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Human Heregulin beta-1, Animal-Free Recombinant Protein, PeproTech®

Human Heregulin beta-1, Animal-Free Protein in Functional Assay (Functional)

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Human Heregulin beta-1, Animal-Free Protein (AF-100-03-50UG) in Functional

Bioassay analysis of Human Heregulin beta-1, Animal-Free Recombinant Protein, PeproTech® (Product # AF-100-03-1MG).

Product Image Gallery

Product Details

AF-100-03-50UG

Applications

Tested Dilution

Publications

Functional Assay (Functional)

Assay-dependent

In vitro Assay (IV)

View 13 publications 13 publications

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species

Human

Published species

Human, Mouse

Expression System

E. coli

Amino acid sequence

SHLVKCAEKE KTFCVNGGEC FMVKDLSNPS RYLCKCPNEF TGDRCQNYVM ASFYKHLGIE FMEAE

Molecular weight

7.5 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<0.1 EU/µg

Activity

The ED50 was determined by the dose-dependent stimulation of the proliferation of human MCF-7 cells is ≤ 0.5 ng/ml, corresponding to a specific activity of ≥ 2 x 10^6 units/mg.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Human Heregulin beta-1 (HRG1-B1) is a 7.5 kDa polypeptide consisting of only the EGF domain of heregulin beta-1 (65 amino acid residues).

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

Direct ligand for ERBB3 and ERBB4 tyrosine kinase receptors. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. The multiple isoforms perform diverse functions such as inducing growth and differentiation of epithelial, glial, neuronal, and skeletal muscle cells; inducing expression of acetylcholine receptor in synaptic vesicles during the formation of the neuromuscular junction; stimulating lobuloalveolar budding and milk production in the mammary gland and inducing differentiation of mammary tumor cells; stimulating Schwann cell proliferation; implication in the development of the myocardium such as trabeculation of the developing heart. Isoform 10 may play a role in motor and sensory neuron development.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Targeting lactate dehydrogenase B-dependent mitochondrial metabolism affects tumor initiating cells and inhibits tumorigenesis of non-small cell lung cancer by inducing mtDNA damage.

Cellular and molecular life sciences : CMLS

Deng H,Gao Y,Trappetti V,Hertig D,Karatkevich D,Losmanova T,Urzi C,Ge H,Geest GA,Bruggmann R,Djonov V,Nuoffer JM,Vermathen P,Zamboni N,Riether C,Ochsenbein A,Peng RW,Kocher GJ,Schmid RA,Dorn P,Marti TM

AF-100-03 was used in In vitro experiments to show that LDHB is essential for the maintenance of mitochondrial metabolism, especially nucleotide metabolism, demonstrating that LDHB is crucial for the survival and proliferation of NSCLC tumor-initiating cells and tumorigenesis.

Mon Jul 25 00:00:00 EDT 2022

CODEX, a neural network approach to explore signaling dynamics landscapes.

Molecular systems biology

Jacques MA,Dobrzyński M,Gagliardi PA,Sznitman R,Pertz O

Thu Apr 01 00:00:00 EDT 2021

TMEM43-S358L mutation enhances NF-κB-TGFβ signal cascade in arrhythmogenic right ventricular dysplasia/cardiomyopathy.

Protein & cell

Zheng G,Jiang C,Li Y,Yang D,Ma Y,Zhang B,Li X,Zhang P,Hu X,Zhao X,Du J,Lin X

AF-100-03 was used in Sample Preparation (Western Blotting) to investigate the relationship of TMEM43-S358L and ARVD/C in mouse models.

Fri Feb 01 00:00:00 EST 2019

The NF2 tumor suppressor merlin interacts with Ras and RasGAP, which may modulate Ras signaling.

Oncogene

Cui Y,Groth S,Troutman S,Carlstedt A,Sperka T,Riecken LB,Kissil JL,Jin H,Morrison H

Sun Sep 01 00:00:00 EDT 2019

The NF2 tumor suppressor merlin interacts with Ras and RasGAP, which may modulate Ras signaling.

Oncogene

Cui Y,Groth S,Troutman S,Carlstedt A,Sperka T,Riecken LB,Kissil JL,Jin H,Morrison H

Sun Sep 01 00:00:00 EDT 2019

Human Pluripotent Stem Cell-Derived Multipotent Vascular Progenitors of the Mesothelium Lineage Have Utility in Tissue Engineering and Repair.

Cell reports

Colunga T,Hayworth M,Kreß S,Reynolds DM,Chen L,Nazor KL,Baur J,Singh AM,Loring JF,Metzger M,Dalton S

AF-100-03 was used in Cell Culture to evaluate the use of human pluripotent stem cell-derived vascular progenitor cell in tissue engineering and repair in neontal mouse heart.

Tue Mar 05 00:00:00 EST 2019

Dystrophin deficiency leads to dysfunctional glutamate clearance in iPSC derived astrocytes.

Translational psychiatry

Patel AM,Wierda K,Thorrez L,van Putten M,De Smedt J,Ribeiro L,Tricot T,Gajjar M,Duelen R,Van Damme P,De Waele L,Goemans N,Tanganyika-de Winter C,Costamagna D,Aartsma-Rus A,van Duyvenvoorde H,Sampaolesi M,Buyse GM,Verfaillie CM

Wed Aug 21 00:00:00 EDT 2019

Neural Progenitor-Like Cells Induced from Human Gingiva-Derived Mesenchymal Stem Cells Regulate Myelination of Schwann Cells in Rat Sciatic Nerve Regeneration.

Stem cells translational medicine

Zhang Q,Nguyen P,Xu Q,Park W,Lee S,Furuhashi A,Le AD

Wed Feb 01 00:00:00 EST 2017

Proteomics and transcriptomics of peripheral nerve tissue and cells unravel new aspects of the human Schwann cell repair phenotype.

Glia

Weiss T,Taschner-Mandl S,Bileck A,Slany A,Kromp F,Rifatbegovic F,Frech C,Windhager R,Kitzinger H,Tzou CH,Ambros PF,Gerner C,Ambros IM

Thu Dec 01 00:00:00 EST 2016

Neuregulin mediates F-actin-driven cell migration through inhibition of protein kinase D1 via Rac1 protein.

The Journal of biological chemistry

Döppler H,Bastea LI,Eiseler T,Storz P

Fri Jan 04 00:00:00 EST 2013

A functional comparison between the HER2(high)/HER3 and the HER2(low)/HER3 dimers on heregulin-β1-induced MMP-1 and MMP-9 expression in breast cancer cells.

Experimental & molecular medicine

Kim S,Han J,Shin I,Kil WH,Lee JE,Nam SJ

Fri Aug 31 00:00:00 EDT 2012

2D protrusion but not motility predicts growth factor-induced cancer cell migration in 3D collagen.

The Journal of cell biology

Meyer AS,Hughes-Alford SK,Kay JE,Castillo A,Wells A,Gertler FB,Lauffenburger DA

Mon Jun 11 00:00:00 EDT 2012

Impaired Pten expression in human malignant peripheral nerve sheath tumours.

PloS one

Bradtmöller M,Hartmann C,Zietsch J,Jäschke S,Mautner VF,Kurtz A,Park SJ,Baier M,Harder A,Reuss D,von Deimling A,Heppner FL,Holtkamp N

Tue Apr 23 00:00:00 EDT 2013

Impaired Pten expression in human malignant peripheral nerve sheath tumours.

PloS one

Bradtmöller M,Hartmann C,Zietsch J,Jäschke S,Mautner VF,Kurtz A,Park SJ,Baier M,Harder A,Reuss D,von Deimling A,Heppner FL,Holtkamp N

Tue Apr 23 00:00:00 EDT 2013

A scalable system for production of functional pancreatic progenitors from human embryonic stem cells.

PloS one

Schulz TC,Young HY,Agulnick AD,Babin MJ,Baetge EE,Bang AG,Bhoumik A,Cepa I,Cesario RM,Haakmeester C,Kadoya K,Kelly JR,Kerr J,Martinson LA,McLean AB,Moorman MA,Payne JK,Richardson M,Ross KG,Sherrer ES,Song X,Wilson AZ,Brandon EP,Green CE,Kroon EJ,Kelly OG,D'Amour KA,Robins AJ

Wed Sep 19 00:00:00 EDT 2012

Novel mechanism for negatively regulating Rho-kinase (ROCK) signaling through Coronin1B protein in neuregulin 1 (NRG-1)-induced tumor cell motility.

The Journal of biological chemistry

Rana MK,Worthylake RA

Fri Jun 22 00:00:00 EDT 2012

The metabolic regulator ERRα, a downstream target of HER2/IGF-1R, as a therapeutic target in breast cancer.

Cancer cell

Chang CY,Kazmin D,Jasper JS,Kunder R,Zuercher WJ,McDonnell DP

Tue Oct 18 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: acetylcholine receptor-inducing activity; glial growth factor 2; heregulin, alpha (45kD, ERBB2 p185-activator); neu differentiation factor; Pro-neuregulin-1, membrane-bound isoform; Pro-NRG1; sensory and motor neuron derived factor

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Gene Aliases: ARIA; GGF; GGF2; HGL; HRG; HRG1; HRGA; MST131; MSTP131; NDF; NRG1; NRG1-IT2; SMDF

Human Heregulin beta-1, Animal-Free Recombinant Protein, PeproTech®

Human Heregulin beta-1, Animal-Free Protein (AF-100-03-50UG) in Functional

Product Details

AF-100-03-50UG

Functional Assay (Functional)

In vitro Assay (IV)

Miscellaneous PubMed (Misc)

Species

Published species

Expression System

Amino acid sequence

Molecular weight

Class

Type

Purity

Endotoxin concentration

Activity

Conjugate

Form

Purification

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

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