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Product Details
RP-96865
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
AVRCQSQVAEAVLTSQLKAHQEKPN
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
1M urea/PBS, pH 7.4
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (73%), Rat (73%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-57474. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is an enzyme belonging to the class II family of aminoacyl-tRNA synthetases. Functioning in the synthesis of histidyl-transfer RNA, the enzyme plays an accessory role in the regulation of protein biosynthesis. The gene is located in a head-to-head orientation with HARS on chromosome five, where the homologous genes likely share a bidirectional promoter. Mutations in this gene are associated with the pathogenesis of Perrault syndrome, which involves ovarian dysgenesis and sensorineural hearing loss. Alternative splicing results in multiple transcript variants of this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EC 6.1.1.21; HARS-related; HisRS; histidine translase; histidine tRNA ligase 2, mitochondrial (putative); Histidine--tRNA ligase, mitochondrial; Histidine--tRNA ligase-like; histidine-tRNA ligase homolog; Histidyl-tRNA synthetase; histidyl-tRNA synthetase 2; histidyl-tRNA synthetase 2, mitochondrial (putative); probable histidyl-tRNA synthetase, mitochondrial
Gene Aliases: HARS2; HARSL; HARSR; HO3; PRLTS2
UniProt ID: (Human) P49590
Entrez Gene ID: (Human) 23438
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