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Product Details
300-71-25UG
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
KRRPAKAWSG RRTRLCCHRV PSPNSTNLKG HHVRLCKPCK LEPEPRLWVV PGALPQV
Molecular weight
6.5 kDa
Class
Recombinant
Type
Protein
Purity
≥ 98% by SDS-PAGE gel and HPLC analyses.
Endotoxin concentration
<1 EU/µg
Activity
Determined by its ability to chemoattract human PBMC's in a transwell-based migration assay.
Conjugate
Form
Lyophilized
Purification
purified
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
Product Specific Information
PeproTech's E.coli-derived Recombinant Human GPR15L consists of 57 amino acid residues and has a calculated molecular weight of 6.5 kDa.
This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis
Target Information
GPR15L is a newly identified ligand for GPR15, a member of the G protein-coupled receptor (GPCR) family. Upon ligation, GPR15L acts as a potent chemoattractant for GPR15-expressing T cells and together they mediate lymphocyte recruitment to the large intestine and skin. GPR15L is constitutively expressed by colon epithelial cells where its expression is minimally altered by intestinal inflammation. Conversely, GPR15L is nearly undetectable in adult epidermis but highly upregulated during wound healing and inflammation, particularly in psoriasis. Significant expression of GPR15L is also seen in additional mucosal epithelial cells, including those of the stomach, esophagus, and urinary tract. While maintaining similar expression patterns and intramolecular disulfide cysteine bridges found in members of the CC chemokine family, GPR15L differs from classic CC and CXC chemokines, whose active sites are found on the N-terminus, in that it relies on its C-terminus for receptor interaction. GPR15L was first identified as an antimicrobial peptide (AMP) due to its broad spectrum of antimicrobial activity, a property shared with many chemokines. GPR15L binds to an additional receptor, SUSD2, and early studies have indicated that both are downregulated in colon cancer tissue resulting in inhibited colon cancer growth.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: anti-microbial peptide with 57 amino acid residues; Antimicrobial peptide with 57 amino acid residues; antimicrobial peptide-57; AP-57; Colon-derived SUSD2 binding factor; CSBF; Protein GPR15 ligand; Protein GPR15L; Protein GPR15LG; RLLV1833; Secreted protein C10orf99
Gene Aliases: AP-57; C10orf99; CSBF; GPR15L; GPR15LG; UNQ1833; UNQ1833/PRO3446
UniProt ID: (Human) Q6UWK7
Entrez Gene ID: (Human) 387695
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