter HJ,Mahmoudi T,Revill P,Pellegrini M,Ebert G

Wed Jun 23 00:00:00 EDT 2021

The impact of FGF19/FGFR4 signaling inhibition in antitumor activity of multi-kinase inhibitors in hepatocellular carcinoma.

Scientific reports

Kanzaki H,Chiba T,Ao J,Koroki K,Kanayama K,Maruta S,Maeda T,Kusakabe Y,Kobayashi K,Kanogawa N,Kiyono S,Nakamura M,Kondo T,Saito T,Nakagawa R,Ogasawara S,Suzuki E,Ooka Y,Muroyama R,Nakamoto S,Yasui S,Tawada A,Arai M,Kanda T,Maruyama H,Mimura N,Kato J,Zen Y,Ohtsuka M,Iwama A,Kato N

Fri Mar 05 00:00:00 EST 2021

Gene manipulation in liver ductal organoids by optimized recombinant adeno-associated virus vectors.

The Journal of biological chemistry

Wei J,Ran G,Wang X,Jiang N,Liang J,Lin X,Ling C,Zhao B

Fri Sep 20 00:00:00 EDT 2019

Fibroblast growth factor 8b induces uncoupling protein 1 expression in epididymal white preadipocytes.

Scientific reports

Westphal S,Gantert T,Kless C,Hüttinger K,Klingenspor M,Fromme T

Tue Jun 11 00:00:00 EDT 2019

A computationally identified compound antagonizes excess FGF-23 signaling in renal tubules and a mouse model of hypophosphatemia.

Science signaling

Xiao Z,Riccardi D,Velazquez HA,Chin AL,Yates CR,Carrick JD,Smith JC,Baudry J,Quarles LD

Tue Nov 22 00:00:00 EST 2016

Fibroblast growth factor receptor 4 promotes progression and correlates to poor prognosis in cholangiocarcinoma.

Biochemical and biophysical research communications

Xu YF,Yang XQ,Lu XF,Guo S,Liu Y,Iqbal M,Ning SL,Yang H,Suo N,Chen YX

Fri Mar 28 00:00:00 EDT 2014

Fibroblast growth factor and bone morphogenetic protein signaling are required for specifying prechondrogenic identity in neural crest-derived mesenchyme and initiating the chondrogenic program.

Developmental dynamics : an official publication of the American Association of Anatomists

Kumar M,Ray P,Chapman SC

Fri Jun 01 00:00:00 EDT 2012

Relevant use of Klotho in FGF19 subfamily signaling system in vivo.

Proceedings of the National Academy of Sciences of the United States of America

Tomiyama K,Maeda R,Urakawa I,Yamazaki Y,Tanaka T,Ito S,Nabeshima Y,Tomita T,Odori S,Hosoda K,Nakao K,Imura A,Nabeshima Y

Tue Jan 26 00:00:00 EST 2010

FGF19 is a target for FOXC1 regulation in ciliary body-derived cells.

Human molecular genetics

Tamimi Y,Skarie JM,Footz T,Berry FB,Link BA,Walter MA

Wed Nov 01 00:00:00 EST 2006

Bioinformatics

Protein Aliases: FGF; FGF-19; Fibroblast growth factor; Fibroblast growth factor 19