Gibco

Human I-309 (CCL1) Recombinant Protein, PeproTech®

Human I-309 (CCL1) Protein in Functional Assay (Functional)

FIGURE: 1 / 1

Human I-309 (CCL1) Protein (300-37-10UG) in Functional

Bioassay analysis of Human I-309 (CCL1) Recombinant Protein, PeproTech® (Product # 300-37-1MG).

Product Image Gallery

Product Details

300-37-10UG

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

ELISA (ELISA)

Assay-dependent

View 2 publications 2 publications

Functional Assay (Functional)

Assay-dependent

In vitro Assay (IV)

View 20 publications 20 publications

Miscellaneous PubMed (Misc)

View 3 publications 3 publications

Product Specifications

Species

Human

Published species

Bacteria, Human, Mouse, Non-human primate

Expression System

E. coli

Amino acid sequence

SKSMQVPFSR CCFSFAEQEI PLRAILCYRN TSSICSNEGL IFKLKRGKEA CALDTVGWVQ RHRKMLRHCP SKRK

Molecular weight

8.5 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

Determined by its ability to chemoattract human monocytes population using a concentration range of 10.0-30.0 ng/ml.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Human I-309 is an 8.5 kDa protein consisting of 74 amino acid residues that include six cysteine residues, of which four are the highly conserved cysteine residues present in CC chemokines. Unlike some CC chemokines, whose biologically active form in solution is a non-disulfide-linked dimer, the biologically active form of I-309 is monomeric.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine is secreted by activated T cells and displays chemotactic activity for monocytes but not for neutrophils. It binds to the chemokine receptor CCR8.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Increased both PD-L1 and PD-L2 expressions on monocytes of patients with hepatocellular carcinoma was associated with a poor prognosis.

Scientific reports

Yasuoka H,Asai A,Ohama H,Tsuchimoto Y,Fukunishi S,Higuchi K

Thu Jun 25 00:00:00 EDT 2020

Cytotoxic T cells swarm by homotypic chemokine signalling.

eLife

Galeano Niño JL,Pageon SV,Tay SS,Colakoglu F,Kempe D,Hywood J,Mazalo JK,Cremasco J,Govendir MA,Dagley LF,Hsu K,Rizzetto S,Zieba J,Rice G,Prior V,O'Neill GM,Williams RJ,Nisbet DR,Kramer B,Webb AI,Luciani F,Read MN,Biro M

Tue Oct 13 00:00:00 EDT 2020

A knottin scaffold directs the CXC-chemokine-binding specificity of tick evasins.

The Journal of biological chemistry

Lee AW,Deruaz M,Lynch C,Davies G,Singh K,Alenazi Y,Eaton JRO,Kawamura A,Shaw J,Proudfoot AEI,Dias JM,Bhattacharya S

Fri Jul 19 00:00:00 EDT 2019

Genetically engineered two-warhead evasins provide a method to achieve precision targeting of disease-relevant chemokine subsets.

Scientific reports

Alenazi Y,Singh K,Davies G,Eaton JRO,Elders P,Kawamura A,Bhattacharya S

Fri Apr 20 00:00:00 EDT 2018

Role of Conserved Disulfide Bridges and Aromatic Residues in Extracellular Loop 2 of Chemokine Receptor CCR8 for Chemokine and Small Molecule Binding.

The Journal of biological chemistry

Barington L,Rummel PC,Lückmann M,Pihl H,Larsen O,Daugvilaite V,Johnsen AH,Frimurer TM,Karlshøj S,Rosenkilde MM

Fri Jul 29 00:00:00 EDT 2016

M2b Monocytes Provoke Bacterial Pneumonia and Gut Bacteria-Associated Sepsis in Alcoholics.

Journal of immunology (Baltimore, Md. : 1950)

Tsuchimoto Y,Asai A,Tsuda Y,Ito I,Nishiguchi T,Garcia MC,Suzuki S,Kobayashi M,Higuchi K,Suzuki F

Tue Dec 01 00:00:00 EST 2015

Host chemokines bind to Staphylococcus aureus and stimulate protein A release.

The Journal of biological chemistry

Yung SC,Parenti D,Murphy PM

Fri Feb 18 00:00:00 EST 2011

M2b monocytes predominated in peripheral blood of severely burned patients.

Journal of immunology (Baltimore, Md. : 1950)

Kobayashi M,Jeschke MG,Shigematsu K,Asai A,Yoshida S,Herndon DN,Suzuki F

Wed Dec 15 00:00:00 EST 2010

Structure and function of A41, a vaccinia virus chemokine binding protein.

PLoS pathogens

Bahar MW,Kenyon JC,Putz MM,Abrescia NG,Pease JE,Wise EL,Stuart DI,Smith GL,Grimes JM

Tue Jan 01 00:00:00 EST 2008

Statins induce regulatory T cell recruitment via a CCL1 dependent pathway.

Journal of immunology (Baltimore, Md. : 1950)

Mira E,León B,Barber DF,Jiménez-Baranda S,Goya I,Almonacid L,Márquez G,Zaballos A,Martínez-A C,Stein JV,Ardavín C,Mañes S

Mon Sep 01 00:00:00 EDT 2008

XCL1 enhances regulatory activities of CD4+ CD25(high) CD127(low/-) T cells in human allergic asthma.

Journal of immunology (Baltimore, Md. : 1950)

Nguyen KD,Fohner A,Booker JD,Dong C,Krensky AM,Nadeau KC

Wed Oct 15 00:00:00 EDT 2008

CC-chemokine ligand 16 induces a novel maturation program in human immature monocyte-derived dendritic cells.

Journal of immunology (Baltimore, Md. : 1950)

Cappello P,Fraone T,Barberis L,Costa C,Hirsch E,Elia AR,Caorsi C,Musso T,Novelli F,Giovarelli M

Wed Nov 01 00:00:00 EST 2006

Autoantigens signal through chemokine receptors: uveitis antigens induce CXCR3- and CXCR5-expressing lymphocytes and immature dendritic cells to migrate.

Blood

Howard OM,Dong HF,Su SB,Caspi RR,Chen X,Plotz P,Oppenheim JJ

Wed Jun 01 00:00:00 EDT 2005

Human herpesvirus 7 open reading frames U12 and U51 encode functional beta-chemokine receptors.

Journal of virology

Tadagaki K,Nakano K,Yamanishi K

Wed Jun 01 00:00:00 EDT 2005

CCR4 ligands are up-regulated in the airways of atopic asthmatics after segmental allergen challenge.

The European respiratory journal

Pilette C,Francis JN,Till SJ,Durham SR

Tue Jun 01 00:00:00 EDT 2004

CCR4 ligands are up-regulated in the airways of atopic asthmatics after segmental allergen challenge.

The European respiratory journal

Pilette C,Francis JN,Till SJ,Durham SR

Tue Jun 01 00:00:00 EDT 2004

Viral macrophage-inflammatory protein-II: a viral chemokine that differentially affects adaptive mucosal immunity compared with its mammalian counterparts.

Journal of immunology (Baltimore, Md. : 1950)

Singh UP,Singh S,Ravichandran P,Taub DD,Lillard JW

Mon Nov 01 00:00:00 EST 2004

Human P-selectin glycoprotein ligand-1 (PSGL-1) interacts with the skin-associated chemokine CCL27 via sulfated tyrosines at the PSGL-1 amino terminus.

The Journal of biological chemistry

Hirata T,Furukawa Y,Yang BG,Hieshima K,Fukuda M,Kannagi R,Yoshie O,Miyasaka M

Fri Dec 10 00:00:00 EST 2004

Marked increase in CC chemokine gene expression in both human and mouse mast cell transcriptomes following Fcepsilon receptor I cross-linking: an interspecies comparison.

Blood

Nakajima T,Inagaki N,Tanaka H,Tanaka A,Yoshikawa M,Tamari M,Hasegawa K,Matsumoto K,Tachimoto H,Ebisawa M,Tsujimoto G,Matsuda H,Nagai H,Saito H

Sun Dec 01 00:00:00 EST 2002

Hematopoietic stem cells are uniquely selective in their migratory response to chemokines.

The Journal of experimental medicine

Wright DE,Bowman EP,Wagers AJ,Butcher EC,Weissman IL

Mon May 06 00:00:00 EDT 2002

Amyloid-beta induces chemotaxis and oxidant stress by acting at formylpeptide receptor 2, a G protein-coupled receptor expressed in phagocytes and brain.

The Journal of biological chemistry

Tiffany HL,Lavigne MC,Cui YH,Wang JM,Leto TL,Gao JL,Murphy PM

Fri Jun 29 00:00:00 EDT 2001

Developmental switches in chemokine response profiles during B cell differentiation and maturation.

The Journal of experimental medicine

Bowman EP,Campbell JJ,Soler D,Dong Z,Manlongat N,Picarella D,Hardy RR,Butcher EC

Mon Apr 17 00:00:00 EDT 2000

Chemokines and the arrest of lymphocytes rolling under flow conditions.

Science (New York, N.Y.)

Campbell JJ,Hedrick J,Zlotnik A,Siani MA,Thompson DA,Butcher EC

Fri Jan 16 00:00:00 EST 1998

Identification of single C motif-1/lymphotactin receptor XCR1.

The Journal of biological chemistry

Yoshida T,Imai T,Kakizaki M,Nishimura M,Takagi S,Yoshie O

Fri Jun 26 00:00:00 EDT 1998

Cloning and characterization of a specific receptor for the novel CC chemokine MIP-3alpha from lung dendritic cells.

The Journal of experimental medicine

Power CA,Church DJ,Meyer A,Alouani S,Proudfoot AE,Clark-Lewis I,Sozzani S,Mantovani A,Wells TN

Mon Sep 15 00:00:00 EDT 1997

Bioinformatics

Protein Aliases: C-C motif chemokine 1; CCL 1; chemokine (C-C motif) ligand 1; inflammatory cytokine I-309; small inducible cytokine A1 (I-309, homologous to mouse Tca-3); Small-inducible cytokine A1; T lymphocyte-secreted protein I-309

Human I-309 (CCL1) Recombinant Protein, PeproTech®

Human I-309 (CCL1) Protein (300-37-10UG) in Functional

Product Details

300-37-10UG

Western Blot (WB)

ELISA (ELISA)

Functional Assay (Functional)

In vitro Assay (IV)

Miscellaneous PubMed (Misc)

Species

Published species

Expression System

Amino acid sequence

Molecular weight

Class

Type

Purity

Endotoxin concentration

Activity

Conjugate

Form

Purification

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

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