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Product Details
PHG0094
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
Human FGF-7 (KGF) recombinant protein contains 163 amino acids
Molecular weight
18.9 kDa
Class
Recombinant
Type
Protein
Purity
≥95% by SDS-PAGE and HPLC
Endotoxin concentration
<0.1 ng/µg
Activity
ED50 = 0.1 - 15 ng/mL; determined by the dose-dependent proliferation of 4MBr-5 cells.
Conjugate
Form
Lyophilized
Purification
purified
Storage buffer
PBS, pH 7, with 0.25M NaCl
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Wet ice (domestic); Wet or Dry ice (international)
Product Specific Information
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute KGF in 1X PBS to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2°C to 8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein, such as 0.1% BSA and store in working aliquots at -20°C to -80°C.
Storage: The lyophilized protein is stable at room temperature for 1 month, but should be kept at -20°C for long term storage, preferably desiccated. Working aliquots stored with a carrier protein are stable for at least 3 months at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
Target Information
Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. Required for normal branching morphogenesis. Growth factor active on keratinocytes. Possible major paracrine effector of normal epithelial cell proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FGF-7; Fibroblast growth factor 7; HBGF-7; Heparin-binding growth factor 7; Keratinocyte growth factor
Gene Aliases: FGF7; HBGF-7; KGF
UniProt ID: (Human) P21781
Entrez Gene ID: (Human) 2252
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