Gibco

Human FGF-4, Animal-Free Recombinant Protein, PeproTech®

Product Details

AF-100-31-25UG

Applications

Tested Dilution

Publications

Functional Assay (Functional)

Assay-dependent

In vitro Assay (IV)

View 9 publications 9 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species

Human

Published species

Human, Mouse, Pig

Expression System

E. coli

Amino acid sequence

GRGGAAAPTA PNGTLEAELE RRWESLVALS LARLPVAAQP KEAAVQSGAG DYLLGIKRLR RLYCNVGIGF HLQALPDGRI GGAHADTRDS LLELSPVERG VVSIFGVASR FFVAMSSKGK LYGSPFFTDE CTFKEILLPN NYNAYESYKY PGMFIALSKN GKTKKGNRVS PTMKVTHFLP RL

Molecular weight

19.7 kDa

Class

Recombinant

Type

Protein

Purity

≥ 95% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<0.1 EU/µg

Activity

Determined by a cell proliferation assay using Balb/c 3T3 cells. The expected ED50 is ≤ 10 ng/ml, corresponding to a specific activity of ≥ 1 x 10^5 units/mg.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

AF-100-31-1MG will be provided as 2 x 500 µg (AF-100-31-500UG).

Recombinant Human FGF-4 is a 19.7 kDa protein consisting of 182 amino acid residues.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

Fibroblast growth factor 4 (FGF4) is a member of the fibroblast growth factor (FGF) family that possess broad mitogenic and cell survival activities and play key roles in growth and survival of stem cells during embryogenesis, tissue regeneration, and carcinogenesis. FGF4 was identified by its strong oncogenic transforming activity and is a potent angiogenic factor, expressed in several highly vascularized tumors and also in adult mouse testis, intestine, and brain. Studies on the mouse homolog suggests a function in bone morphogenesis and limb development through the sonic hedgehog (SHH) signaling pathway. Furthermore, FGF4 regulates neural progenitor cell proliferation and neuronal differentiation. Recent studies show a growth-promoting role for FGF4 in human embryonic stem cells and a putative feedback inhibition mechanism by a novel FGF4 splice isoform that may serve to promote differentiation at a later stages of development.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Acute liver steatosis translationally controls the epigenetic regulator MIER1 to promote liver regeneration in a study with male mice.

Nature communications

Chen Y,Chen L,Wu X,Zhao Y,Wang Y,Jiang D,Liu X,Zhou T,Li S,Wei Y,Liu Y,Hu C,Zhou B,Qin J,Ying H,Ding Q

AF-100-31 was used in In vitro experiments to identify an epigenetic mechanism by which the early phase lipid redistribution from adipose tissue to liver during regeneration impacts hepatocyte proliferation, and suggest a potential strategy to boost liver regeneration.

Sat Mar 18 00:00:00 EDT 2023

Rif1 and Hmgn3 regulate the conversion of murine trophoblast stem cells.

Cell reports

Zhang W,Yao C,Luo Y,Li Q,Zhao Q,Zhao Y,Wang Y,Geng M,Wang Q,Xu M,Sun S,Wu D,Gao Q,Wu X,Shuai L

AF-100-31 was used in Sample Preparation to study the mechanisms underlying trophectoderm (TE) specification, a hallmark event in preimplantation development during murine embryogenesis, to report two key genes regulating the conversion of trophoblast stem cells (TSCs) and provide insights for investigating TE specification.

Tue Mar 29 00:00:00 EDT 2022

An airway organoid-based screen identifies a role for the HIF1α-glycolysis axis in SARS-CoV-2 infection.

Cell reports

Duan X,Tang X,Nair MS,Zhang T,Qiu Y,Zhang W,Wang P,Huang Y,Xiang J,Wang H,Schwartz RE,Ho DD,Evans T,Chen S

Tue Nov 09 00:00:00 EST 2021

Phosphoproteomics identifies a bimodal EPHA2 receptor switch that promotes embryonic stem cell differentiation.

Nature communications

Fernandez-Alonso R,Bustos F,Budzyk M,Kumar P,Helbig AO,Hukelmann J,Lamond AI,Lanner F,Zhou H,Petsalaki E,Findlay GM

Fri Mar 13 00:00:00 EDT 2020

Mapping the Mouse Cell Atlas by Microwell-Seq.

Cell

Han X,Wang R,Zhou Y,Fei L,Sun H,Lai S,Saadatpour A,Zhou Z,Chen H,Ye F,Huang D,Xu Y,Huang W,Jiang M,Jiang X,Mao J,Chen Y,Lu C,Xie J,Fang Q,Wang Y,Yue R,Li T,Huang H,Orkin SH,Yuan GC,Chen M,Guo G

Thu Feb 22 00:00:00 EST 2018

Mapping the Mouse Cell Atlas by Microwell-Seq.

Cell

Han X,Wang R,Zhou Y,Fei L,Sun H,Lai S,Saadatpour A,Zhou Z,Chen H,Ye F,Huang D,Xu Y,Huang W,Jiang M,Jiang X,Mao J,Chen Y,Lu C,Xie J,Fang Q,Wang Y,Yue R,Li T,Huang H,Orkin SH,Yuan GC,Chen M,Guo G

Thu Feb 22 00:00:00 EST 2018

Expression of endogenous retroviruses is negatively regulated by the pluripotency marker Rex1/Zfp42.

Nucleic acids research

Guallar D,Pérez-Palacios R,Climent M,Martínez-Abadía I,Larraga A,Fernández-Juan M,Vallejo C,Muniesa P,Schoorlemmer J

Mon Oct 01 00:00:00 EDT 2012

Modulation of pluripotency in the porcine embryo and iPS cells.

PloS one

Rodríguez A,Allegrucci C,Alberio R

Thu May 23 00:00:00 EDT 2013

Ontological differences in first compared to third trimester human fetal placental chorionic stem cells.

PloS one

Jones GN,Moschidou D,Puga-Iglesias TI,Kuleszewicz K,Vanleene M,Shefelbine SJ,Bou-Gharios G,Fisk NM,David AL,De Coppi P,Guillot PV

Tue Jan 22 00:00:00 EST 2013

Therapeutic potential of adipose tissue-derived stem cells for liver failure according to the transplantation routes.

Journal of the Korean Surgical Society

Kim SJ,Park KC,Lee JU,Kim KJ,Kim DG

Thu Sep 01 00:00:00 EDT 2011

Efficient generation of hepatocyte-like cells from human induced pluripotent stem cells.

Cell research

Song Z,Cai J,Liu Y,Zhao D,Yong J,Duo S,Song X,Guo Y,Zhao Y,Qin H,Yin X,Wu C,Che J,Lu S,Ding M,Deng H

Sun Nov 01 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: FGF; FGF-4; Fibroblast growth factor; Fibroblast growth factor 4; fibroblast growth factor 4 splice isoform; HBGF-4; heparin secretory transforming protein 1; Heparin secretory-transforming protein 1; Heparin-binding growth factor 4; HST; HSTF-1; human stomach cancer, transforming factor from FGF-related oncogene; kaposi sarcoma oncogene; oncogene HST; Transforming protein KS3