Gibco

Human GDNF Recombinant Protein, PeproTech®

Human GDNF Protein in Functional Assay (Functional)

FIGURE: 1 / 2

Human GDNF Protein (450-10-10UG) in Functional

Bioassay analysis of Human GDNF Recombinant Protein, PeproTech® (Product # 450-10-1MG).

Product Image Gallery

Product Details

450-10-10UG

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

Functional Assay (Functional)

Assay-dependent

View 1 publication 1 publication

In vitro Assay (IV)

View 271 publications 271 publications

Miscellaneous PubMed (Misc)

View 49 publications 49 publications

Product Specifications

Species

Human

Published species

Amphibian, Avian, Hamster, Human, Mouse, Non-human primate, Rat, Virus, Xenopus

Expression System

E. coli

Amino acid sequence

MSPDKQMAVL PRRERNRQAA AANPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCDAAETTY DKILKNLSRN RRLVSDKVGQ ACCRPIAFDD DLSFLDDNLV YHILRKHSAK RCGCI

Molecular weight

30.4 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

Determined by a cell proliferation assay using SH-SY5Y cells. The expected ED50 for this effect is 1.0-10.0 ng/ml.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

The functional human GDNF ligand is a disulfide-linked homodimer consisting of two 15 kDa polypeptide chains called monomers. Each monomer contains seven conserved cysteine residues, including Cys-101, which is used for inter-chain disulfide bridging, and others that are involved in the intramolecular ring formation known as the cysteine-knot configuration. The calculated molecular weight of Recombinant Human GDNF is 30.4 kDa.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

GDNF is a glycosylated, disulfide-bonded homodimer molecule. It was first discovered as a potent survival factor for midbrain dopaminergic neurons and was then shown to rescue these neurons in animal models of Parkinson's disease. GDNF is about 100 times more efficient survival factor for spinal motor neurons than the neurotrophins. GDNF is a neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Deterministic nuclear reprogramming of mammalian nuclei to a totipotency-like state by Amphibian meiotic oocytes for stem cell therapy in humans.

Biology open

Wen MH,Barbosa Triana H,Butler R,Hu HW,Dai YH,Lawrence N,Hong JJ,Garrett N,Jones-Green R,Rawlins EL,Dong Z,Koziol MJ,Gurdon JB

450-10 was used in Cell Culture to conclude that much of the reprogramming effect of eggs is already present in meiotic oocytes and does not require cell division or selection of dividing cells.

Fri Mar 01 00:00:00 EST 2024

Axin2 coupled excessive Wnt-glycolysis signaling mediates social defect in autism spectrum disorders.

EMBO molecular medicine

Wang M,Xian P,Zheng W,Li Z,Chen A,Xiao H,Xu C,Wang F,Mao H,Meng H,Zhao Y,Luo C,Wang Y,Wu S

450-10 was used in In vitro experiments to reveal excessive neuronal Wnt-glycolysis signaling as an important underlying mechanism for ASD synaptic deficiency, indicating Axin2 as a potential therapeutic target for social dysfunction.

Wed Jun 07 00:00:00 EDT 2023

Efficient generation of functional neurons from mouse embryonic stem cells via neurogenin-2 expression.

Nature protocols

Liu Y,Wang J,Südhof TC,Wernig M

450-10 was used in Cell Culture to provide a detailed approach for the efficient and reproducible generation of functional iN cells from mouse ESC cultures by the genetically induced expression of neurogenin-2.

Sun Oct 01 00:00:00 EDT 2023

METTL3-Dependent N6-Methyladenosine Modification Programs Human Neural Progenitor Cell Proliferation.

International journal of molecular sciences

Zhao Y,Li J,Lian Y,Zhou Q,Wu Y,Kang J

450-10 was used in Cell Culture to reveal a novel functional target (SLIT2) for METTL3 in hNPCs and contribute to a better understanding of m6A-dependent mechanisms in hNPC proliferation.

Tue Oct 24 00:00:00 EDT 2023

Regulation of synaptic connectivity in schizophrenia spectrum by mutual neuron-microglia interaction.

Communications biology

Breitmeyer R,Vogel S,Heider J,Hartmann SM,Wüst R,Keller AL,Binner A,Fitzgerald JC,Fallgatter AJ,Volkmer H

450-10 was used in In vitro experiments to present a co-culture model of neurons and microglia, both of human origin, to show increased susceptibility of neurons to microglia-like cells derived from SCZ patients.

Sat Apr 29 00:00:00 EDT 2023

Convergent coexpression of autism-associated genes suggests some novel risk genes may not be detectable in large-scale genetic studies.

Cell genomics

Liao C,Moyses-Oliveira M,De Esch CEF,Bhavsar R,Nuttle X,Li A,Yu A,Burt ND,Erdin S,Fu JM,Wang M,Morley T,Han L,Dion PA,Rouleau GA,Zhang B,Brennand KJ,Talkowski ME,Ruderfer DM

450-10 was used in Cell Culture to indicate that convergent coexpression can identify potentially novel genes that are unlikely to be discovered by sequencing studies.

Wed Apr 12 00:00:00 EDT 2023

Transcription Factor-Mediated Differentiation of Motor Neurons from Human Pluripotent Stem Cells.

Methods in molecular biology (Clifton, N.J.)

Wu J,Tang Y

450-10 was used in Cell Culture to describe a method of generating functional MNs via lentiviral delivery of transcription factors, based on the preservable NPC platform derived from hPSCs.

Thu Dec 15 00:00:00 EST 2022

An engineered Sox17 induces somatic to neural stem cell fate transitions independently from pluripotency reprogramming.

Science advances

Weng M,Hu H,Graus MS,Tan DS,Gao Y,Ren S,Ho DHH,Langer J,Holzner M,Huang Y,Ling GS,Lai CSW,Francois M,Jauch R

450-10 was used in In vitro experiments to report iNSC reprogramming from embryonic and aged mouse fibroblasts as well as from human blood using an engineered Sox17 (eSox17FNV).

Fri Aug 25 00:00:00 EDT 2023

Generation of locus coeruleus norepinephrine neurons from human pluripotent stem cells.

Nature biotechnology

Tao Y,Li X,Dong Q,Kong L,Petersen AJ,Yan Y,Xu K,Zima S,Li Y,Schmidt DK,Ayala M,Mathivanan S,Sousa AMM,Chang Q,Zhang SC

450-10 was used in Cell Culture to develop a method to generate 40-60% human LC-NE neurons from human pluripotent stem cells.

Thu Nov 16 00:00:00 EST 2023

Generation and characterization of NGLY1 patient-derived midbrain organoids.

Frontiers in cell and developmental biology

Abbott J,Tambe M,Pavlinov I,Farkhondeh A,Nguyen HN,Xu M,Pradhan M,York T,Might M,Baumgärtel K,Rodems S,Zheng W

450-10 was used in Cell Culture to provide a relevant NGLY1 disease model to investigate disease mechanisms and evaluate therapeutics for treatments of NGLY1 deficiency.

Tue Mar 07 00:00:00 EST 2023

Multiple genes in a single GWAS risk locus synergistically mediate aberrant synaptic development and function in human neurons.

Cell genomics

Zhang S,Zhang H,Forrest MP,Zhou Y,Sun X,Bagchi VA,Kozlova A,Santos MD,Piguel NH,Dionisio LE,Sanders AR,Pang ZP,He X,Penzes P,Duan J

450-10 was used in Cell Culture to reveal that multiple genes at a single GWAS risk locus mediate a compound effect on neural function, providing a mechanistic link between a non-coding risk variant and disease-related cellular phenotypes.

Wed Sep 13 00:00:00 EDT 2023

Real-time thiol detection in iPSC-derived neuron cultures using SemKur-IM, a novel fluorescent dithio probe.

SLAS discovery : advancing life sciences R & D

Alvarez R,Kurfis J,Hendrickson M,Sem DS

450-10 was used in Cell Culture to demonstrate the utility of SemKur-IM in the detection of thiol levels in live cells in response to chemical exposures, such as from drugs that return the cell to a healthier reduced state.

Mon Nov 20 00:00:00 EST 2023

Circadian clock regulator Bmal1 gates axon regeneration via Tet3 epigenetics in mouse sensory neurons.

Nature communications

Halawani D,Wang Y,Ramakrishnan A,Estill M,He X,Shen L,Friedel RH,Zou H

450-10 was used in Cell Culture to demonstrate that targeting Bmal1 enhances axon regeneration.

Thu Aug 24 00:00:00 EDT 2023

Accelerated differentiation of human induced pluripotent stem cells into regionally specific dorsal and ventral spinal neural progenitor cells for application in spinal cord therapeutics.

Frontiers in neuroscience

Huntemer-Silveira A,Malone D,Frie A,Walsh P,Parr AM

450-10 was used in In vitro experiments to describe here the production of a diverse population of both ventral spinal and dorsal horn progenitor cells.

Sat Feb 10 00:00:00 EST 2024

Glycolysis mediates neuron specific histone acetylation in valproic acid-induced human excitatory neuron differentiation.

Frontiers in molecular neuroscience

Chen A,Wang M,Xu C,Zhao Y,Xian P,Li Y,Zheng W,Yi X,Wu S,Wang Y

450-10 was used in Cell Culture to demonstrate that VPA biased excitatory neuron differentiation by glycolysis-mediated histone acetylation of neuron specific transcription factors.

Tue Apr 25 00:00:00 EDT 2023

Human iPS cell-derived sensory neurons can be infected by SARS-CoV-2.

iScience

Flamier A,Bisht P,Richards A,Tomasello DL,Jaenisch R

450-10 was used in Cell Culture to indicate that sensory neurons can be infected by the original WA1/2020 strain of SARS-CoV-2 as well as the delta and omicron variants, yet infectability differs between the original strain and the variants.

Fri Sep 15 00:00:00 EDT 2023

Three-dimensional co-culture platform of human induced pluripotent stem cell-derived oligodendrocyte lineage cells and neurons for studying myelination.

STAR protocols

von der Bey M,De Cicco S,Zach S,Hengerer B,Ercan-Herbst E

450-10 was used in In vitro experiments to describe steps to differentiate hiPSCs into cortical neurons and oligodendrocyte lineage cells on 3D nanofibers.

Fri Mar 17 00:00:00 EDT 2023

Parkinson's Disease Phenotypes in Patient Neuronal Cultures and Brain Organoids Improved by 2-Hydroxypropyl-β-Cyclodextrin Treatment.

Movement disorders : official journal of the Movement Disorder Society

Jarazo J,Barmpa K,Modamio J,Saraiva C,Sabaté-Soler S,Rosety I,Griesbeck A,Skwirblies F,Zaffaroni G,Smits LM,Su J,Arias-Fuenzalida J,Walter J,Gomez-Giro G,Monzel AS,Qing X,Vitali A,Cruciani G,Boussaad I,Brunelli F,Jäger C,Rakovic A,Li W,Yuan L,Berger E,Arena G,Bolognin S,Schmidt R,Schröder C,Antony PMA,Klein C,Krüger R,Seibler P,Schwamborn JC

450-10 was used in Cell Culture to investigate the effects of biallelic mutations in phosphatase and tensin homolog-induced putative kinase 1 on Parkinson's disease.

Sat Jan 01 00:00:00 EST 2022

Parkinson's Disease Phenotypes in Patient Neuronal Cultures and Brain Organoids Improved by 2-Hydroxypropyl-β-Cyclodextrin Treatment.

Movement disorders : official journal of the Movement Disorder Society

450-10 was used in Cell Culture to investigate the effects of biallelic mutations in phosphatase and tensin homolog-induced putative kinase 1 on Parkinson's disease.

Sat Jan 01 00:00:00 EST 2022

Spinal dI4 Interneuron Differentiation From Human Pluripotent Stem Cells.

Frontiers in molecular neuroscience

Xu J,Huang LJ,Fang Z,Luo HM,Chen YQ,Li YJ,Gong CZ,Chen H

450-10 was used in Cell Culture to investigate spinal interneuron targeting in spinal cord injury treatment.

Fri Apr 29 00:00:00 EDT 2022

Recapitulation of endogenous 4R tau expression and formation of insoluble tau in directly reprogrammed human neurons.

Cell stem cell

Capano LS,Sato C,Ficulle E,Yu A,Horie K,Kwon JS,Burbach KF,Barthélemy NR,Fox SG,Karch CM,Bateman RJ,Houlden H,Morimoto RI,Holtzman DM,Duff KE,Yoo AS

450-10 was used in In vitro experiments to demonstrate the utility of miRNA-induced neuronal reprogramming to recapitulate endogenous tau regulation comparable with the adult brain in health and disease.

Thu Jun 02 00:00:00 EDT 2022

The mitochondrial RNA granule modulates manganese-dependent cell toxicity.

Molecular biology of the cell

Werner E,Gokhale A,Ackert M,Xu C,Wen Z,Roberts AM,Roberts BR,Vrailas-Mortimer A,Crocker A,Faundez V

450-10 was used in Cell Culture to conclude that impairment of the mitochondrial RNA granule function is a protective mechanism for acute manganese toxicity.

Sat Oct 01 00:00:00 EDT 2022

A next-generation iPSC-derived forebrain organoid model of tauopathy with tau fibrils by AAV-mediated gene transfer.

Cell reports methods

Shimada H,Sato Y,Sasaki T,Shimozawa A,Imaizumi K,Shindo T,Miyao S,Kiyama K,Kondo T,Shibata S,Ishii S,Kuromitsu J,Aoyagi H,Ito D,Okano H

450-10 was used in Tissue/organ culture to show that this model can be used as a platform for investigating pathogenetic mechanisms and evaluation of target molecules for drug discovery for tauopathy.

Mon Sep 19 00:00:00 EDT 2022

DYT6 mutated THAP1 is a cell type dependent regulator of the SP1 family.

Brain : a journal of neurology

Cheng F,Zheng W,Barbuti PA,Bonsi P,Liu C,Casadei N,Ponterio G,Meringolo M,Admard J,Dording CM,Yu-Taeger L,Nguyen HP,Grundmann-Hauser K,Ott T,Houlden H,Pisani A,Krüger R,Riess O

450-10 was used in Cell Culture to characterize the function of THAP1 and contributes to the understanding of the pathogenesis of primary dystonia in humans and rats.

Mon Nov 21 00:00:00 EST 2022

Intronic enhancers of the human SNCA gene predominantly regulate its expression in brain in vivo.

Science advances

Cheng F,Zheng W,Liu C,Barbuti PA,Yu-Taeger L,Casadei N,Huebener-Schmid J,Admard J,Boldt K,Junger K,Ueffing M,Houlden H,Sharma M,Kruger R,Grundmann-Hauser K,Ott T,Riess O

450-10 was used in Cell Culture to identify that the DYT6 gene product THAP1 (THAP domain-containing apoptosis-associated protein 1) and its interaction partner CTCF (CCCTC-binding factor) act as transcription regulators of SNCA.

Fri Nov 25 00:00:00 EST 2022

16pdel lipid changes in iPSC-derived neurons and function of FAM57B in lipid metabolism and synaptogenesis.

iScience

Tomasello DL,Kim JL,Khodour Y,McCammon JM,Mitalipova M,Jaenisch R,Futerman AH,Sive H

450-10 was used in Cell Culture to examine the function of the FAM57B gene to further assess the predicted connection with 16p11.2 deletion syndrome.

Fri Jan 21 00:00:00 EST 2022

A transgenic mouse embryonic stem cell line for puromycin selection of V0_V interneurons from heterogenous induced cultures.

Stem cell research & therapy

Pardieck J,Harb M,Sakiyama-Elbert SE

450-10 was used in Cell Culture to generate a transgenic mESC line to enrich V0V INs from induced cultures to allow for a scalable, enriched population for future in vitro and in vivo studies.

Mon Mar 28 00:00:00 EDT 2022

Innate frequency-discrimination hyperacuity in Williams-Beuren syndrome mice.

Cell

Davenport CM,Teubner BJW,Han SB,Patton MH,Eom TY,Garic D,Lansdell BJ,Shirinifard A,Chang TC,Klein J,Pruett-Miller SM,Blundon JA,Zakharenko SS

450-10 was used in Cell Culture to report that the mouse models of Williams-Beuren syndrome have innately enhanced frequency-discrimination acuity and improved frequency coding in the auditory cortex (ACx).

Thu Oct 13 00:00:00 EDT 2022

Spotting-based differentiation of functional dopaminergic progenitors from human pluripotent stem cells.

Nature protocols

Kim J,Jeon J,Song B,Lee N,Ko S,Cha Y,Leblanc P,Seo H,Kim KS

450-10 was used in Cell Culture to present a protocol detailing how to grow hPSCs such as human induced pluripotent and embryonic stem cells in a physically confined area ('spot') and efficiently differentiate them into a highly enriched population of healthy and functional midbrain dopamine progenitors (mDAPs) and midbrain dopamine neurons (mDANs).

Tue Mar 01 00:00:00 EST 2022

PAAN/MIF nuclease inhibition prevents neurodegeneration in Parkinson's disease.

Cell

Park H,Kam TI,Peng H,Chou SC,Mehrabani-Tabari AA,Song JJ,Yin X,Karuppagounder SS,Umanah GK,Rao AVS,Choi Y,Aggarwal A,Chang S,Kim H,Byun J,Liu JO,Dawson TM,Dawson VL

450-10 was used in Cell Culture to show that neurodegeneration induced by pathologic α-synuclein (α-syn) occurs via PAAN/MIF nuclease activity.

Thu May 26 00:00:00 EDT 2022

Prolonged culturing of iPSC-derived brain endothelial-like cells is associated with quiescence, downregulation of glycolysis, and resistance to disruption by an Alzheimer's brain milieu.

Fluids and barriers of the CNS

Williams LM,Fujimoto T,Weaver RR,Logsdon AF,Evitts KM,Young JE,Banks WA,Erickson MA

450-10 was used in Cell Culture to study responses to disease-relevant stimuli in proliferating vs. more quiescent endothelial cell states.

Sat Feb 05 00:00:00 EST 2022

Type-I-interferon signaling drives microglial dysfunction and senescence in human iPSC models of Down syndrome and Alzheimer's disease.

Cell stem cell

Jin M,Xu R,Wang L,Alam MM,Ma Z,Zhu S,Martini AC,Jadali A,Bernabucci M,Xie P,Kwan KY,Pang ZP,Head E,Liu Y,Hart RP,Jiang P

450-10 was used in Cell Culture to provide in vivo evidence that human microglia respond to pathological tau by exhibiting dystrophic phenotypes.

Thu Jul 07 00:00:00 EDT 2022

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics.

Journal of visualized experiments : JoVE

Frankenfield A,Ni J,Hao L

450-10 was used in Cell Culture to demonstrate a detailed protocol for endogenous (knock-in) lysosome proximity labeling proteomic method in human induced pluripotent stem cell (hiPSC)-derived neurons.

Thu Jun 23 00:00:00 EDT 2022

Alzheimer's disease like neuropathology in Down syndrome cortical organoids.

Frontiers in cellular neuroscience

Zhao HH,Haddad GG

450-10 was used in Cell Culture to use induced pluripotent stem cell (iPSC) and iPSC-derived 3D cortical organoids to model Alzheimer's disease in Down syndrome and explore the earliest cellular and molecular changes during DS fetal brain development.

Tue Jan 03 00:00:00 EST 2023

Pharmacological Perturbation of Mechanical Contractility Enables Robust Transdifferentiation of Human Fibroblasts into Neurons.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

He ZQ,Li YH,Feng GH,Yuan XW,Lu ZB,Dai M,Hu YP,Zhang Y,Zhou Q,Li W

450-10 was used in Cell Culture to report a robust method to convert fibroblasts to neurons through targeting the cytoskeleton followed by exposure to lineage-specification surroundings.

Sun May 01 00:00:00 EDT 2022

Foxa2 and Pet1 Direct and Indirect Synergy Drive Serotonergic Neuronal Differentiation.

Frontiers in neuroscience

Aydin B,Sierk M,Moreno-Estelles M,Tejavibulya L,Kumar N,Flames N,Mahony S,Mazzoni EO

450-10 was used in In vitro experiments to highlight the challenges in formulating generalizable rules for describing the behavior of TF combinations that program distinct neuronal subtypes.

Tue Aug 30 00:00:00 EDT 2022

P97/VCP ATPase inhibitors can rescue p97 mutation-linked motor neuron degeneration.

Brain communications

Wang F,Li S,Wang TY,Lopez GA,Antoshechkin I,Chou TF

450-10 was used in Cell Culture to find that two p97 inhibitors, CB-5083 and NMS-873, restore some dysregulated protein levels.

Tue Aug 30 00:00:00 EDT 2022

Evidence that direct inhibition of transcription factor binding is the prevailing mode of gene and repeat repression by DNA methylation.

Nature genetics

Kaluscha S,Domcke S,Wirbelauer C,Stadler MB,Durdu S,Burger L,Schübeler D

450-10 was used in Cell Culture to reveal methylation-sensitive TFs in vivo and argues that direct inhibition, rather than indirect repression by the tested MBD proteins, is the prevailing mechanism of methylation-mediated repression at regulatory regions and repeats.

Thu Dec 01 00:00:00 EST 2022

Human Endogenous Retrovirus K Envelope in Spinal Fluid of Amyotrophic Lateral Sclerosis Is Toxic.

Annals of neurology

Steiner JP,Bachani M,Malik N,DeMarino C,Li W,Sampson K,Lee MH,Kowalak J,Bhaskar M,Doucet-O'Hare T,Garcia-Montojo M,Cowen M,Smith B,Reoma LB,Medina J,Brunel J,Pierquin J,Charvet B,Perron H,Nath A

450-10 was used in Cell Culture to characterize the neurotoxic properties of HML-2 Env.Env neurotoxicity was detected in ALS cerebrospinal fluid and confirmed using recombinant Env protein in a cell-based assay and a mouse model.

Sat Oct 01 00:00:00 EDT 2022

Primary neurons lacking the SNAREs vti1a and vti1b show altered neuronal development.

Neural development

Bollmann C,Schöning S,Kotschnew K,Grosse J,Heitzig N,Fischer von Mollard G

450-10 was used in Sample Preparation to show the importance of vti1a or vti1b for two pathways of neurite elongation.

Tue Nov 22 00:00:00 EST 2022

H3.3-K27M drives neural stem cell-specific gliomagenesis in a human iPSC-derived model.

Cancer cell

Haag D,Mack N,Benites Goncalves da Silva P,Statz B,Clark J,Tanabe K,Sharma T,Jäger N,Jones DTW,Kawauchi D,Wernig M,Pfister SM

Mon Mar 08 00:00:00 EST 2021

A human forebrain organoid model of fragile X syndrome exhibits altered neurogenesis and highlights new treatment strategies.

Nature neuroscience

Kang Y,Zhou Y,Li Y,Han Y,Xu J,Niu W,Li Z,Liu S,Feng H,Huang W,Duan R,Xu T,Raj N,Zhang F,Dou J,Xu C,Wu H,Bassell GJ,Warren ST,Allen EG,Jin P,Wen Z

Fri Oct 01 00:00:00 EDT 2021

Human Stem Cell-Derived Neurons Repair Circuits and Restore Neural Function.

Cell stem cell

Xiong M,Tao Y,Gao Q,Feng B,Yan W,Zhou Y,Kotsonis TA,Yuan T,You Z,Wu Z,Xi J,Haberman A,Graham J,Block J,Zhou W,Chen Y,Zhang SC

Thu Jan 07 00:00:00 EST 2021

Deconstructing Stepwise Fate Conversion of Human Fibroblasts to Neurons by MicroRNAs.

Cell stem cell

Cates K,McCoy MJ,Kwon JS,Liu Y,Abernathy DG,Zhang B,Liu S,Gontarz P,Kim WK,Chen S,Kong W,Ho JN,Burbach KF,Gabel HW,Morris SA,Yoo AS

Thu Jan 07 00:00:00 EST 2021

Deconstructing Stepwise Fate Conversion of Human Fibroblasts to Neurons by MicroRNAs.

Cell stem cell

Cates K,McCoy MJ,Kwon JS,Liu Y,Abernathy DG,Zhang B,Liu S,Gontarz P,Kim WK,Chen S,Kong W,Ho JN,Burbach KF,Gabel HW,Morris SA,Yoo AS

Thu Jan 07 00:00:00 EST 2021

Transformative Network Modeling of Multi-omics Data Reveals Detailed Circuits, Key Regulators, and Potential Therapeutics for Alzheimer's Disease.

Neuron

Wang M,Li A,Sekiya M,Beckmann ND,Quan X,Schrode N,Fernando MB,Yu A,Zhu L,Cao J,Lyu L,Horgusluoglu E,Wang Q,Guo L,Wang YS,Neff R,Song WM,Wang E,Shen Q,Zhou X,Ming C,Ho SM,Vatansever S,Kaniskan HÜ,Jin J,Zhou MM,Ando K,Ho L,Slesinger PA,Yue Z,Zhu J,Katsel P,Gandy S,Ehrlich ME,Fossati V,Noggle S,Cai D,Haroutunian V,Iijima KM,Schadt E,Brennand KJ,Zhang B

450-10 was used in In vitro experiments to provide not only a global landscape but also detailed signaling circuits of complex molecular interactions in key brain regions affected by LOAD, and the resulting network models will serve as a blueprint for developing next-generation therapeutic agents against LOAD.

Wed Jan 20 00:00:00 EST 2021

Stem cell-derived neurons reflect features of protein networks, neuropathology, and cognitive outcome of their aged human donors.

Neuron

Lagomarsino VN,Pearse RV,Liu L,Hsieh YC,Fernandez MA,Vinton EA,Paull D,Felsky D,Tasaki S,Gaiteri C,Vardarajan B,Lee H,Muratore CR,Benoit CR,Chou V,Fancher SB,He A,Merchant JP,Duong DM,Martinez H,Zhou M,Bah F,Vicent MA,Stricker JMS,Xu J,Dammer EB,Levey AI,Chibnik LB,Menon V,Seyfried NT,De Jager PL,Noggle S,Selkoe DJ,Bennett DA,Young-Pearse TL

Wed Nov 03 00:00:00 EDT 2021

Induced pluripotent stem cell-derived motor neurons of CMT type 2 patients reveal progressive mitochondrial dysfunction.

Brain : a journal of neurology

Van Lent J,Verstraelen P,Asselbergh B,Adriaenssens E,Mateiu L,Verbist C,De Winter V,Eggermont K,Van Den Bosch L,De Vos WH,Timmerman V

450-10 was used in Cell Culture to reveal shared cellular phenotypes across different CMT2 subtypes and suggests that targeting such common pathomechanisms could allow the development of a uniform treatment for CMT2.

Sat Sep 04 00:00:00 EDT 2021

Pharmacological reversal of synaptic and network pathology in human MECP2-KO neurons and cortical organoids.

EMBO molecular medicine

Trujillo CA,Adams JW,Negraes PD,Carromeu C,Tejwani L,Acab A,Tsuda B,Thomas CA,Sodhi N,Fichter KM,Romero S,Zanella F,Sejnowski TJ,Ulrich H,Muotri AR

Mon Jan 11 00:00:00 EST 2021

Modeling genetic epileptic encephalopathies using brain organoids.

EMBO molecular medicine

Steinberg DJ,Repudi S,Saleem A,Kustanovich I,Viukov S,Abudiab B,Banne E,Mahajnah M,Hanna JH,Stern S,Carlen PL,Aqeilan RI

Mon Aug 09 00:00:00 EDT 2021

SLC6A20 transporter: a novel regulator of brain glycine homeostasis and NMDAR function.

EMBO molecular medicine

Bae M,Roh JD,Kim Y,Kim SS,Han HM,Yang E,Kang H,Lee S,Kim JY,Kang R,Jung H,Yoo T,Kim H,Kim D,Oh H,Han S,Kim D,Han J,Bae YC,Kim H,Ahn S,Chan AM,Lee D,Kim JW,Kim E

Fri Feb 05 00:00:00 EST 2021

Targeted attenuation of elevated histone marks at SNCA alleviates α-synuclein in Parkinson's disease.

EMBO molecular medicine

Guhathakurta S,Kim J,Adams L,Basu S,Song MK,Adler E,Je G,Fiadeiro MB,Kim YS

Fri Feb 05 00:00:00 EST 2021

Widespread displacement of DNA- and RNA-binding factors underlies toxicity of arginine-rich cell-penetrating peptides.

The EMBO journal

Lafarga V,Sirozh O,Díaz-López I,Galarreta A,Hisaoka M,Zarzuela E,Boskovic J,Jovanovic B,Fernandez-Leiro R,Muñoz J,Stoecklin G,Ventoso I,Fernandez-Capetillo O

450-10 was used in Cell Culture to show that the toxicity of arginine-rich-cell-penetrating peptides is due to displacement of RNA- and DNA- binding factors from chromatin and mRNA.

Thu Jul 01 00:00:00 EDT 2021

Netrin-1 and its receptor DCC modulate survival and death of dopamine neurons and Parkinson's disease features.

The EMBO journal

Jasmin M,Ahn EH,Voutilainen MH,Fombonne J,Guix C,Viljakainen T,Kang SS,Yu LY,Saarma M,Mehlen P,Ye K

Mon Feb 01 00:00:00 EST 2021

Netrin-1 and its receptor DCC modulate survival and death of dopamine neurons and Parkinson's disease features.

The EMBO journal

Jasmin M,Ahn EH,Voutilainen MH,Fombonne J,Guix C,Viljakainen T,Kang SS,Yu LY,Saarma M,Mehlen P,Ye K

Mon Feb 01 00:00:00 EST 2021

Human cell transformation by combined lineage conversion and oncogene expression.

Oncogene

Sahu B,Pihlajamaa P,Zhang K,Palin K,Ahonen S,Cervera A,Ristimäki A,Aaltonen LA,Hautaniemi S,Taipale J

Wed Sep 01 00:00:00 EDT 2021

Convergent genomic and pharmacological evidence of PI3K/GSK3 signaling alterations in neurons from schizophrenia patients.

Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology

Stertz L,Di Re J,Pei G,Fries GR,Mendez E,Li S,Smith-Callahan L,Raventos H,Tipo J,Cherukuru R,Zhao Z,Liu Y,Jia P,Laezza F,Walss-Bass C

Mon Feb 01 00:00:00 EST 2021

Neonatal cortical astrocytes possess intrinsic potential in neuronal conversion in defined media.

Acta pharmacologica Sinica

Zeng P,Hua QH,Gong JY,Shi CJ,Pi XP,Xie X,Zhang R

450-10 was used in Cell Culture to suggest that neonatal astrocytes from certain brain regions possess intrinsic potential to differentiate/transdifferentiate into neurons which may have clinical relevance in the future.

Mon Nov 01 00:00:00 EDT 2021

APOE4 Affects Basal and NMDAR-Mediated Protein Synthesis in Neurons by Perturbing Calcium Homeostasis.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Ramakrishna S,Jhaveri V,Konings SC,Nawalpuri B,Chakraborty S,Holst B,Schmid B,Gouras GK,Freude KK,Muddashetty RS

450-10 was used in Cell Culture to show that the defective protein synthesis mediated by APOE4 is closely linked to the perturbation of calcium homeostasis caused by APOE4 in neurons.

Wed Oct 20 00:00:00 EDT 2021

Allele-Specific Gene Editing Rescues Pathology in a Human Model of Charcot-Marie-Tooth Disease Type 2E.

Frontiers in cell and developmental biology

Feliciano CM,Wu K,Watry HL,Marley CBE,Ramadoss GN,Ghanim HY,Liu AZ,Zholudeva LV,McDevitt TC,Saporta MA,Conklin BR,Judge LM

450-10 was used in Cell Culture to study how allele-specific CRISPR gene editing in a human model of axonal Charcot-Marie-Tooth (CMT) disease rescues pathology caused by a dominant missense mutation in the neurofilament light chain gene (NEFL, CMT type 2E).

Thu Sep 07 00:00:00 EDT 2023

Altered cytoskeletal arrangement in induced pluripotent stem cells (iPSCs) and motor neurons from patients with riboflavin transporter deficiency.

Disease models & mechanisms

Niceforo A,Marioli C,Colasuonno F,Petrini S,Massey K,Tartaglia M,Bertini E,Moreno S,Compagnucci C

450-10 was used in Cell Culture to show that RF administration resulted in partially improved cytoskeletal features in patients' iPSCs and MNs.

Tue Jan 19 00:00:00 EST 2021

Human Induced Pluripotent Stem Cell Derived Sensory Neurons are Sensitive to the Neurotoxic Effects of Paclitaxel.

Clinical and translational science

Xiong C,Chua KC,Stage TB,Priotti J,Kim J,Altman-Merino A,Chan D,Saraf K,Canato Ferracini A,Fattahi F,Kroetz DL

Mon Mar 01 00:00:00 EST 2021

Induction of Ventral Spinal V0 Interneurons from Mouse Embryonic Stem Cells.

Stem cells and development

Pardieck J,Harb M,Sakiyama-Elbert S

450-10 was used in Cell Culture to investigate V0 interneuron isolation to improve understanding of spinal central pattern generators.

Sun Aug 15 00:00:00 EDT 2021

Electroacupuncture Promotes the Survival of the Grafted Human MGE Neural Progenitors in Rats with Cerebral Ischemia by Promoting Angiogenesis and Inhibiting Inflammation.

Neural plasticity

Li J,Chen L,Li D,Lu M,Huang X,Han X,Chen H

Wed Mar 02 00:00:00 EST 2022

Cell-type-specific profiling of human cellular models of fragile X syndrome reveal PI3K-dependent defects in translation and neurogenesis.

Cell reports

Raj N,McEachin ZT,Harousseau W,Zhou Y,Zhang F,Merritt-Garza ME,Taliaferro JM,Kalinowska M,Marro SG,Hales CM,Berry-Kravis E,Wolf-Ochoa MW,Martinez-Cerdeño V,Wernig M,Chen L,Klann E,Warren ST,Jin P,Wen Z,Bassell GJ

Tue Apr 13 00:00:00 EDT 2021

In Vitro Generation of Posterior Motor Neurons from Human Pluripotent Stem Cells.

Current protocols

Wind M,Tsakiridis A

Wed Sep 01 00:00:00 EDT 2021

Efficient gene transfer into zebra finch germline-competent stem cells using an adenoviral vector system.

Scientific reports

Jung KM,Kim YM,Kim JL,Han JY

Tue Jul 20 00:00:00 EDT 2021

Transcriptional networks identify synaptotagmin-like 3 as a regulator of cortical neuronal migration during early neurodevelopment.

Cell reports

Dong X,Yang L,Liu K,Ji X,Tang C,Li W,Ma L,Mei Y,Peng T,Feng B,Wu Z,Tang Q,Gao Y,Yan K,Zhou W,Xiong M

Tue Mar 02 00:00:00 EST 2021

An iPSC model of hereditary sensory neuropathy-1 reveals L-serine-responsive deficits in neuronal ganglioside composition and axoglial interactions.

Cell reports. Medicine

Clark AJ,Kugathasan U,Baskozos G,Priestman DA,Fugger N,Lone MA,Othman A,Chu KH,Blesneac I,Wilson ER,Laurà M,Kalmar B,Greensmith L,Hornemann T,Platt FM,Reilly MM,Bennett DL

Tue Jul 20 00:00:00 EDT 2021

Human motor units in microfluidic devices are impaired by FUS mutations and improved by HDAC6 inhibition.

Stem cell reports

Stoklund Dittlau K,Krasnow EN,Fumagalli L,Vandoorne T,Baatsen P,Kerstens A,Giacomazzi G,Pavie B,Rossaert E,Beckers J,Sampaolesi M,Van Damme P,Van Den Bosch L

Tue Sep 14 00:00:00 EDT 2021

One-step Reprogramming of Human Fibroblasts into Oligodendrocyte-like Cells by SOX10, OLIG2, and NKX6.2.

Stem cell reports

Chanoumidou K,Hernández-Rodríguez B,Windener F,Thomas C,Stehling M,Mozafari S,Albrecht S,Ottoboni L,Antel J,Kim KP,Velychko S,Cui QL,Xu YKT,Martino G,Winkler J,Schöler HR,Baron-Van Evercooren A,Boespflug-Tanguy O,Vaquerizas JM,Ehrlich M,Kuhlmann T

Tue Apr 13 00:00:00 EDT 2021

Umbilical cord blood-derived microglia-like cells to model COVID-19 exposure.

Translational psychiatry

Sheridan SD,Thanos JM,De Guzman RM,McCrea LT,Horng JE,Fu T,Sellgren CM,Perlis RH,Edlow AG

Fri Mar 19 00:00:00 EDT 2021

Glyoxalase I disruption and external carbonyl stress impair mitochondrial function in human induced pluripotent stem cells and derived neurons.

Translational psychiatry

Hara T,Toyoshima M,Hisano Y,Balan S,Iwayama Y,Aono H,Futamura Y,Osada H,Owada Y,Yoshikawa T

Sat May 08 00:00:00 EDT 2021

SOX10 ablation severely impairs the generation of postmigratory neural crest from human pluripotent stem cells.

Cell death & disease

Lai X,Liu J,Zou Z,Wang Y,Wang Y,Liu X,Huang W,Ma Y,Chen Q,Li F,Wu G,Li W,Wang W,Yuan Y,Jiang B

Fri Aug 27 00:00:00 EDT 2021

Improved modeling of human AD with an automated culturing platform for iPSC neurons, astrocytes and microglia.

Nature communications

Bassil R,Shields K,Granger K,Zein I,Ng S,Chih B

Wed Sep 01 00:00:00 EDT 2021

Human spinal GABA neurons alleviate spasticity and improve locomotion in rats with spinal cord injury.

Cell reports

Gong C,Zheng X,Guo F,Wang Y,Zhang S,Chen J,Sun X,Shah SZA,Zheng Y,Li X,Yin Y,Li Q,Huang X,Guo T,Han X,Zhang SC,Wang W,Chen H

Tue Mar 23 00:00:00 EDT 2021

Epigenetic Small Molecules Rescue Nucleocytoplasmic Transport and DNA Damage Phenotypes in C9ORF72 ALS/FTD.

Brain sciences

Ramic M,Andrade NS,Rybin MJ,Esanov R,Wahlestedt C,Benatar M,Zeier Z

450-10 was used in Cell Culture to characterize the nucleocytoplasmic transport and increased DNA damage disturbances and determine the relative contribution of different dipeptide repeat proteins (DPRs) needed to facilitate the development of novel therapeutics for C9ALS/ frontotemporal dementia (FTD).

Sat Nov 20 00:00:00 EST 2021

Generating Enteric Nervous System Progenitors from Human Pluripotent Stem Cells.

Current protocols

Gogolou A,Frith TJR,Tsakiridis A

Tue Jun 01 00:00:00 EDT 2021

Identification of germ cell-specific Mga variant mRNA that promotes meiosis via impediment of a non-canonical PRC1.

Scientific reports

Kitamura Y,Uranishi K,Hirasaki M,Nishimoto M,Suzuki A,Okuda A

Thu May 06 00:00:00 EDT 2021

Neuronal mitochondrial dysfunction in sporadic amyotrophic lateral sclerosis is developmentally regulated.

Scientific reports

Singh T,Jiao Y,Ferrando LM,Yablonska S,Li F,Horoszko EC,Lacomis D,Friedlander RM,Carlisle DL

Thu Sep 23 00:00:00 EDT 2021

Heterogeneity of neurons reprogrammed from spinal cord astrocytes by the proneural factors Ascl1 and Neurogenin2.

Cell reports

Kempf J,Knelles K,Hersbach BA,Petrik D,Riedemann T,Bednarova V,Janjic A,Simon-Ebert T,Enard W,Smialowski P,Götz M,Masserdotti G

Tue Jul 20 00:00:00 EDT 2021

ApoE-Isoform-Dependent SARS-CoV-2 Neurotropism and Cellular Response.

Cell stem cell

Wang C,Zhang M,Garcia G,Tian E,Cui Q,Chen X,Sun G,Wang J,Arumugaswami V,Shi Y

450-10 was used in In vitro experiments to study whether ApoE4 alters COVID-19 susceptibility or severity and the role of direct viral infection in brain cells to suggest that ApoE4 may play a causal role in COVID-19 severity.

Thu Feb 04 00:00:00 EST 2021

Alpha-synuclein dynamics in induced pluripotent stem cell-derived dopaminergic neurons from a Parkinson's disease patient (PARK4) with SNCA triplication.

FEBS open bio

Fukusumi H,Togo K,Sumida M,Nakamori M,Obika S,Baba K,Shofuda T,Ito D,Okano H,Mochizuki H,Kanemura Y

Mon Feb 01 00:00:00 EST 2021

Differentiation of human adult-derived stem cells towards a neural lineage involves a dedifferentiation event prior to differentiation to neural phenotypes.

Scientific reports

Bueno C,Martínez-Morga M,García-Bernal D,Moraleda JM,Martínez S

Tue Jun 08 00:00:00 EDT 2021

16p11.2 deletion is associated with hyperactivation of human iPSC-derived dopaminergic neuron networks and is rescued by RHOA inhibition in vitro.

Nature communications

Sundberg M,Pinson H,Smith RS,Winden KD,Venugopal P,Tai DJC,Gusella JF,Talkowski ME,Walsh CA,Tegmark M,Sahin M

Tue May 18 00:00:00 EDT 2021

Select neurotrophins promote oligodendrocyte progenitor cell process outgrowth in the presence of chondroitin sulfate proteoglycans.

Journal of neuroscience research

Siebert JR,Osterhout DJ

Thu Apr 01 00:00:00 EDT 2021

PINK1 deficiency impairs adult neurogenesis of dopaminergic neurons.

Scientific reports

Brown SJ,Boussaad I,Jarazo J,Fitzgerald JC,Antony P,Keatinge M,Blechman J,Schwamborn JC,Krüger R,Placzek M,Bandmann O

Tue Mar 23 00:00:00 EDT 2021

An integrated multi-omic analysis of iPSC-derived motor neurons from C9ORF72 ALS patients.

iScience

Li J,Lim RG,Kaye JA,Dardov V,Coyne AN,Wu J,Milani P,Cheng A,Thompson TG,Ornelas L,Frank A,Adam M,Banuelos MG,Casale M,Cox V,Escalante-Chong R,Daigle JG,Gomez E,Hayes L,Holewenski R,Lei S,Lenail A,Lima L,Mandefro B,Matlock A,Panther L,Patel-Murray NL,Pham J,Ramamoorthy D,Sachs K,Shelley B,Stocksdale J,Trost H,Wilhelm M,Venkatraman V,Wassie BT,Wyman S,Yang S,Van Eyk JE,Lloyd TE,Finkbeiner S,Fraenkel E,Rothstein JD,Sareen D,Svendsen CN,Thompson LM

Fri Nov 19 00:00:00 EST 2021

A Parkinson's Disease-relevant Mitochondrial and Neuronal Morphology High-throughput Screening Assay in LUHMES Cells.

Bio-protocol

Leah T,Vazquez-Villaseñor I,Ferraiuolo L,Wharton SB,Mortiboys H

450-10 was used in In vitro experiments to provide detailed instructions for data and statistical analysis, including example calculations of Z'-score to assess statistical effect size across independent experiments.

Tue Jan 05 00:00:00 EST 2021

Early Transcriptional Changes in Rabies Virus-Infected Neurons and Their Impact on Neuronal Functions.

Frontiers in microbiology

Kim S,Larrous F,Varet H,Legendre R,Feige L,Dumas G,Matsas R,Kouroupi G,Grailhe R,Bourhy H

450-10 was used in Cell Culture to study neuronal dysfunctions in the context of rabies virus (RABV) using differentiated neurons to characterize the RABV-induced transcriptomic changes at the early time-points of infection.

Sat Jan 01 00:00:00 EST 2022

Tanc2-mediated mTOR inhibition balances mTORC1/2 signaling in the developing mouse brain and human neurons.

Nature communications

Kim SG,Lee S,Kim Y,Park J,Woo D,Kim D,Li Y,Shin W,Kang H,Yook C,Lee M,Kim K,Roh JD,Ryu J,Jung H,Um SM,Yang E,Kim H,Han J,Heo WD,Kim E

Tue May 11 00:00:00 EDT 2021

MeCP2 controls neural stem cell fate specification through miR-199a-mediated inhibition of BMP-Smad signaling.

Cell reports

Nakashima H,Tsujimura K,Irie K,Imamura T,Trujillo CA,Ishizu M,Uesaka M,Pan M,Noguchi H,Okada K,Aoyagi K,Andoh-Noda T,Okano H,Muotri AR,Nakashima K

Tue May 18 00:00:00 EDT 2021

The difficulty to model Huntington's disease in vitro using striatal medium spiny neurons differentiated from human induced pluripotent stem cells.

Scientific reports

Le Cann K,Foerster A,Rösseler C,Erickson A,Hautvast P,Giesselmann S,Pensold D,Kurth I,Rothermel M,Mattis VB,Zimmer-Bensch G,von Hörsten S,Denecke B,Clarner T,Meents J,Lampert A

Thu Mar 25 00:00:00 EDT 2021

Secreted retrovirus-like GAG-domain-containing protein PEG10 is regulated by UBE3A and is involved in Angelman syndrome pathophysiology.

Cell reports. Medicine

Pandya NJ,Wang C,Costa V,Lopatta P,Meier S,Zampeta FI,Punt AM,Mientjes E,Grossen P,Distler T,Tzouros M,Martí Y,Banfai B,Patsch C,Rasmussen S,Hoener M,Berrera M,Kremer T,Dunkley T,Ebeling M,Distel B,Elgersma Y,Jagasia R

Tue Aug 17 00:00:00 EDT 2021

A mitochondrial membrane-bridging machinery mediates signal transduction of intramitochondrial oxidation.

Nature metabolism

Li L,Conradson DM,Bharat V,Kim MJ,Hsieh CH,Minhas PS,Papakyrikos AM,Durairaj AS,Ludlam A,Andreasson KI,Partridge L,Cianfrocco MA,Wang X

Wed Sep 01 00:00:00 EDT 2021

Deficiency in MT5-MMP Supports Branching of Human iPSCs-Derived Neurons and Reduces Expression of GLAST/S100 in iPSCs-Derived Astrocytes.

Cells

Arnst N,Belio-Mairal P,García-González L,Arnaud L,Greetham L,Nivet E,Rivera S,Dityatev A

450-10 was used in Cell Culture to investigate the effects of MT5-MMP deficiency on neurons and glia using human-induced pluripotent stem cells.

Tue Jul 06 00:00:00 EDT 2021

SETX (senataxin), the helicase mutated in AOA2 and ALS4, functions in autophagy regulation.

Autophagy

Richard P,Feng S,Tsai YL,Li W,Rinchetti P,Muhith U,Irizarry-Cole J,Stolz K,Sanz LA,Hartono S,Hoque M,Tadesse S,Seitz H,Lotti F,Hirano M,Chédin F,Tian B,Manley JL

450-10 was used in Cell Culture to study how mutations in the same SETX (senataxin) protein can lead to different phenotypes.

Sun Aug 01 00:00:00 EDT 2021

CHIP-dependent regulation of the actin cytoskeleton is linked to neuronal cell membrane integrity.

iScience

Dias C,Nita E,Faktor J,Tynan AC,Hernychova L,Vojtesek B,Nylandsted J,Hupp TR,Kunath T,Ball KL

Fri Aug 20 00:00:00 EDT 2021

IFITM proteins promote SARS-CoV-2 infection and are targets for virus inhibition in vitro.

Nature communications

Prelli Bozzo C,Nchioua R,Volcic M,Koepke L,Krüger J,Schütz D,Heller S,Stürzel CM,Kmiec D,Conzelmann C,Müller J,Zech F,Braun E,Groß R,Wettstein L,Weil T,Weiß J,Diofano F,Rodríguez Alfonso AA,Wiese S,Sauter D,Münch J,Goffinet C,Catanese A,Schön M,Boeckers TM,Stenger S,Sato K,Just S,Kleger A,Sparrer KMJ,Kirchhoff F

Wed Jul 28 00:00:00 EDT 2021

Robust neuronal differentiation of human iPSC-derived neural progenitor cells cultured on densely-spaced spiky silicon nanowire arrays.

Scientific reports

Harberts J,Siegmund M,Schnelle M,Zhang T,Lei Y,Yu L,Zierold R,Blick RH

Wed Sep 22 00:00:00 EDT 2021

α-Synuclein mutation impairs processing of endomembrane compartments and promotes exocytosis and seeding of α-synuclein pathology.

Cell reports

Stykel MG,Humphries KM,Kamski-Hennekam E,Buchner-Duby B,Porte-Trachsel N,Ryan T,Coackley CL,Bamm VV,Harauz G,Ryan SD

Tue May 11 00:00:00 EDT 2021

Parkinson's disease patient-specific neuronal networks carrying the LRRK2 G2019S mutation unveil early functional alterations that predate neurodegeneration.

NPJ Parkinson's disease

Carola G,Malagarriga D,Calatayud C,Pons-Espinal M,Blasco-Agell L,Richaud-Patin Y,Fernandez-Carasa I,Baruffi V,Beltramone S,Molina E,Dell'Era P,Toledo-Aral JJ,Tolosa E,Muotri AR,Garcia Ojalvo J,Soriano J,Raya A,Consiglio A

Fri Jul 02 00:00:00 EDT 2021

Parkinson's disease patient-specific neuronal networks carrying the LRRK2 G2019S mutation unveil early functional alterations that predate neurodegeneration.

NPJ Parkinson's disease

Fri Jul 02 00:00:00 EDT 2021

Establishment of an Electrophysiological Platform for Modeling ALS with Regionally-Specific Human Pluripotent Stem Cell-Derived Astrocytes and Neurons.

Journal of visualized experiments : JoVE

Taga A,Habela CW,Johns A,Liu S,O'Brien M,Maragakis NJ

450-10 was used in Cell Culture to describe a method to co-culture spinal cord hiPSC-A with hiPSC-MN and record electrophysiological activity using MEA recordings.

Thu Aug 26 00:00:00 EDT 2021

Loss of function mutations in GEMIN5 cause a neurodevelopmental disorder.

Nature communications

Kour S,Rajan DS,Fortuna TR,Anderson EN,Ward C,Lee Y,Lee S,Shin YB,Chae JH,Choi M,Siquier K,Cantagrel V,Amiel J,Stolerman ES,Barnett SS,Cousin MA,Castro D,McDonald K,Kirmse B,Nemeth AH,Rajasundaram D,Innes AM,Lynch D,Frosk P,Collins A,Gibbons M,Yang M,Desguerre I,Boddaert N,Gitiaux C,Rydning SL,Selmer KK,Urreizti R,Garcia-Oguiza A,Osorio AN,Verdura E,Pujol A,McCurry HR,Landers JE,Agnihotri S,Andriescu EC,Moody SB,Phornphutkul C,Sacoto MJG,Begtrup A,Houlden H,Kirschner J,Schorling D,Rudnik-Schöneborn S,Strom TM,Leiz S,Juliette K,Richardson R,Yang Y,Zhang Y,Wang M,Wang J,Wang X,Platzer K,Donkervoort S,Bönnemann CG,Wagner M,Issa MY,Elbendary HM,Stanley V,Maroofian R,Gleeson JG,Zaki MS,Senderek J,Pandey UB

Fri May 07 00:00:00 EDT 2021

Loss of function mutations in GEMIN5 cause a neurodevelopmental disorder.

Nature communications

Fri May 07 00:00:00 EDT 2021

STING regulates peripheral nerve regeneration and colony stimulating factor 1 receptor (CSF1R) processing in microglia.

iScience

Morozzi G,Rothen J,Toussaint G,De Lange K,Westritschnig K,Doelemeyer A,Ueberschlag VP,Kahle P,Lambert C,Obrecht M,Beckmann N,Ritter V,Panesar M,Stauffer D,Garnier I,Mueller M,Guerini D,Keller CG,Knehr J,Roma G,Bidinosti M,Brachat S,Morvan F,Fornaro M

450-10 was used in In vitro experiments to investigate relationship between peripheral nerve injury and inflammation.

Fri Dec 17 00:00:00 EST 2021

An Organoid Biobank of Neuroendocrine Neoplasms Enables Genotype-Phenotype Mapping.

Cell

Kawasaki K,Toshimitsu K,Matano M,Fujita M,Fujii M,Togasaki K,Ebisudani T,Shimokawa M,Takano A,Takahashi S,Ohta Y,Nanki K,Igarashi R,Ishimaru K,Ishida H,Sukawa Y,Sugimoto S,Saito Y,Maejima K,Sasagawa S,Lee H,Kim HG,Ha K,Hamamoto J,Fukunaga K,Maekawa A,Tanabe M,Ishihara S,Hamamoto Y,Yasuda H,Sekine S,Kudo A,Kitagawa Y,Kanai T,Nakagawa H,Sato T

Wed Nov 25 00:00:00 EST 2020

SARS-CoV-2 infects human neural progenitor cells and brain organoids.

Cell research

Zhang BZ,Chu H,Han S,Shuai H,Deng J,Hu YF,Gong HR,Lee AC,Zou Z,Yau T,Wu W,Hung IF,Chan JF,Yuen KY,Huang JD

Thu Oct 01 00:00:00 EDT 2020

Human Pluripotent Stem Cell-Derived Neural Cells and Brain Organoids Reveal SARS-CoV-2 Neurotropism Predominates in Choroid Plexus Epithelium.

Cell stem cell

Jacob F,Pather SR,Huang WK,Zhang F,Wong SZH,Zhou H,Cubitt B,Fan W,Chen CZ,Xu M,Pradhan M,Zhang DY,Zheng W,Bang AG,Song H,Carlos de la Torre J,Ming GL

Thu Dec 03 00:00:00 EST 2020

Suppression of MEHMO Syndrome Mutation in eIF2 by Small Molecule ISRIB.

Molecular cell

Young-Baird SK,Lourenço MB,Elder MK,Klann E,Liebau S,Dever TE

450-10 was used in In vitro experiments to show that a mutation at the C terminus of eIF2γ impairs CDC123 promotion of eIF2 complex formation and decreases the level of eIF2-GTP-Met-tRNAiMet ternary complexes, using patient-derived induced pluripotent stem cells.

Thu Feb 20 00:00:00 EST 2020

Chimeric Peptide Species Contribute to Divergent Dipeptide Repeat Pathology in c9ALS/FTD and SCA36.

Neuron

McEachin ZT,Gendron TF,Raj N,García-Murias M,Banerjee A,Purcell RH,Ward PJ,Todd TW,Merritt-Garza ME,Jansen-West K,Hales CM,García-Sobrino T,Quintáns B,Holler CJ,Taylor G,San Millán B,Teijeira S,Yamashita T,Ohkubo R,Boulis NM,Xu C,Wen Z,Streichenberger N,Fogel BL,Kukar T,Abe K,Dickson DW,Arias M,Glass JD,Jiang J,Tansey MG,Sobrido MJ,Petrucelli L,Rossoll W,Bassell GJ

Wed Jul 22 00:00:00 EDT 2020

A Cluster of Autism-Associated Variants on X-Linked NLGN4X Functionally Resemble NLGN4Y.

Neuron

Nguyen TA,Wu K,Pandey S,Lehr AW,Li Y,Bemben MA,Badger JD,Lauzon JL,Wang T,Zaghloul KA,Thurm A,Jain M,Lu W,Roche KW

Wed Jun 03 00:00:00 EDT 2020

G₄C₂ Repeat RNA Initiates a POM121-Mediated Reduction in Specific Nucleoporins in C9orf72 ALS/FTD.

Neuron

Coyne AN,Zaepfel BL,Hayes L,Fitchman B,Salzberg Y,Luo EC,Bowen K,Trost H,Aigner S,Rigo F,Yeo GW,Harel A,Svendsen CN,Sareen D,Rothstein JD

450-10 was used in Cell Culture to suggest that the expression of expanded C9orf72 ALS/FTD repeat RNA alone affects nuclear POM121 expression in the initiation of a pathological cascade affecting nucleoporin levels within neuronal nuclei and ultimately downstream neuronal survival.

Wed Sep 23 00:00:00 EDT 2020

Disease-specific phenotypes in iPSC-derived neural stem cells with POLG mutations.

EMBO molecular medicine

Liang KX,Kristiansen CK,Mostafavi S,Vatne GH,Zantingh GA,Kianian A,Tzoulis C,Høyland LE,Ziegler M,Perez RM,Furriol J,Zhang Z,Balafkan N,Hong Y,Siller R,Sullivan GJ,Bindoff LA

Wed Oct 07 00:00:00 EDT 2020

Regulation of stem cell function and neuronal differentiation by HERV-K via mTOR pathway.

Proceedings of the National Academy of Sciences of the United States of America

Wang T,Medynets M,Johnson KR,Doucet-O'Hare TT,DiSanza B,Li W,Xu Y,Bagnell A,Tyagi R,Sampson K,Malik N,Steiner J,Hadegan A,Kowalak J,O'Malley J,Maric D,Nath A

Tue Jul 28 00:00:00 EDT 2020

A single-cell Raman-based platform to identify developmental stages of human pluripotent stem cell-derived neurons.

Proceedings of the National Academy of Sciences of the United States of America

Hsu CC,Xu J,Brinkhof B,Wang H,Cui Z,Huang WE,Ye H

Tue Aug 04 00:00:00 EDT 2020

Conserved metabolite regulation of stress granule assembly via AdoMet.

The Journal of cell biology

Begovich K,Vu AQ,Yeo G,Wilhelm JE

Mon Aug 03 00:00:00 EDT 2020

Decellularized skeletal muscles display neurotrophic effects in three-dimensional organotypic cultures.

Stem cells translational medicine

Raffa P,Scattolini V,Gerli MFM,Perin S,Cui M,De Coppi P,Elvassore N,Caccin P,Luni C,Urciuolo A

Thu Oct 01 00:00:00 EDT 2020

3D Bioprinting Pluripotent Stem Cell Derived Neural Tissues Using a Novel Fibrin Bioink Containing Drug Releasing Microspheres.

Frontiers in bioengineering and biotechnology

Sharma R,Smits IPM,De La Vega L,Lee C,Willerth SM

Mon Nov 13 00:00:00 EST 2023

Expression of mutant exon 1 huntingtin fragments in human neural stem cells and neurons causes inclusion formation and mitochondrial dysfunction.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Ghosh R,Wood-Kaczmar A,Dobson L,Smith EJ,Sirinathsinghji EC,Kriston-Vizi J,Hargreaves IP,Heaton R,Herrmann F,Abramov AY,Lam AJ,Heales SJ,Ketteler R,Bates GP,Andre R,Tabrizi SJ

Mon Jun 01 00:00:00 EDT 2020

Generation and optimization of highly pure motor neurons from human induced pluripotent stem cells via lentiviral delivery of transcription factors.

American journal of physiology. Cell physiology

Sepehrimanesh M,Ding B

Thu Oct 01 00:00:00 EDT 2020

Small-Molecule Ligands that Bind the RET Receptor Activate Neuroprotective Signals Independent of but Modulated by Coreceptor GFRα1.

Molecular pharmacology

Jmaeff S,Sidorova Y,Lippiatt H,Barcelona PF,Nedev H,Saragovi LM,Hancock MA,Saarma M,Saragovi HU

450-10 was used in Cell Culture to validate RET receptor as a druggable therapeutic target and provide for potential leads to evaluate in neurodegenerative states.

Wed Jul 01 00:00:00 EDT 2020

Synaptic signalling in a network of dopamine neurons: what prevents proper intercellular crosstalk?

FEBS letters

Chen Y,Kunath T,Simpson J,Homer N,Sylantyev S

Thu Oct 01 00:00:00 EDT 2020

Calpain system is altered in survival motor neuron-reduced cells from in vitro and in vivo spinal muscular atrophy models.

Cell death & disease

de la Fuente S,Sansa A,Hidalgo I,Vivancos N,Romero-Guevara R,Garcera A,Soler RM

Thu Jun 25 00:00:00 EDT 2020

Small-molecule agonists of the RET receptor tyrosine kinase activate biased trophic signals that are influenced by the presence of GFRa1 co-receptors.

The Journal of biological chemistry

Jmaeff S,Sidorova Y,Nedev H,Saarma M,Saragovi HU

Fri May 08 00:00:00 EDT 2020

Single-Cell Transcriptomics of Parkinson's Disease Human In Vitro Models Reveals Dopamine Neuron-Specific Stress Responses.

Cell reports

Fernandes HJR,Patikas N,Foskolou S,Field SF,Park JE,Byrne ML,Bassett AR,Metzakopian E

450-10 was used in Cell Culture to reveal cell type-specific perturbations in human dopamine neurons, which will further our understanding of PD and have implications for cell replacement therapies.

Tue Oct 13 00:00:00 EDT 2020

In Vitro CRISPR/Cas9-Directed Gene Editing to Model LRRK2 G2019S Parkinson's Disease in Common Marmosets.

Scientific reports

Vermilyea SC,Babinski A,Tran N,To S,Guthrie S,Kluss JH,Schmidt JK,Wiepz GJ,Meyer MG,Murphy ME,Cookson MR,Emborg ME,Golos TG

Wed Feb 26 00:00:00 EST 2020

An iPSC-Derived Neuron Model of CLN3 Disease Facilitates Small Molecule Phenotypic Screening.

ACS pharmacology & translational science

Kinarivala N,Morsy A,Patel R,Carmona AV,Sajib MS,Raut S,Mikelis CM,Al-Ahmad A,Trippier PC

450-10 was used in Sample Preparation to report the characterization of the first CLN3 patient-specific induced pluripotent stem cell (iPSC)-derived model of the blood-brain barrier and establish the suitability of an iPSC-derived neuron model of the disease to facilitate compound screening.

Fri Oct 09 00:00:00 EDT 2020

Recapitulation of Neural Crest Specification and EMT via Induction from Neural Plate Border-like Cells.

Stem cell reports

Kobayashi GS,Musso CM,Moreira DP,Pontillo-Guimarães G,Hsia GSP,Caires-Júnior LC,Goulart E,Passos-Bueno MR

Tue Sep 08 00:00:00 EDT 2020

Dipeptide repeat proteins inhibit homology-directed DNA double strand break repair in C9ORF72 ALS/FTD.

Molecular neurodegeneration

Andrade NS,Ramic M,Esanov R,Liu W,Rybin MJ,Gaidosh G,Abdallah A,Del'Olio S,Huff TC,Chee NT,Anatha S,Gendron TF,Wahlestedt C,Zhang Y,Benatar M,Mueller C,Zeier Z

450-10 was used in Cell Culture to study the efficiency of specific repair pathways using DNA double strand break (DSB) repair assays.

Mon Feb 24 00:00:00 EST 2020

The ubiquitin-conjugating enzyme UBE2K determines neurogenic potential through histone H3 in human embryonic stem cells.

Communications biology

Fatima A,Irmak D,Noormohammadi A,Rinschen MM,Das A,Leidecker O,Schindler C,Sánchez-Gaya V,Wagle P,Pokrzywa W,Hoppe T,Rada-Iglesias A,Vilchez D

Mon May 25 00:00:00 EDT 2020

Depletion of the AD Risk Gene SORL1 Selectively Impairs Neuronal Endosomal Traffic Independent of Amyloidogenic APP Processing.

Cell reports

Knupp A,Mishra S,Martinez R,Braggin JE,Szabo M,Kinoshita C,Hailey DW,Small SA,Jayadev S,Young JE

450-10 was used in Cell Culture to validate defects in neuronal endosomal trafficking following the depletion of the SORL1.

Tue Jun 02 00:00:00 EDT 2020

Interferon-γ signaling synergizes with LRRK2 in neurons and microglia derived from human induced pluripotent stem cells.

Nature communications

Panagiotakopoulou V,Ivanyuk D,De Cicco S,Haq W,Arsić A,Yu C,Messelodi D,Oldrati M,Schöndorf DC,Perez MJ,Cassatella RP,Jakobi M,Schneiderhan-Marra N,Gasser T,Nikić-Spiegel I,Deleidi M

Wed Oct 14 00:00:00 EDT 2020

Endothelial, pericyte and tumor cell expression in glioblastoma identifies fibroblast activation protein (FAP) as an excellent target for immunotherapy.

Clinical & translational immunology

Ebert LM,Yu W,Gargett T,Toubia J,Kollis PM,Tea MN,Ebert BW,Bardy C,van den Hurk M,Bonder CS,Manavis J,Ensbey KS,Oksdath Mansilla M,Scheer KG,Perrin SL,Ormsby RJ,Poonnoose S,Koszyca B,Pitson SM,Day BW,Gomez GA,Brown MP

Sun Apr 17 00:00:00 EDT 2022

Proteomics analysis of FUS mutant human motoneurons reveals altered regulation of cytoskeleton and other ALS-linked proteins via 3'UTR binding.

Scientific reports

Garone MG,Alfano V,Salvatori B,Braccia C,Peruzzi G,Colantoni A,Bozzoni I,Armirotti A,Rosa A

Thu Jul 16 00:00:00 EDT 2020

Deficiency of STING Signaling in Embryonic Cerebral Cortex Leads to Neurogenic Abnormalities and Autistic-Like Behaviors.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Zhang D,Liu C,Li H,Jiao J

Tue Dec 01 00:00:00 EST 2020

Identification of candidate genetic variants and altered protein expression in neural stem and mature neural cells support altered microtubule function to be an essential component in bipolar disorder.

Translational psychiatry

Truvé K,Parris TZ,Vizlin-Hodzic D,Salmela S,Berger E,Ågren H,Funa K

Mon Nov 09 00:00:00 EST 2020

Oxidative switch drives mitophagy defects in dopaminergic parkin mutant patient neurons.

Scientific reports

Schwartzentruber A,Boschian C,Lopes FM,Myszczynska MA,New EJ,Beyrath J,Smeitink J,Ferraiuolo L,Mortiboys H

Wed Sep 23 00:00:00 EDT 2020

A fully automated high-throughput workflow for 3D-based chemical screening in human midbrain organoids.

eLife

Renner H,Grabos M,Becker KJ,Kagermeier TE,Wu J,Otto M,Peischard S,Zeuschner D,TsyTsyura Y,Disse P,Klingauf J,Leidel SA,Seebohm G,Schöler HR,Bruder JM

Tue Nov 03 00:00:00 EST 2020

Amyloid precursor protein is a restriction factor that protects against Zika virus infection in mammalian brains.

The Journal of biological chemistry

Lingel A,Lin H,Gavriel Y,Weaver E,Polepole P,Lopez V,Lei Y,Petro TM,Solomon B,Zhang C,Zhang L

Fri Dec 11 00:00:00 EST 2020

Human Pluripotent Stem Cell-Derived Neural Cells and Brain Organoids Reveal SARS-CoV-2 Neurotropism.

bioRxiv : the preprint server for biology

Jacob F,Pather SR,Huang WK,Wong SZH,Zhou H,Zhang F,Cubitt B,Chen CZ,Xu M,Pradhan M,Zhang DY,Zheng W,Bang AG,Song H,de A Torre JC,Ming GL

Tue Jul 28 00:00:00 EDT 2020

Frataxin gene editing rescues Friedreich's ataxia pathology in dorsal root ganglia organoid-derived sensory neurons.

Nature communications

Mazzara PG,Muggeo S,Luoni M,Massimino L,Zaghi M,Valverde PT,Brusco S,Marzi MJ,Palma C,Colasante G,Iannielli A,Paulis M,Cordiglieri C,Giannelli SG,Podini P,Gellera C,Taroni F,Nicassio F,Rasponi M,Broccoli V

Fri Aug 21 00:00:00 EDT 2020

Transplantation of hPSC-derived pericyte-like cells promotes functional recovery in ischemic stroke mice.

Nature communications

Sun J,Huang Y,Gong J,Wang J,Fan Y,Cai J,Wang Y,Qiu Y,Wei Y,Xiong C,Chen J,Wang B,Ma Y,Huang L,Chen X,Zheng S,Huang W,Ke Q,Wang T,Li X,Zhang W,Xiang AP,Li W

Thu Oct 15 00:00:00 EDT 2020

Master Regulators and Cofactors of Human Neuronal Cell Fate Specification Identified by CRISPR Gene Activation Screens.

Cell reports

Black JB,McCutcheon SR,Dube S,Barrera A,Klann TS,Rice GA,Adkar SS,Soderling SH,Reddy TE,Gersbach CA

Tue Dec 01 00:00:00 EST 2020

Accelerated neuronal and synaptic maturation by BrainPhys medium increases Aβ secretion and alters Aβ peptide ratios from iPSC-derived cortical neurons.

Scientific reports

Satir TM,Nazir FH,Vizlin-Hodzic D,Hardselius E,Blennow K,Wray S,Zetterberg H,Agholme L,Bergström P

Fri Jan 17 00:00:00 EST 2020

ASCL1- and DLX2-induced GABAergic neurons from hiPSC-derived NPCs.

Journal of neuroscience methods

Barretto N,Zhang H,Powell SK,Fernando MB,Zhang S,Flaherty EK,Ho SM,Slesinger PA,Duan J,Brennand KJ

450-10 was used in Cell Culture to adapt an ASCL1 and DLX2 overexpression protocol, efficiently generating hiPSC derived NPC-GABAergic neurons, for diease modeling and precision drug screening studies.

Fri Feb 14 00:00:00 EST 2020

Varicella-zoster virus VLT-ORF63 fusion transcript induces broad viral gene expression during reactivation from neuronal latency.

Nature communications

Ouwendijk WJD,Depledge DP,Rajbhandari L,Lenac Rovis T,Jonjic S,Breuer J,Venkatesan A,Verjans GMGM,Sadaoka T

Thu Dec 10 00:00:00 EST 2020

Sequentially induced motor neurons from human fibroblasts facilitate locomotor recovery in a rodent spinal cord injury model.

eLife

Lee H,Lee HY,Lee BE,Gerovska D,Park SY,Zaehres H,Araúzo-Bravo MJ,Kim JI,Ha Y,Schöler HR,Kim JB

450-10 was used in Cell Culture to investigate the use of induced motor neurons to treat spinal cord injury.

Tue Jun 23 00:00:00 EDT 2020

FUS-mediated regulation of acetylcholine receptor transcription at neuromuscular junctions is compromised in amyotrophic lateral sclerosis.

Nature neuroscience

Picchiarelli G,Demestre M,Zuko A,Been M,Higelin J,Dieterlé S,Goy MA,Mallik M,Sellier C,Scekic-Zahirovic J,Zhang L,Rosenbohm A,Sijlmans C,Aly A,Mersmann S,Sanjuan-Ruiz I,Hübers A,Messaddeq N,Wagner M,van Bakel N,Boutillier AL,Ludolph A,Lagier-Tourenne C,Boeckers TM,Dupuis L,Storkebaum E

450-10 was used in Cell Culture to conclude that FUS regulates acetylcholine receptor gene expression in subsynaptic myonuclei, and muscle-intrinsic toxicity of ALS mutant FUS may contribute to dying-back motor neuronopathy.

Fri Nov 01 00:00:00 EDT 2019

OLIG2 Drives Abnormal Neurodevelopmental Phenotypes in Human iPSC-Based Organoid and Chimeric Mouse Models of Down Syndrome.

Cell stem cell

Xu R,Brawner AT,Li S,Liu JJ,Kim H,Xue H,Pang ZP,Kim WY,Hart RP,Liu Y,Jiang P

450-10 was used in Tissue/organ culture to conclude that altered OLIG2 expression may underlie neurodevelopmental abnormalities and cognitive defects in DS patients.

Thu Jun 06 00:00:00 EDT 2019

Neurotrophic factor GDNF regulates intestinal barrier function in inflammatory bowel disease.

The Journal of clinical investigation

Meir M,Burkard N,Ungewiß H,Diefenbacher M,Flemming S,Kannapin F,Germer CT,Schweinlin M,Metzger M,Waschke J,Schlegel N

Mon Jun 17 00:00:00 EDT 2019

Neurotrophic factor GDNF regulates intestinal barrier function in inflammatory bowel disease.

The Journal of clinical investigation

Meir M,Burkard N,Ungewiß H,Diefenbacher M,Flemming S,Kannapin F,Germer CT,Schweinlin M,Metzger M,Waschke J,Schlegel N

Mon Jun 17 00:00:00 EDT 2019

Defining the Functional Role of Na_V1.7 in Human Nociception.

Neuron

McDermott LA,Weir GA,Themistocleous AC,Segerdahl AR,Blesneac I,Baskozos G,Clark AJ,Millar V,Peck LJ,Ebner D,Tracey I,Serra J,Bennett DL

Wed Mar 06 00:00:00 EST 2019

Small-Molecule Modulation of TDP-43 Recruitment to Stress Granules Prevents Persistent TDP-43 Accumulation in ALS/FTD.

Neuron

Fang MY,Markmiller S,Vu AQ,Javaherian A,Dowdle WE,Jolivet P,Bushway PJ,Castello NA,Baral A,Chan MY,Linsley JW,Linsley D,Mercola M,Finkbeiner S,Lecuyer E,Lewcock JW,Yeo GW

Wed Sep 04 00:00:00 EDT 2019

RNA Binding Antagonizes Neurotoxic Phase Transitions of TDP-43.

Neuron

Mann JR,Gleixner AM,Mauna JC,Gomes E,DeChellis-Marks MR,Needham PG,Copley KE,Hurtle B,Portz B,Pyles NJ,Guo L,Calder CB,Wills ZP,Pandey UB,Kofler JK,Brodsky JL,Thathiah A,Shorter J,Donnelly CJ

Wed Apr 17 00:00:00 EDT 2019

Pioneer Factor NeuroD1 Rearranges Transcriptional and Epigenetic Profiles to Execute Microglia-Neuron Conversion.

Neuron

Matsuda T,Irie T,Katsurabayashi S,Hayashi Y,Nagai T,Hamazaki N,Adefuin AMD,Miura F,Ito T,Kimura H,Shirahige K,Takeda T,Iwasaki K,Imamura T,Nakashima K

Wed Feb 06 00:00:00 EST 2019

Quantitative proteomics identifies proteins that resist translational repression and become dysregulated in ALS-FUS.

Human molecular genetics

Baron DM,Matheny T,Lin YC,Leszyk JD,Kenna K,Gall KV,Santos DP,Tischbein M,Funes S,Hayward LJ,Kiskinis E,Landers JE,Parker R,Shaffer SA,Bosco DA

450-10 was used in Cell Culture to suggest that Golgi to ER retrograde transport may be important under conditions of stress and is perturbed upon the expression of disease-linked proteins such as FUS.

Mon Jul 01 00:00:00 EDT 2019

Engineering synucleinopathy-resistant human dopaminergic neurons by CRISPR-mediated deletion of the SNCA gene.

The European journal of neuroscience

Chen Y,Dolt KS,Kriek M,Baker T,Downey P,Drummond NJ,Canham MA,Natalwala A,Rosser S,Kunath T

Fri Feb 01 00:00:00 EST 2019

The testicular soma of Tsc22d3 knockout mice supports spermatogenesis and germline transmission from spermatogonial stem cell lines upon transplantation.

Genesis (New York, N.Y. : 2000)

Zhou H,Zeng Z,Koentgen F,Khan M,Mombaerts P

Sat Jun 01 00:00:00 EDT 2019

Axon-seq for in Depth Analysis of the RNA Content of Neuronal Processes.

Bio-protocol

Nijssen J,Aguila J,Hedlund E

450-10 was used in Cell Culture to evaluate Axon-seq to study both somas, dendrites and axons in isolation.

Sat Jul 20 00:00:00 EDT 2019

Suppression of glioblastoma by a drug cocktail reprogramming tumor cells into neuronal like cells.

Scientific reports

Gao L,Huang S,Zhang H,Hua W,Xin S,Cheng L,Guan W,Yu Y,Mao Y,Pei G

Tue Mar 05 00:00:00 EST 2019

Felodipine induces autophagy in mouse brains with pharmacokinetics amenable to repurposing.

Nature communications

Siddiqi FH,Menzies FM,Lopez A,Stamatakou E,Karabiyik C,Ureshino R,Ricketts T,Jimenez-Sanchez M,Esteban MA,Lai L,Tortorella MD,Luo Z,Liu H,Metzakopian E,Fernandes HJR,Bassett A,Karran E,Miller BL,Fleming A,Rubinsztein DC

Thu Apr 18 00:00:00 EDT 2019

Brain and behavior

Pu J,Dai S,Gao T,Hu J,Fang Y,Zheng R,Jin C,Zhang B

Sun Dec 01 00:00:00 EST 2019

Differentiation but not ALS mutations in FUS rewires motor neuron metabolism.

Nature communications

Vandoorne T,Veys K,Guo W,Sicart A,Vints K,Swijsen A,Moisse M,Eelen G,Gounko NV,Fumagalli L,Fazal R,Germeys C,Quaegebeur A,Fendt SM,Carmeliet P,Verfaillie C,Van Damme P,Ghesquière B,De Bock K,Van Den Bosch L

Thu Sep 12 00:00:00 EDT 2019

3D Cultures of Parkinson's Disease-Specific Dopaminergic Neurons for High Content Phenotyping and Drug Testing.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Bolognin S,Fossépré M,Qing X,Jarazo J,Ščančar J,Moreno EL,Nickels SL,Wasner K,Ouzren N,Walter J,Grünewald A,Glaab E,Salamanca L,Fleming RMT,Antony PMA,Schwamborn JC

Wed Jan 09 00:00:00 EST 2019

Efficient Generation of Trunk Neural Crest and Sympathetic Neurons from Human Pluripotent Stem Cells Via a Neuromesodermal Axial Progenitor Intermediate.

Current protocols in stem cell biology

Frith TJR,Tsakiridis A

450-10 was used in Cell Culture to describe a detailed protocol for the efficient in vitro generation of trunk neural crest (NC) cells and their derivatives from human pluripotent stem cells (hPSCs).

Sat Jun 01 00:00:00 EDT 2019

Proneural factors Ascl1 and Neurog2 contribute to neuronal subtype identities by establishing distinct chromatin landscapes.

Nature neuroscience

Aydin B,Kakumanu A,Rossillo M,Moreno-Estellés M,Garipler G,Ringstad N,Flames N,Mahony S,Mazzoni EO

450-10 was used in Cell Culture to find that two main vertebrate proneural factors, Ascl1 and neurogenin 2 (Neurog2), induce different neuronal fates by binding to largely different sets of genomic sites, using direct neuronal programming of embryonic stem cells.

Sat Jun 01 00:00:00 EDT 2019

Stem cell-derived cranial and spinal motor neurons reveal proteostatic differences between ALS resistant and sensitive motor neurons.

eLife

An D,Fujiki R,Iannitelli DE,Smerdon JW,Maity S,Rose MF,Gelber A,Wanaselja EK,Yagudayeva I,Lee JY,Vogel C,Wichterle H,Engle EC,Mazzoni EO

450-10 was used in Cell Culture to show an ESC-based platform has identified a superior capacity to maintain a healthy proteome as a possible mechanism to resist ALS-induced neurodegeneration.

Mon Jun 03 00:00:00 EDT 2019

TGF-β-SNAIL axis induces Müller glial-mesenchymal transition in the pathogenesis of idiopathic epiretinal membrane.

Scientific reports

Kanda A,Noda K,Hirose I,Ishida S

Thu Jan 24 00:00:00 EST 2019

A Novel Toolkit for Characterizing the Mechanical and Electrical Properties of Engineered Neural Tissues.

Biosensors

Robinson M,Valente KP,Willerth SM

450-10 was used in Cell Culture to design and validate a set of robust and non-toxic protocols for directly evaluating the properties of engineered neural tissue.

Mon Apr 01 00:00:00 EDT 2019

Automated microfluidic cell culture of stem cell derived dopaminergic neurons.

Scientific reports

Kane KIW,Moreno EL,Hachi S,Walter M,Jarazo J,Oliveira MAP,Hankemeier T,Vulto P,Schwamborn JC,Thoma M,Fleming RMT

Mon Feb 11 00:00:00 EST 2019

Gfra1 Underexpression Causes Hirschsprung's Disease and Associated Enterocolitis in Mice.

Cellular and molecular gastroenterology and hepatology

Porokuokka LL,Virtanen HT,Lindén J,Sidorova Y,Danilova T,Lindahl M,Saarma M,Andressoo JO

450-10 was used in In vitro experiments to suggest that goblet cells may be a potential target for preventative treatment and that reduced expression of GFRa1 may contribute to HSCR susceptibility.

Tue Jun 04 00:00:00 EDT 2019

Sex-specific transcriptional and proteomic signatures in schizophrenia.

Nature communications

Tiihonen J,Koskuvi M,Storvik M,Hyötyläinen I,Gao Y,Puttonen KA,Giniatullina R,Poguzhelskaya E,Ojansuu I,Vaurio O,Cannon TD,Lönnqvist J,Therman S,Suvisaari J,Kaprio J,Cheng L,Hill AF,Lähteenvuo M,Tohka J,Giniatullin R,Lehtonen Š,Koistinaho J

Mon Sep 02 00:00:00 EDT 2019

Gene expression analysis reveals early dysregulation of disease pathways and links Chmp7 to pathogenesis of spinal and bulbar muscular atrophy.

Scientific reports

Malik B,Devine H,Patani R,La Spada AR,Hanna MG,Greensmith L

Tue Mar 05 00:00:00 EST 2019

CRISPR/Cas9-mediated generation of a tyrosine hydroxylase reporter iPSC line for live imaging and isolation of dopaminergic neurons.

Scientific reports

Calatayud C,Carola G,Fernández-Carasa I,Valtorta M,Jiménez-Delgado S,Díaz M,Soriano-Fradera J,Cappelletti G,García-Sancho J,Raya Á,Consiglio A

450-10 was used in Cell Culture to investigate induced pluripotent stem cells use in Parkinson’s.

Thu May 02 00:00:00 EDT 2019

CRISPR/Cas9-mediated generation of a tyrosine hydroxylase reporter iPSC line for live imaging and isolation of dopaminergic neurons.

Scientific reports

Calatayud C,Carola G,Fernández-Carasa I,Valtorta M,Jiménez-Delgado S,Díaz M,Soriano-Fradera J,Cappelletti G,García-Sancho J,Raya Á,Consiglio A

450-10 was used in Cell Culture to investigate induced pluripotent stem cells use in Parkinson’s.

Thu May 02 00:00:00 EDT 2019

Precisely controlling endogenous protein dosage in hPSCs and derivatives to model FOXG1 syndrome.

Nature communications

Zhu W,Zhang B,Li M,Mo F,Mi T,Wu Y,Teng Z,Zhou Q,Li W,Hu B

Mon Feb 25 00:00:00 EST 2019

Leaky Optoelectrical Fiber for Optogenetic Stimulation and Electrochemical Detection of Dopamine Exocytosis from Human Dopaminergic Neurons.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Vasudevan S,Kajtez J,Bunea AI,Gonzalez-Ramos A,Ramos-Moreno T,Heiskanen A,Kokaia M,Larsen NB,Martínez-Serrano A,Keller SS,Emnéus J

Sun Dec 01 00:00:00 EST 2019

Modeling Parkinson's disease in midbrain-like organoids.

NPJ Parkinson's disease

Smits LM,Reinhardt L,Reinhardt P,Glatza M,Monzel AS,Stanslowsky N,Rosato-Siri MD,Zanon A,Antony PM,Bellmann J,Nicklas SM,Hemmer K,Qing X,Berger E,Kalmbach N,Ehrlich M,Bolognin S,Hicks AA,Wegner F,Sterneckert JL,Schwamborn JC

Wed Sep 30 00:00:00 EDT 2020

Preparation and Co-Culture of iPSC-Derived Dopaminergic Neurons and Astrocytes.

Current protocols in cell biology

de Rus Jacquet A

Sun Dec 01 00:00:00 EST 2019

A Simple Procedure for Creating Scalable Phenotypic Screening Assays in Human Neurons.

Scientific reports

Sridharan B,Hubbs C,Llamosas N,Kilinc M,Singhera FU,Willems E,Piper DR,Scampavia L,Rumbaugh G,Spicer TP

Fri Jun 21 00:00:00 EDT 2019

Required growth facilitators propel axon regeneration across complete spinal cord injury.

Nature

Anderson MA,O'Shea TM,Burda JE,Ao Y,Barlatey SL,Bernstein AM,Kim JH,James ND,Rogers A,Kato B,Wollenberg AL,Kawaguchi R,Coppola G,Wang C,Deming TJ,He Z,Courtine G,Sofroniew MV

Sat Sep 01 00:00:00 EDT 2018

Required growth facilitators propel axon regeneration across complete spinal cord injury.

Nature

Anderson MA,O'Shea TM,Burda JE,Ao Y,Barlatey SL,Bernstein AM,Kim JH,James ND,Rogers A,Kato B,Wollenberg AL,Kawaguchi R,Coppola G,Wang C,Deming TJ,He Z,Courtine G,Sofroniew MV

Sat Sep 01 00:00:00 EDT 2018

SIRT6 deficiency results in developmental retardation in cynomolgus monkeys.

Nature

Zhang W,Wan H,Feng G,Qu J,Wang J,Jing Y,Ren R,Liu Z,Zhang L,Chen Z,Wang S,Zhao Y,Wang Z,Yuan Y,Zhou Q,Li W,Liu GH,Hu B

Wed Aug 01 00:00:00 EDT 2018

SIRT6 deficiency results in developmental retardation in cynomolgus monkeys.

Nature

Zhang W,Wan H,Feng G,Qu J,Wang J,Jing Y,Ren R,Liu Z,Zhang L,Chen Z,Wang S,Zhao Y,Wang Z,Yuan Y,Zhou Q,Li W,Liu GH,Hu B

Wed Aug 01 00:00:00 EDT 2018

3D Printed Stem-Cell Derived Neural Progenitors Generate Spinal Cord Scaffolds.

Advanced functional materials

Joung D,Truong V,Neitzke CC,Guo SZ,Walsh PJ,Monat JR,Meng F,Park SH,Dutton JR,Parr AM,McAlpine MC

450-10 was used in Cell Culture to introduce a living platform to model spinal cord cytoarchitecture which could ultimately be used as a therapeutic for chronic spinal cord injury to regenerate axons across the lesion site.

Wed Sep 26 00:00:00 EDT 2018

The COPII cargo adapter SEC24C is essential for neuronal homeostasis.

The Journal of clinical investigation

Wang B,Joo JH,Mount R,Teubner BJW,Krenzer A,Ward AL,Ichhaporia VP,Adams EJ,Khoriaty R,Peters ST,Pruett-Miller SM,Zakharenko SS,Ginsburg D,Kundu M

Wed Aug 01 00:00:00 EDT 2018

Tau Kinetics in Neurons and the Human Central Nervous System.

Neuron

Sato C,Barthélemy NR,Mawuenyega KG,Patterson BW,Gordon BA,Jockel-Balsarotti J,Sullivan M,Crisp MJ,Kasten T,Kirmess KM,Kanaan NM,Yarasheski KE,Baker-Nigh A,Benzinger TLS,Miller TM,Karch CM,Bateman RJ

Wed Mar 21 00:00:00 EDT 2018

Therapeutically targeting tumor microenvironment-mediated drug resistance in estrogen receptor-positive breast cancer.

The Journal of experimental medicine

Shee K,Yang W,Hinds JW,Hampsch RA,Varn FS,Traphagen NA,Patel K,Cheng C,Jenkins NP,Kettenbach AN,Demidenko E,Owens P,Faber AC,Golub TR,Straussman R,Miller TW

Mon Mar 05 00:00:00 EST 2018

Generation of human brain region-specific organoids using a miniaturized spinning bioreactor.

Nature protocols

Qian X,Jacob F,Song MM,Nguyen HN,Song H,Ming GL

Thu Mar 01 00:00:00 EST 2018

Human iPSC-derived trigeminal neurons lack constitutive TLR3-dependent immunity that protects cortical neurons from HSV-1 infection.

Proceedings of the National Academy of Sciences of the United States of America

Zimmer B,Ewaleifoh O,Harschnitz O,Lee YS,Peneau C,McAlpine JL,Liu B,Tchieu J,Steinbeck JA,Lafaille F,Volpi S,Notarangelo LD,Casanova JL,Zhang SY,Smith GA,Studer L

Tue Sep 11 00:00:00 EDT 2018

Wnt signal activation induces midbrain specification through direct binding of the beta-catenin/TCF4 complex to the EN1 promoter in human pluripotent stem cells.

Experimental & molecular medicine

Kim JY,Lee JS,Hwang HS,Lee DR,Park CY,Jung SJ,You YR,Kim DS,Kim DW

Fri Apr 13 00:00:00 EDT 2018

GDNF, Neurturin, and Artemin Activate and Sensitize Bone Afferent Neurons and Contribute to Inflammatory Bone Pain.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Nencini S,Ringuet M,Kim DH,Greenhill C,Ivanusic JJ

450-10 was used in In vivo experiments to explore roles for GDNF, neurturin, and artemin signaling in bone pain using male Sprague Dawley rats.

Wed May 23 00:00:00 EDT 2018

Expression Pattern of the Aspartyl-tRNA Synthetase DARS in the Human Brain.

Frontiers in molecular neuroscience

Fröhlich D,Suchowerska AK,Voss C,He R,Wolvetang E,von Jonquieres G,Simons C,Fath T,Housley GD,Klugmann M

450-10 was used in Cell Culture to analyse endogenous DARS expression on the mRNA and protein level in different regions and cell types of human post mortem brain tissue.

Thu Oct 01 00:00:00 EDT 2020

Patch-Seq Protocol to Analyze the Electrophysiology, Morphology and Transcriptome of Whole Single Neurons Derived From Human Pluripotent Stem Cells.

Frontiers in molecular neuroscience

van den Hurk M,Erwin JA,Yeo GW,Gage FH,Bardy C

450-10 was used in In vitro experiments to combine scRNA-seq with patch-clamping electrophysiological recording and morphological analysis of single human neurons in vitro which could be used on neurons in vitro to profile cell types and states in depth to unravel the human molecular basis of neuronal diversity and investigate the cellular mechanisms underlying brain disorders.

Wed Sep 30 00:00:00 EDT 2020

CXCR4/SDF1 signalling promotes sensory neuron clustering in vitro.

Biology open

Terheyden-Keighley D,Zhang X,Brand-Saberi B,Theiss C

450-10 was used in In vitro experiments to investigate mechanisms of dorsal root ganglia formation in development.

Wed Sep 19 00:00:00 EDT 2018

Human Clinical-Grade Parthenogenetic ESC-Derived Dopaminergic Neurons Recover Locomotive Defects of Nonhuman Primate Models of Parkinson's Disease.

Stem cell reports

Wang YK,Zhu WW,Wu MH,Wu YH,Liu ZX,Liang LM,Sheng C,Hao J,Wang L,Li W,Zhou Q,Hu BY

Tue Jul 10 00:00:00 EDT 2018

Macrophage Cytokines Enhance Cell Proliferation of Normal Prostate Epithelial Cells through Activation of ERK and Akt.

Scientific reports

Dang T,Liou GY

Wed May 16 00:00:00 EDT 2018

Convergence of independent DISC1 mutations on impaired neurite growth via decreased UNC5D expression.

Translational psychiatry

Srikanth P,Lagomarsino VN,Pearse RV,Liao M,Ghosh S,Nehme R,Seyfried N,Eggan K,Young-Pearse TL

Thu Nov 08 00:00:00 EST 2018

Testicular Architecture Is Critical for Mediation of Retinoic Acid Responsiveness by Undifferentiated Spermatogonial Subtypes in the Mouse.

Stem cell reports

Lord T,Oatley MJ,Oatley JM

Tue Feb 13 00:00:00 EST 2018

Antagonism between the transcription factors NANOG and OTX2 specifies rostral or caudal cell fate during neural patterning transition.

The Journal of biological chemistry

Su Z,Zhang Y,Liao B,Zhong X,Chen X,Wang H,Guo Y,Shan Y,Wang L,Pan G

Fri Mar 23 00:00:00 EDT 2018

Preferential amplification of a human mitochondrial DNA deletion in vitro and in vivo.

Scientific reports

Russell OM,Fruh I,Rai PK,Marcellin D,Doll T,Reeve A,Germain M,Bastien J,Rygiel KA,Cerino R,Sailer AW,Lako M,Taylor RW,Mueller M,Lightowlers RN,Turnbull DM,Helliwell SB

Mon Jan 29 00:00:00 EST 2018

Integrative system biology analyses of CRISPR-edited iPSC-derived neurons and human brains reveal deficiencies of presynaptic signaling in FTLD and PSP.

Translational psychiatry

Jiang S,Wen N,Li Z,Dube U,Del Aguila J,Budde J,Martinez R,Hsu S,Fernandez MV,Cairns NJ,Harari O,Cruchaga C,Karch CM

Thu Dec 13 00:00:00 EST 2018

Insights into the ubiquitin-proteasome system of human embryonic stem cells.

Scientific reports

Saez I,Koyuncu S,Gutierrez-Garcia R,Dieterich C,Vilchez D

Tue Mar 06 00:00:00 EST 2018

Melatonin attenuates detrimental effects of diabetes on the niche of mouse spermatogonial stem cells by maintaining Leydig cells.

Cell death & disease

Du Z,Xu S,Hu S,Yang H,Zhou Z,Sidhu K,Miao Y,Liu Z,Shen W,Reiter RJ,Hua J,Peng S

Thu Sep 20 00:00:00 EDT 2018

Embryonic Stem Cells Derived Kidney Organoids as Faithful Models to Target Programmed Nephrogenesis.

Scientific reports

Tan Z,Shan J,Rak-Raszewska A,Vainio SJ

Fri Nov 09 00:00:00 EST 2018

Differential antiviral immunity to Japanese encephalitis virus in developing cortical organoids.

Cell death & disease

Zhang B,He Y,Xu Y,Mo F,Mi T,Shen QS,Li C,Li Y,Liu J,Wu Y,Chen G,Zhu W,Qin C,Hu B,Zhou G

Mon Jun 18 00:00:00 EDT 2018

Hypertonic Stress Causes Cytoplasmic Translocation of Neuronal, but Not Astrocytic, FUS due to Impaired Transportin Function.

Cell reports

Hock EM,Maniecka Z,Hruska-Plochan M,Reber S,Laferrière F,Sahadevan M K S,Ederle H,Gittings L,Pelkmans L,Dupuis L,Lashley T,Ruepp MD,Dormann D,Polymenidou M

450-10 was used in In vitro experiments to study the stress-induced nucleocytoplasmic shuttling of endogenous FUS in an ex vivo mouse central nervous system model and human neural networks.

Tue Jul 24 00:00:00 EDT 2018

Hypertonic Stress Causes Cytoplasmic Translocation of Neuronal, but Not Astrocytic, FUS due to Impaired Transportin Function.

Cell reports

Hock EM,Maniecka Z,Hruska-Plochan M,Reber S,Laferrière F,Sahadevan M K S,Ederle H,Gittings L,Pelkmans L,Dupuis L,Lashley T,Ruepp MD,Dormann D,Polymenidou M

450-10 was used in In vitro experiments to study the stress-induced nucleocytoplasmic shuttling of endogenous FUS in an ex vivo mouse central nervous system model and human neural networks.

Tue Jul 24 00:00:00 EDT 2018

Hypertonic Stress Causes Cytoplasmic Translocation of Neuronal, but Not Astrocytic, FUS due to Impaired Transportin Function.

Cell reports

Hock EM,Maniecka Z,Hruska-Plochan M,Reber S,Laferrière F,Sahadevan M K S,Ederle H,Gittings L,Pelkmans L,Dupuis L,Lashley T,Ruepp MD,Dormann D,Polymenidou M

450-10 was used in In vitro experiments to study the stress-induced nucleocytoplasmic shuttling of endogenous FUS in an ex vivo mouse central nervous system model and human neural networks.

Tue Jul 24 00:00:00 EDT 2018

PTEN expression by an oncolytic herpesvirus directs T-cell mediated tumor clearance.

Nature communications

Russell L,Swanner J,Jaime-Ramirez AC,Wang Y,Sprague A,Banasavadi-Siddegowda Y,Yoo JY,Sizemore GM,Kladney R,Zhang J,Lehman NL,Ostrowski MC,Hong B,Caligiuri M,Yu J,Kaur B

Tue Nov 27 00:00:00 EST 2018

Cell death & disease

Birger A,Ottolenghi M,Perez L,Reubinoff B,Behar O

Thu Feb 15 00:00:00 EST 2018

Human Cortex Spheroid with a Functional Blood Brain Barrier for High-Throughput Neurotoxicity Screening and Disease Modeling.

Scientific reports

Nzou G,Wicks RT,Wicks EE,Seale SA,Sane CH,Chen A,Murphy SV,Jackson JD,Atala AJ

Wed May 09 00:00:00 EDT 2018

A bi-stable feedback loop between GDNF, EGR1, and ERα contribute to endocrine resistant breast cancer.

PloS one

Horibata S,Rice EJ,Zheng H,Mukai C,Chu T,Marks BA,Coonrod SA,Danko CG

450-10 was used in Cell Culture to discover interactions between GDNF, EGR1, ERα, MAPK, SRF, AP-1 in cells.

Mon Jul 16 00:00:00 EDT 2018

Chemical conversion of human lung fibroblasts into neuronal cells.

International journal of molecular medicine

Wan XY,Xu LY,Li B,Sun QH,Ji QL,Huang DD,Zhao L,Xiao YT

450-10 was used in Cell Culture to investigate whether a cocktail of small molecules can convert human lung fibroblast cells into neurons.

Thu Mar 01 00:00:00 EST 2018

Full-length amyloid precursor protein regulates lipoprotein metabolism and amyloid-β clearance in human astrocytes.

The Journal of biological chemistry

Fong LK,Yang MM,Dos Santos Chaves R,Reyna SM,Langness VF,Woodruff G,Roberts EA,Young JE,Goldstein LSB

Fri Jul 20 00:00:00 EDT 2018

A Chemical Recipe for Generation of Clinical-Grade Striatal Neurons from hESCs.

Stem cell reports

Wu M,Zhang D,Bi C,Mi T,Zhu W,Xia L,Teng Z,Hu B,Wu Y

Tue Sep 11 00:00:00 EDT 2018

High-efficiency RNA-based reprogramming of human primary fibroblasts.

Nature communications

Kogut I,McCarthy SM,Pavlova M,Astling DP,Chen X,Jakimenko A,Jones KL,Getahun A,Cambier JC,Pasmooij AMG,Jonkman MF,Roop DR,Bilousova G

Wed Feb 21 00:00:00 EST 2018

Lysosomal and network alterations in human mucopolysaccharidosis type VII iPSC-derived neurons.

Scientific reports

Bayó-Puxan N,Terrasso AP,Creyssels S,Simão D,Begon-Pescia C,Lavigne M,Salinas S,Bernex F,Bosch A,Kalatzis V,Levade T,Cuervo AM,Lory P,Consiglio A,Brito C,Kremer EJ

Fri Nov 09 00:00:00 EST 2018

Aberrant Calcium Signaling in Astrocytes Inhibits Neuronal Excitability in a Human Down Syndrome Stem Cell Model.

Cell reports

Mizuno GO,Wang Y,Shi G,Wang Y,Sun J,Papadopoulos S,Broussard GJ,Unger EK,Deng W,Weick J,Bhattacharyya A,Chen CY,Yu G,Looger LL,Tian L

450-10 was used in Cell Culture to investigate HSA21 mutations on Down syndrome.

Tue Jul 10 00:00:00 EDT 2018

Mutant FUS causes DNA ligation defects to inhibit oxidative damage repair in Amyotrophic Lateral Sclerosis.

Nature communications

Wang H,Guo W,Mitra J,Hegde PM,Vandoorne T,Eckelmann BJ,Mitra S,Tomkinson AE,Van Den Bosch L,Hegde ML

Tue Sep 11 00:00:00 EDT 2018

hPSC-Derived Striatal Cells Generated Using a Scalable 3D Hydrogel Promote Recovery in a Huntington Disease Mouse Model.

Stem cell reports

Adil MM,Gaj T,Rao AT,Kulkarni RU,Fuentes CM,Ramadoss GN,Ekman FK,Miller EW,Schaffer DV

Tue May 08 00:00:00 EDT 2018

ER-positive breast cancer cells are poised for RET-mediated endocrine resistance.

PloS one

Horibata S,Rice EJ,Mukai C,Marks BA,Sams K,Zheng H,Anguish LJ,Coonrod SA,Danko CG

450-10 was used in In vitro experiments to investigate RET tyrosine kinase signalling in ER+ breast cancer.

Tue Jul 10 00:00:00 EDT 2018

GDNF enhances human blood-nerve barrier function in vitro via MAPK signaling pathways.

Tissue barriers

Dong C,Ubogu EE

450-10 was used in Protein Assay to study the mechanisms of human blood-nerve barrier (BNB) formation and ascertain the downstream signalling pathway involved in the process. To determine the molecular changes that may occur at human BNB intercellular junctions under the influence of glial-derived neurotrophic factor (GDNF).

Mon Apr 01 00:00:00 EDT 2019

GDNF enhances human blood-nerve barrier function in vitro via MAPK signaling pathways.

Tissue barriers

Dong C,Ubogu EE

Mon Apr 01 00:00:00 EDT 2019

Acylcarnitines as markers of exercise-associated fuel partitioning, xenometabolism, and potential signals to muscle afferent neurons.

Experimental physiology

Zhang J,Light AR,Hoppel CL,Campbell C,Chandler CJ,Burnett DJ,Souza EC,Casazza GA,Hughen RW,Keim NL,Newman JW,Hunter GR,Fernandez JR,Garvey WT,Harper ME,Fiehn O,Adams SH

450-10 was used in Sample Preparation to investigate mitochondrial fatty acid oxidation in obese women, and insulin sensitivity.

Sun Jan 01 00:00:00 EST 2017

Live Imaging Mitochondrial Transport in Neurons.

Neuromethods

Course MM,Hsieh CH,Tsai PI,Codding-Bui JA,Shaltouki A,Wang X

450-10 was used in In vitro experiments to demonstrate imaging techniques to visualise mitochondrial transport system in neuronal networks, for physiological and pathological study applications.

Thu Oct 01 00:00:00 EDT 2020

Potent and Selective BACE-1 Peptide Inhibitors Lower Brain Aβ Levels Mediated by Brain Shuttle Transport.

EBioMedicine

Ruderisch N,Schlatter D,Kuglstatter A,Guba W,Huber S,Cusulin C,Benz J,Rufer AC,Hoernschemeyer J,Schweitzer C,Bülau T,Gärtner A,Hoffmann E,Niewoehner J,Patsch C,Baumann K,Loetscher H,Kitas E,Freskgård PO

450-10 was used in Cell Culture to demonstrate that the Brain Shuttle is essential for BACE1 peptide inhibitors to be efficacious in the brain and active-exosite design of BACE1 peptide inhibitors may be of therapeutic relevance.

Sun Oct 01 00:00:00 EDT 2017

PSEN1 Mutant iPSC-Derived Model Reveals Severe Astrocyte Pathology in Alzheimer's Disease.

Stem cell reports

Oksanen M,Petersen AJ,Naumenko N,Puttonen K,Lehtonen Š,Gubert Olivé M,Shakirzyanova A,Leskelä S,Sarajärvi T,Viitanen M,Rinne JO,Hiltunen M,Haapasalo A,Giniatullin R,Tavi P,Zhang SC,Kanninen KM,Hämäläinen RH,Koistinaho J

Tue Dec 12 00:00:00 EST 2017

Small molecule-based lineage switch of human adipose-derived stem cells into neural stem cells and functional GABAergic neurons.

Scientific reports

Park J,Lee N,Lee J,Choe EK,Kim MK,Lee J,Byun MS,Chon MW,Kim SW,Lee CJ,Kim JH,Kwon JS,Chang MS

Thu Aug 31 00:00:00 EDT 2017

A post-transcriptional program coordinated by CSDE1 prevents intrinsic neural differentiation of human embryonic stem cells.

Nature communications

Ju Lee H,Bartsch D,Xiao C,Guerrero S,Ahuja G,Schindler C,Moresco JJ,Yates JR,Gebauer F,Bazzi H,Dieterich C,Kurian L,Vilchez D

Mon Nov 13 00:00:00 EST 2017

Cell-type Dependent Alzheimer's Disease Phenotypes: Probing the Biology of Selective Neuronal Vulnerability.

Stem cell reports

Muratore CR,Zhou C,Liao M,Fernandez MA,Taylor WM,Lagomarsino VN,Pearse RV,Rice HC,Negri JM,He A,Srikanth P,Callahan DG,Shin T,Zhou M,Bennett DA,Noggle S,Love JC,Selkoe DJ,Young-Pearse TL

Tue Dec 12 00:00:00 EST 2017

Transcriptional signatures of schizophrenia in hiPSC-derived NPCs and neurons are concordant with post-mortem adult brains.

Nature communications

Hoffman GE,Hartley BJ,Flaherty E,Ladran I,Gochman P,Ruderfer DM,Stahl EA,Rapoport J,Sklar P,Brennand KJ

Wed Dec 20 00:00:00 EST 2017

HDAC6 inhibition reverses axonal transport defects in motor neurons derived from FUS-ALS patients.

Nature communications

Guo W,Naujock M,Fumagalli L,Vandoorne T,Baatsen P,Boon R,Ordovás L,Patel A,Welters M,Vanwelden T,Geens N,Tricot T,Benoy V,Steyaert J,Lefebvre-Omar C,Boesmans W,Jarpe M,Sterneckert J,Wegner F,Petri S,Bohl D,Vanden Berghe P,Robberecht W,Van Damme P,Verfaillie C,Van Den Bosch L

Wed Oct 11 00:00:00 EDT 2017

On-demand optogenetic activation of human stem-cell-derived neurons.

Scientific reports

Klapper SD,Sauter EJ,Swiersy A,Hyman MAE,Bamann C,Bamberg E,Busskamp V

Tue Oct 31 00:00:00 EDT 2017

On-demand optogenetic activation of human stem-cell-derived neurons.

Scientific reports

Klapper SD,Sauter EJ,Swiersy A,Hyman MAE,Bamann C,Bamberg E,Busskamp V

Tue Oct 31 00:00:00 EDT 2017

Derivation of Human Midbrain-Specific Organoids from Neuroepithelial Stem Cells.

Stem cell reports

Monzel AS,Smits LM,Hemmer K,Hachi S,Moreno EL,van Wuellen T,Jarazo J,Walter J,Brüggemann I,Boussaad I,Berger E,Fleming RMT,Bolognin S,Schwamborn JC

Tue May 09 00:00:00 EDT 2017

Reactive Astrocytes Promote ALS-like Degeneration and Intracellular Protein Aggregation in Human Motor Neurons by Disrupting Autophagy through TGF-β1.

Stem cell reports

Tripathi P,Rodriguez-Muela N,Klim JR,de Boer AS,Agrawal S,Sandoe J,Lopes CS,Ogliari KS,Williams LA,Shear M,Rubin LL,Eggan K,Zhou Q

Tue Aug 08 00:00:00 EDT 2017

GC/MS-based Analysis of Volatile Metabolic Profile Along in vitro Differentiation of Human Induced Pluripotent Stem Cells.

Bio-protocol

Capuano R,Talarico RV,Spitalieri P,Paolesse R,Novelli G,Sangiuolo F,Di Natale C

450-10 was used in Cell Culture to provide a protocol for volatile organic compounds (VOCs) analysis from human induced pluripotent stem cells (hiPSCs).

Tue Dec 05 00:00:00 EST 2017

Intertwining DNA-RNA nanocapsules loaded with tumor neoantigens as synergistic nanovaccines for cancer immunotherapy.

Nature communications

Zhu G,Mei L,Vishwasrao HD,Jacobson O,Wang Z,Liu Y,Yung BC,Fu X,Jin A,Niu G,Wang Q,Zhang F,Shroff H,Chen X

Tue Nov 14 00:00:00 EST 2017

Differential Maturation of the Two Regulated Secretory Pathways in Human iPSC-Derived Neurons.

Stem cell reports

Emperador Melero J,Nadadhur AG,Schut D,Weering JV,Heine VM,Toonen RF,Verhage M

Tue Mar 14 00:00:00 EDT 2017

Two approaches reveal a new paradigm of 'switchable or genetics-influenced allele-specific DNA methylation' with potential in human disease.

Cell discovery

Martos SN,Li T,Ramos RB,Lou D,Dai H,Xu JC,Gao G,Gao Y,Wang Q,An C,Zhang X,Jia Y,Dawson VL,Dawson TM,Ji H,Wang Z

Tue Sep 29 00:00:00 EDT 2020

Capturing the biology of disease severity in a PSC-based model of familial dysautonomia.

Nature medicine

Zeltner N,Fattahi F,Dubois NC,Saurat N,Lafaille F,Shang L,Zimmer B,Tchieu J,Soliman MA,Lee G,Casanova JL,Studer L

450-10 was used in Cell Culture to study disease-related phenotypes in human pluripotent stem cells (PSCs) that capture familial dysautonomia (FD) severity and demonstrate that PSC-based modeling is sensitive in recapitulating disease severity, which presents an important step toward personalized medicine.

Thu Dec 01 00:00:00 EST 2016

Human neural crest cells contribute to coat pigmentation in interspecies chimeras after in utero injection into mouse embryos.

Proceedings of the National Academy of Sciences of the United States of America

Cohen MA,Wert KJ,Goldmann J,Markoulaki S,Buganim Y,Fu D,Jaenisch R

Tue Feb 09 00:00:00 EST 2016

Generation of highly enriched V2a interneurons from mouse embryonic stem cells.

Experimental neurology

Iyer NR,Huettner JE,Butts JC,Brown CR,Sakiyama-Elbert SE

450-10 was used in Cell Culture to provide a unique tool to begin studying V2a INs in isolation or for use in in vitro models of spinal microcircuits.

Tue Mar 01 00:00:00 EST 2016

TGFβ signaling regulates the choice between pluripotent and neural fates during reprogramming of human urine derived cells.

Scientific reports

Wang L,Li X,Huang W,Zhou T,Wang H,Lin A,Hutchins AP,Su Z,Chen Q,Pei D,Pan G

Thu Mar 03 00:00:00 EST 2016

The Hippo pathway member YAP enhances human neural crest cell fate and migration.

Scientific reports

Hindley CJ,Condurat AL,Menon V,Thomas R,Azmitia LM,Davis JA,Pruszak J

Wed Mar 16 00:00:00 EDT 2016

Dual-SMAD Inhibition/WNT Activation-Based Methods to Induce Neural Crest and Derivatives from Human Pluripotent Stem Cells.

Methods in molecular biology (Clifton, N.J.)

Chambers SM,Mica Y,Lee G,Studer L,Tomishima MJ

450-10 was used in In vitro experiments to provide methods to direct NC into two different terminal fates: melanocytes and sensory neurons.

Mon May 16 00:00:00 EDT 2016

Human GV oocytes generated by mitotically active germ cells obtained from follicular aspirates.

Scientific reports

Ding X,Liu G,Xu B,Wu C,Hui N,Ni X,Wang J,Du M,Teng X,Wu J

Thu Jun 30 00:00:00 EDT 2016

Human GV oocytes generated by mitotically active germ cells obtained from follicular aspirates.

Scientific reports

Ding X,Liu G,Xu B,Wu C,Hui N,Ni X,Wang J,Du M,Teng X,Wu J

Thu Jun 30 00:00:00 EDT 2016

Distinct and shared functions of ALS-associated proteins TDP-43, FUS and TAF15 revealed by multisystem analyses.

Nature communications

Kapeli K,Pratt GA,Vu AQ,Hutt KR,Martinez FJ,Sundararaman B,Batra R,Freese P,Lambert NJ,Huelga SC,Chun SJ,Liang TY,Chang J,Donohue JP,Shiue L,Zhang J,Zhu H,Cambi F,Kasarskis E,Hoon S,Ares M,Burge CB,Ravits J,Rigo F,Yeo GW

Tue Jul 05 00:00:00 EDT 2016

5-HT2 receptors mediate functional modulation of GABAa receptors and inhibitory synaptic transmissions in human iPS-derived neurons.

Scientific reports

Wang H,Hu L,Liu C,Su Z,Wang L,Pan G,Guo Y,He J

Wed Feb 03 00:00:00 EST 2016

Fyn Kinase regulates GluN2B subunit-dominant NMDA receptors in human induced pluripotent stem cell-derived neurons.

Scientific reports

Zhang WB,Ross PJ,Tu Y,Wang Y,Beggs S,Sengar AS,Ellis J,Salter MW

Mon Apr 04 00:00:00 EDT 2016

BMS-708163 and Nilotinib restore synaptic dysfunction in human embryonic stem cell-derived Alzheimer's disease models.

Scientific reports

Nishioka H,Tooi N,Isobe T,Nakatsuji N,Aiba K

Mon Sep 19 00:00:00 EDT 2016

Human somatic cells subjected to genetic induction with six germ line-related factors display meiotic germ cell-like features.

Scientific reports

Medrano JV,Martínez-Arroyo AM,Míguez JM,Moreno I,Martínez S,Quiñonero A,Díaz-Gimeno P,Marqués-Marí AI,Pellicer A,Remohí J,Simón C

Tue Apr 26 00:00:00 EDT 2016

A painful neuropathy-associated Nav1.7 mutant leads to time-dependent degeneration of small-diameter axons associated with intracellular Ca2+ dysregulation and decrease in ATP levels.

Molecular pain

Rolyan H,Liu S,Hoeijmakers JG,Faber CG,Merkies IS,Lauria G,Black JA,Waxman SG

450-10 was used in Cell Culture to investigate the role of degeneration of neurites using Nav1.7 mutants in dorsal root ganglia neurons.

Wed Oct 18 00:00:00 EDT 2017

Rapid Ngn2-induction of excitatory neurons from hiPSC-derived neural progenitor cells.

Methods (San Diego, Calif.)

Ho SM,Hartley BJ,Tcw J,Beaumont M,Stafford K,Slesinger PA,Brennand KJ

450-10 was used in Cell Culture to provide an adapted protocol that accelerates maturation of functional excitatory neurons from human induced pluripotent stem cell (hiPSC)-derived neural progenitor cells (NPCs) via lentiviral transduction of Neurogenin 2 (using both mNgn2 and hNGN2) which could be readily amenable to scaling for hiPSC-based high-throughput drug screening.

Sun May 15 00:00:00 EDT 2016

Generation and transplantation of reprogrammed human neurons in the brain using 3D microtopographic scaffolds.

Nature communications

Carlson AL,Bennett NK,Francis NL,Halikere A,Clarke S,Moore JC,Hart RP,Paradiso K,Wernig M,Kohn J,Pang ZP,Moghe PV

Thu Mar 17 00:00:00 EDT 2016

Generation and transplantation of reprogrammed human neurons in the brain using 3D microtopographic scaffolds.

Nature communications

Carlson AL,Bennett NK,Francis NL,Halikere A,Clarke S,Moore JC,Hart RP,Paradiso K,Wernig M,Kohn J,Pang ZP,Moghe PV

Thu Mar 17 00:00:00 EDT 2016

FolR1: a novel cell surface marker for isolating midbrain dopamine neural progenitors and nascent dopamine neurons.

Scientific reports

Gennet N,Tamburini C,Nan X,Li M

Thu Sep 01 00:00:00 EDT 2016

Differential neuronal vulnerability identifies IGF-2 as a protective factor in ALS.

Scientific reports

Allodi I,Comley L,Nichterwitz S,Nizzardo M,Simone C,Benitez JA,Cao M,Corti S,Hedlund E

Mon May 16 00:00:00 EDT 2016

Somatic increase of CCT8 mimics proteostasis of human pluripotent stem cells and extends C. elegans lifespan.

Nature communications

Noormohammadi A,Khodakarami A,Gutierrez-Garcia R,Lee HJ,Koyuncu S,König T,Schindler C,Saez I,Fatima A,Dieterich C,Vilchez D

Mon Nov 28 00:00:00 EST 2016

Cell type-dependent Erk-Akt pathway crosstalk regulates the proliferation of fetal neural progenitor cells.

Scientific reports

Rhim JH,Luo X,Gao D,Xu X,Zhou T,Li F,Wang P,Wong ST,Xia X

Mon May 23 00:00:00 EDT 2016

Morpholino-mediated SOD1 reduction ameliorates an amyotrophic lateral sclerosis disease phenotype.

Scientific reports

Nizzardo M,Simone C,Rizzo F,Ulzi G,Ramirez A,Rizzuti M,Bordoni A,Bucchia M,Gatti S,Bresolin N,Comi GP,Corti S

Tue Feb 16 00:00:00 EST 2016

Dopaminergic neurons differentiating from LRRK2 G2019S induced pluripotent stem cells show early neuritic branching defects.

Scientific reports

Borgs L,Peyre E,Alix P,Hanon K,Grobarczyk B,Godin JD,Purnelle A,Krusy N,Maquet P,Lefebvre P,Seutin V,Malgrange B,Nguyen L

Mon Sep 19 00:00:00 EDT 2016

Neuronal medium that supports basic synaptic functions and activity of human neurons in vitro.

Proceedings of the National Academy of Sciences of the United States of America

Bardy C,van den Hurk M,Eames T,Marchand C,Hernandez RV,Kellogg M,Gorris M,Galet B,Palomares V,Brown J,Bang AG,Mertens J,Böhnke L,Boyer L,Simon S,Gage FH

Tue May 19 00:00:00 EDT 2015

Notch signaling regulates motor neuron differentiation of human embryonic stem cells.

Stem cells (Dayton, Ohio)

Ben-Shushan E,Feldman E,Reubinoff BE

Sun Feb 01 00:00:00 EST 2015

PTEN deficiency reprogrammes human neural stem cells towards a glioblastoma stem cell-like phenotype.

Nature communications

Duan S,Yuan G,Liu X,Ren R,Li J,Zhang W,Wu J,Xu X,Fu L,Li Y,Yang J,Zhang W,Bai R,Yi F,Suzuki K,Gao H,Esteban CR,Zhang C,Izpisua Belmonte JC,Chen Z,Wang X,Jiang T,Qu J,Tang F,Liu GH

Thu Dec 03 00:00:00 EST 2015

Rapid and robust generation of long-term self-renewing human neural stem cells with the ability to generate mature astroglia.

Scientific reports

Palm T,Bolognin S,Meiser J,Nickels S,Träger C,Meilenbrock RL,Brockhaus J,Schreitmüller M,Missler M,Schwamborn JC

Fri Nov 06 00:00:00 EST 2015

A guide to generating and using hiPSC derived NPCs for the study of neurological diseases.

Journal of visualized experiments : JoVE

Topol A,Tran NN,Brennand KJ

450-10 was used in Sample Preparation to study a methos for the efficient differentiation of human induced pluripotent stem cells (hiPSCs) into neural progenitor cells (NPCs) and neurons for the study of these diseases, it is now possible to recapitulate the developmental events that occurred prior to symptom onset in patients.

Sat Feb 21 00:00:00 EST 2015

Spinal muscular atrophy phenotype is ameliorated in human motor neurons by SMN increase via different novel RNA therapeutic approaches.

Scientific reports

Nizzardo M,Simone C,Dametti S,Salani S,Ulzi G,Pagliarani S,Rizzo F,Frattini E,Pagani F,Bresolin N,Comi G,Corti S

Tue Jun 30 00:00:00 EDT 2015

Elevated α-synuclein caused by SNCA gene triplication impairs neuronal differentiation and maturation in Parkinson's patient-derived induced pluripotent stem cells.

Cell death & disease

Oliveira LM,Falomir-Lockhart LJ,Botelho MG,Lin KH,Wales P,Koch JC,Gerhardt E,Taschenberger H,Outeiro TF,Lingor P,Schüle B,Arndt-Jovin DJ,Jovin TM

Thu Nov 26 00:00:00 EST 2015

Differentiation of human ESCs to retinal ganglion cells using a CRISPR engineered reporter cell line.

Scientific reports

Sluch VM,Davis CH,Ranganathan V,Kerr JM,Krick K,Martin R,Berlinicke CA,Marsh-Armstrong N,Diamond JS,Mao HQ,Zack DJ

Fri Nov 13 00:00:00 EST 2015

Selective Generation of Dopaminergic Precursors from Mouse Fibroblasts by Direct Lineage Conversion.

Scientific reports

Tian C,Li Y,Huang Y,Wang Y,Chen D,Liu J,Deng X,Sun L,Anderson K,Qi X,Li Y,Lee Mosley R,Chen X,Huang J,Zheng JC

Thu Jul 30 00:00:00 EDT 2015

APP intracellular domain acts as a transcriptional regulator of miR-663 suppressing neuronal differentiation.

Cell death & disease

Shu R,Wong W,Ma QH,Yang ZZ,Zhu H,Liu FJ,Wang P,Ma J,Yan S,Polo JM,Bernard CC,Stanton LW,Dawe GS,Xiao ZC

Thu Feb 19 00:00:00 EST 2015

Foxp1-mediated programming of limb-innervating motor neurons from mouse and human embryonic stem cells.

Nature communications

Adams KL,Rousso DL,Umbach JA,Novitch BG

Tue Apr 14 00:00:00 EDT 2015

Foxp1-mediated programming of limb-innervating motor neurons from mouse and human embryonic stem cells.

Nature communications

Adams KL,Rousso DL,Umbach JA,Novitch BG

Tue Apr 14 00:00:00 EDT 2015

Brain ischemia downregulates the neuroprotective GDNF-Ret signaling by a calpain-dependent mechanism in cultured hippocampal neurons.

Cell death & disease

Curcio M,Salazar IL,Inácio AR,Duarte EP,Canzoniero LM,Duarte CB

Thu Feb 12 00:00:00 EST 2015

Deterministic HOX patterning in human pluripotent stem cell-derived neuroectoderm.

Stem cell reports

Lippmann ES,Williams CE,Ruhl DA,Estevez-Silva MC,Chapman ER,Coon JJ,Ashton RS

Tue Apr 14 00:00:00 EDT 2015

Cell cycle and p53 gate the direct conversion of human fibroblasts to dopaminergic neurons.

Nature communications

Jiang H,Xu Z,Zhong P,Ren Y,Liang G,Schilling HA,Hu Z,Zhang Y,Wang X,Chen S,Yan Z,Feng J

Mon Dec 07 00:00:00 EST 2015

Dysregulation of gene expression as a cause of Cockayne syndrome neurological disease.

Proceedings of the National Academy of Sciences of the United States of America

Wang Y,Chakravarty P,Ranes M,Kelly G,Brooks PJ,Neilan E,Stewart A,Schiavo G,Svejstrup JQ

Tue Oct 07 00:00:00 EDT 2014

Treating small fiber neuropathy by topical application of a small molecule modulator of ligand-induced GFRα/RET receptor signaling.

Proceedings of the National Academy of Sciences of the United States of America

Hedstrom KL,Murtie JC,Albers K,Calcutt NA,Corfas G

Tue Feb 11 00:00:00 EST 2014

Defined human pluripotent stem cell culture enables highly efficient neuroepithelium derivation without small molecule inhibitors.

Stem cells (Dayton, Ohio)

Lippmann ES,Estevez-Silva MC,Ashton RS

Tue Apr 01 00:00:00 EDT 2014

Efficient specification of interneurons from human pluripotent stem cells by dorsoventral and rostrocaudal modulation.

Stem cells (Dayton, Ohio)

Kim TG,Yao R,Monnell T,Cho JH,Vasudevan A,Koh A,Peeyush KT,Moon M,Datta D,Bolshakov VY,Kim KS,Chung S

Tue Jul 01 00:00:00 EDT 2014

Role of astroglia in Down's syndrome revealed by patient-derived human-induced pluripotent stem cells.

Nature communications

Chen C,Jiang P,Xue H,Peterson SE,Tran HT,McCann AE,Parast MM,Li S,Pleasure DE,Laurent LC,Loring JF,Liu Y,Deng W

Fri Jul 18 00:00:00 EDT 2014

Direct conversion of fibroblasts to neurons by reprogramming PTB-regulated microRNA circuits.

Cell

Xue Y,Ouyang K,Huang J,Zhou Y,Ouyang H,Li H,Wang G,Wu Q,Wei C,Bi Y,Jiang L,Cai Z,Sun H,Zhang K,Zhang Y,Chen J,Fu XD

Thu Jan 17 00:00:00 EST 2013

Direct conversion of fibroblasts to neurons by reprogramming PTB-regulated microRNA circuits.

Cell

Xue Y,Ouyang K,Huang J,Zhou Y,Ouyang H,Li H,Wang G,Wu Q,Wei C,Bi Y,Jiang L,Cai Z,Sun H,Zhang K,Zhang Y,Chen J,Fu XD

Thu Jan 17 00:00:00 EST 2013

Autologous mesenchymal stem cell-derived dopaminergic neurons function in parkinsonian macaques.

The Journal of clinical investigation

Hayashi T,Wakao S,Kitada M,Ose T,Watabe H,Kuroda Y,Mitsunaga K,Matsuse D,Shigemoto T,Ito A,Ikeda H,Fukuyama H,Onoe H,Tabata Y,Dezawa M

Tue Jan 01 00:00:00 EST 2013

Improved cell therapy protocols for Parkinson's disease based on differentiation efficiency and safety of hESC-, hiPSC-, and non-human primate iPSC-derived dopaminergic neurons.

Stem cells (Dayton, Ohio)

Sundberg M,Bogetofte H,Lawson T,Jansson J,Smith G,Astradsson A,Moore M,Osborn T,Cooper O,Spealman R,Hallett P,Isacson O

Thu Aug 01 00:00:00 EDT 2013

Improved cell therapy protocols for Parkinson's disease based on differentiation efficiency and safety of hESC-, hiPSC-, and non-human primate iPSC-derived dopaminergic neurons.

Stem cells (Dayton, Ohio)

Sundberg M,Bogetofte H,Lawson T,Jansson J,Smith G,Astradsson A,Moore M,Osborn T,Cooper O,Spealman R,Hallett P,Isacson O

Thu Aug 01 00:00:00 EDT 2013

Increased proteasome activity in human embryonic stem cells is regulated by PSMD11.

Nature

Vilchez D,Boyer L,Morantte I,Lutz M,Merkwirth C,Joyce D,Spencer B,Page L,Masliah E,Berggren WT,Gage FH,Dillin A

Thu Sep 13 00:00:00 EDT 2012

Cord blood-derived neuronal cells by ectopic expression of Sox2 and c-Myc.

Proceedings of the National Academy of Sciences of the United States of America

Giorgetti A,Marchetto MC,Li M,Yu D,Fazzina R,Mu Y,Adamo A,Paramonov I,Cardoso JC,Monasterio MB,Bardy C,Cassiani-Ingoni R,Liu GH,Gage FH,Izpisua Belmonte JC

Tue Jul 31 00:00:00 EDT 2012

Increasing doublecortin expression promotes migration of human embryonic stem cell-derived neurons.

Stem cells (Dayton, Ohio)

Filipovic R,Santhosh Kumar S,Fiondella C,Loturco J

Sat Sep 01 00:00:00 EDT 2012

Kinesin light chain 1 suppression impairs human embryonic stem cell neural differentiation and amyloid precursor protein metabolism.

PloS one

Killian RL,Flippin JD,Herrera CM,Almenar-Queralt A,Goldstein LS

Mon Jun 11 00:00:00 EDT 2012

Highly pure and expandable PSA-NCAM-positive neural precursors from human ESC and iPSC-derived neural rosettes.

PloS one

Kim DS,Lee DR,Kim HS,Yoo JE,Jung SJ,Lim BY,Jang J,Kang HC,You S,Hwang DY,Leem JW,Nam TS,Cho SR,Kim DW

Fri Apr 19 00:00:00 EDT 2013

Kinome-wide selectivity profiling of ATP-competitive mammalian target of rapamycin (mTOR) inhibitors and characterization of their binding kinetics.

The Journal of biological chemistry

Liu Q,Kirubakaran S,Hur W,Niepel M,Westover K,Thoreen CC,Wang J,Ni J,Patricelli MP,Vogel K,Riddle S,Waller DL,Traynor R,Sanda T,Zhao Z,Kang SA,Zhao J,Look AT,Sorger PK,Sabatini DM,Gray NS

Fri Mar 23 00:00:00 EDT 2012

Hedgehog/Notch-induced premature gliogenesis represents a new disease mechanism for Hirschsprung disease in mice and humans.

The Journal of clinical investigation

Ngan ES,Garcia-Barceló MM,Yip BH,Poon HC,Lau ST,Kwok CK,Sat E,Sham MH,Wong KK,Wainwright BJ,Cherny SS,Hui CC,Sham PC,Lui VC,Tam PK

Thu Sep 01 00:00:00 EDT 2011

Efficient derivation of NPCs, spinal motor neurons and midbrain dopaminergic neurons from hESCs at 3% oxygen.

Nature protocols

Stacpoole SR,Bilican B,Webber DJ,Luzhynskaya A,He XL,Compston A,Karadottir R,Franklin RJ,Chandran S

Thu Jul 28 00:00:00 EDT 2011

Ataxia telangiectasia mutated (ATM) modulates long interspersed element-1 (L1) retrotransposition in human neural stem cells.

Proceedings of the National Academy of Sciences of the United States of America

Coufal NG,Garcia-Perez JL,Peng GE,Marchetto MC,Muotri AR,Mu Y,Carson CT,Macia A,Moran JV,Gage FH

Tue Dec 20 00:00:00 EST 2011

Heparan sulfate proteoglycan syndecan-3 is a novel receptor for GDNF, neurturin, and artemin.

The Journal of cell biology

Bespalov MM,Sidorova YA,Tumova S,Ahonen-Bishopp A,Magalhães AC,Kulesskiy E,Paveliev M,Rivera C,Rauvala H,Saarma M

Mon Jan 10 00:00:00 EST 2011

Heparan sulfate proteoglycan syndecan-3 is a novel receptor for GDNF, neurturin, and artemin.

The Journal of cell biology

Bespalov MM,Sidorova YA,Tumova S,Ahonen-Bishopp A,Magalhães AC,Kulesskiy E,Paveliev M,Rivera C,Rauvala H,Saarma M

Mon Jan 10 00:00:00 EST 2011

Heparan sulfate proteoglycan syndecan-3 is a novel receptor for GDNF, neurturin, and artemin.

The Journal of cell biology

Bespalov MM,Sidorova YA,Tumova S,Ahonen-Bishopp A,Magalhães AC,Kulesskiy E,Paveliev M,Rivera C,Rauvala H,Saarma M

Mon Jan 10 00:00:00 EST 2011

Isolation of MECP2-null Rett Syndrome patient hiPS cells and isogenic controls through X-chromosome inactivation.

Human molecular genetics

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Wed Jun 01 00:00:00 EDT 2011

Downregulation of VAPB expression in motor neurons derived from induced pluripotent stem cells of ALS8 patients.

Human molecular genetics

Mitne-Neto M,Machado-Costa M,Marchetto MC,Bengtson MH,Joazeiro CA,Tsuda H,Bellen HJ,Silva HC,Oliveira AS,Lazar M,Muotri AR,Zatz M

Thu Sep 15 00:00:00 EDT 2011

Mesencephalic astrocyte-derived neurotrophic factor (MANF) has a unique mechanism to rescue apoptotic neurons.

The Journal of biological chemistry

Hellman M,Arumäe U,Yu LY,Lindholm P,Peränen J,Saarma M,Permi P

Fri Jan 28 00:00:00 EST 2011

Cell-surface marker signatures for the isolation of neural stem cells, glia and neurons derived from human pluripotent stem cells.

PloS one

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Wed Mar 02 00:00:00 EST 2011

Expression of PROKR1 and PROKR2 in human enteric neural precursor cells and identification of sequence variants suggest a role in HSCR.

PloS one

Ruiz-Ferrer M,Torroglosa A,Núñez-Torres R,de Agustín JC,Antiñolo G,Borrego S

Wed Feb 15 00:00:00 EST 2012

Parkinson's disease induced pluripotent stem cells with triplication of the α-synuclein locus.

Nature communications

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Tue Aug 23 00:00:00 EDT 2011

Directed differentiation of dopamine neurons from human pluripotent stem cells.

Methods in molecular biology (Clifton, N.J.)

Ma L,Liu Y,Zhang SC

450-10 was used in Cell Culture to describe a chemically defined protocol for mDA neuron differentiation.

Thu Nov 24 00:00:00 EST 2011

Cyclical and patch-like GDNF distribution along the basal surface of Sertoli cells in mouse and hamster testes.

PloS one

Sato T,Aiyama Y,Ishii-Inagaki M,Hara K,Tsunekawa N,Harikae K,Uemura-Kamata M,Shinomura M,Zhu XB,Maeda S,Kuwahara-Otani S,Kudo A,Kawakami H,Kanai-Azuma M,Fujiwara M,Miyamae Y,Yoshida S,Seki M,Kurohmaru M,Kanai Y

Mon Apr 23 00:00:00 EDT 2012

Ret-dependent and Ret-independent mechanisms of Gfl-induced sensitization.

Molecular pain

Schmutzler BS,Roy S,Pittman SK,Meadows RM,Hingtgen CM

Wed Mar 30 00:00:00 EDT 2011

Specification of region-specific neurons including forebrain glutamatergic neurons from human induced pluripotent stem cells.

PloS one

Zeng H,Guo M,Martins-Taylor K,Wang X,Zhang Z,Park JW,Zhan S,Kronenberg MS,Lichtler A,Liu HX,Chen FP,Yue L,Li XJ,Xu RH

Thu Jul 29 00:00:00 EDT 2010

Protease activated receptors 1 and 4 sensitize TRPV1 in nociceptive neurones.

Molecular pain

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Mon Sep 27 00:00:00 EDT 2010

High-efficiency transfection of cultured primary motor neurons to study protein localization, trafficking, and function.

Molecular neurodegeneration

Fallini C,Bassell GJ,Rossoll W

Wed Apr 21 00:00:00 EDT 2010

Quantitative automated microscopy (QuAM) elucidates growth factor specific signalling in pain sensitization.

Molecular pain

Andres C,Meyer S,Dina OA,Levine JD,Hucho T

Mon Dec 27 00:00:00 EST 2010

Normalization with genes encoding ribosomal proteins but not GAPDH provides an accurate quantification of gene expressions in neuronal differentiation of PC12 cells.

BMC genomics

Zhou L,Lim QE,Wan G,Too HP

Fri Jan 29 00:00:00 EST 2010

High-efficient generation of induced pluripotent stem cells from human astrocytes.

PloS one

Ruiz S,Brennand K,Panopoulos AD,Herrerías A,Gage FH,Izpisua-Belmonte JC

Thu Dec 09 00:00:00 EST 2010

Induced pluripotent stem cells from a spinal muscular atrophy patient.

Nature

Ebert AD,Yu J,Rose FF,Mattis VB,Lorson CL,Thomson JA,Svendsen CN

Thu Jan 15 00:00:00 EST 2009

Differentiation of spinal motor neurons from pluripotent human stem cells.

Nature protocols

Hu BY,Zhang SC

Mon Oct 19 00:00:00 EDT 2009

Practical induction system for dopamine-producing cells from bone marrow stromal cells using spermine-pullulan-mediated reverse transfection method.

Tissue engineering. Part A

Nagane K,Kitada M,Wakao S,Dezawa M,Tabata Y

Wed Jul 01 00:00:00 EDT 2009

Efficient derivation of functional dopaminergic neurons from human embryonic stem cells on a large scale.

Nature protocols

Cho MS,Hwang DY,Kim DW

Thu Jan 22 00:00:00 EST 2009

Effects of NGF, NT-3 and GDNF family members on neurite outgrowth and migration from pelvic ganglia from embryonic and newborn mice.

BMC developmental biology

Stewart AL,Anderson RB,Kobayashi K,Young HM

Fri Jul 25 00:00:00 EDT 2008

Striatal delivery of neurturin by CERE-120, an AAV2 vector for the treatment of dopaminergic neuron degeneration in Parkinson's disease.

Molecular therapy : the journal of the American Society of Gene Therapy

Gasmi M,Herzog CD,Brandon EP,Cunningham JJ,Ramirez GA,Ketchum ET,Bartus RT

Mon Jan 01 00:00:00 EST 2007

Fgfr2b mediated epithelial-mesenchymal interactions coordinate tooth morphogenesis and dental trigeminal axon patterning.

Mechanisms of development

Kettunen P,Spencer-Dene B,Furmanek T,Kvinnsland IH,Dickson C,Thesleff I,Luukko K

Wed Mar 19 00:00:00 EDT 2008

Protection by GDNF and other trophic factors against the dopamine-depleting effects of neurotoxic doses of methamphetamine.

Annals of the New York Academy of Sciences

Cass WA,Peters LE,Harned ME,Seroogy KB

Tue Aug 01 00:00:00 EDT 2006

Synaptic targeting of retrogradely transported trophic factors in motoneurons: comparison of glial cell line-derived neurotrophic factor, brain-derived neurotrophic factor, and cardiotrophin-1 with tetanus toxin.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Rind HB,Butowt R,von Bartheld CS

Wed Jan 19 00:00:00 EST 2005

Normal biogenesis and cycling of empty synaptic vesicles in dopamine neurons of vesicular monoamine transporter 2 knockout mice.

Molecular biology of the cell

Croft BG,Fortin GD,Corera AT,Edwards RH,Beaudet A,Trudeau LE,Fon EA

450-10 was used in Cell Culture to suggest that physiological changes in VMAT2 levels or trafficking at the synapse may regulate dopamine release by altering the ratio of fillable-to-empty synaptic vesicles, as both continue to cycle in response to neural activity.

Sat Jan 01 00:00:00 EST 2005

GDNF family ligands activate multiple events during axonal growth in mature sensory neurons.

Molecular and cellular neurosciences

Paveliev M,Airaksinen MS,Saarma M

Mon Mar 01 00:00:00 EST 2004

Retarded growth and deficits in the enteric and parasympathetic nervous system in mice lacking GFR alpha2, a functional neurturin receptor.

Neuron

Rossi J,Luukko K,Poteryaev D,Laurikainen A,Sun YF,Laakso T,Eerikäinen S,Tuominen R,Lakso M,Rauvala H,Arumäe U,Pasternack M,Saarma M,Airaksinen MS

Mon Feb 01 00:00:00 EST 1999

Transplantation of expanded mesencephalic precursors leads to recovery in parkinsonian rats.

Nature neuroscience

Studer L,Tabar V,McKay RD

Sat Aug 01 00:00:00 EDT 1998

Cyclic AMP elevation is sufficient to promote the survival of spinal motor neurons in vitro.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Hanson MG,Shen S,Wiemelt AP,McMorris FA,Barres BA

Tue Sep 15 00:00:00 EDT 1998

Regulation of quantal secretion by neurotrophic factors at developing motoneurons in Xenopus cell cultures.

The Journal of physiology

Liou JC,Yang RS,Fu WM

Fri Aug 15 00:00:00 EDT 1997

Bioinformatics

Protein Aliases: Astrocyte-derived trophic factor; ATF; Glial cell line-derived neurotrophic factor; H-GDNF; hGDNF

Human GDNF Recombinant Protein, PeproTech®

Human GDNF Protein (450-10-10UG) in Functional

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450-10-10UG

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