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Human IGF-I, Animal-Free Recombinant Protein, PeproTech®

Human IGF-I, Animal-Free Protein in Functional Assay (Functional)

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Human IGF-I, Animal-Free Protein (AF-100-11-500UG) in Functional

WHO Comparison of Human IGF-I, Animal-Free Recombinant Protein, PeproTech® (Product # AF-100-11-1MG).

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Product Details

AF-100-11-500UG

Applications

Tested Dilution

Publications

Functional Assay (Functional)

Assay-dependent

In vitro Assay (IV)

View 28 publications 28 publications

Miscellaneous PubMed (Misc)

View 7 publications 7 publications

Product Specifications

Species

Human

Published species

Human, Mouse, Non-human primate, Rat

Expression System

E. coli

Amino acid sequence

GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA

Molecular weight

7.6 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<0.1 EU/µg

Activity

The ED50 was determined by a cell proliferation assay using FDC-P1 cells is ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 5 x 10^5 units/mg.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Human IGF-I and IGF-II are globular proteins containing 70 and 67 amino acids, respectively, and 3 intra-molecular disulfide bonds. The calculated molecular weight of Recombinant Human IGF-I is 7.6 kDa.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

IGF1 (Insulin-like growth factor-1) is structurally and functionally related to insulin but has a much higher growth-promoting activity. A variety of cellular responses are induced by IGF1, including cell proliferation, differentiation, migration, and survival. Further, IGF1 is a polypeptide growth factor that stimulates the proliferation of a wide range of cell types in muscle, bone, and cartilage tissue. IGF1 stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regards to enhancing glucose uptake. In circulation, IGFs are predominantly bound to binding proteins (IGFBPs) which prolong the half-life of the IGFs and play a role in delivering them to target tissues. IGF-I is known as one of the most potent activators of the AKT signaling pathway which is known to be a stimulator of proliferation and an inhibitor of programmed cell death. Moreover, mature human IGF-I is 100% homologous with bovine and porcine proteins. Low levels of IGF1 have been linked to Alzheimer's disease. IGF1 is processed from a precursor, bound by a specific receptor, and secreted. Defects in the IGF1 gene are a cause of insulin-like growth factor 1 deficiency and several transcript variants encoding different isoforms have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

iPSC-derived myelinoids to study myelin biology of humans.

Developmental cell

James OG,Selvaraj BT,Magnani D,Burr K,Connick P,Barton SK,Vasistha NA,Hampton DW,Story D,Smigiel R,Ploski R,Brophy PJ,Ffrench-Constant C,Lyons DA,Chandran S

Mon May 03 00:00:00 EDT 2021

Hyperglycaemia up-regulates placental growth factor (PlGF) expression and secretion in endothelial cells via suppression of PI3 kinase-Akt signalling and activation of FOXO1.

Scientific reports

Sissaoui S,Egginton S,Ting L,Ahmed A,Hewett PW

Wed Aug 11 00:00:00 EDT 2021

Inhibition of aquaporin-1 prevents myocardial remodeling by blocking the transmembrane transport of hydrogen peroxide.

Science translational medicine

Montiel V,Bella R,Michel LYM,Esfahani H,De Mulder D,Robinson EL,Deglasse JP,Tiburcy M,Chow PH,Jonas JC,Gilon P,Steinhorn B,Michel T,Beauloye C,Bertrand L,Farah C,Dei Zotti F,Debaix H,Bouzin C,Brusa D,Horman S,Vanoverschelde JL,Bergmann O,Gilis D,Rooman M,Ghigo A,Geninatti-Crich S,Yool A,Zimmermann WH,Roderick HL,Devuyst O,Balligand JL

AF-100-11 was used in Cell Culture to investigate regulators of oxidant-mediated cardiac hypertrophy.

Wed Oct 07 00:00:00 EDT 2020

3D Bioprinting Pluripotent Stem Cell Derived Neural Tissues Using a Novel Fibrin Bioink Containing Drug Releasing Microspheres.

Frontiers in bioengineering and biotechnology

Sharma R,Smits IPM,De La Vega L,Lee C,Willerth SM

Mon Nov 13 00:00:00 EST 2023

Generation of oligodendrocytes and establishment of an all-human myelinating platform from human pluripotent stem cells.

Nature protocols

García-León JA,García-Díaz B,Eggermont K,Cáceres-Palomo L,Neyrinck K,Madeiro da Costa R,Dávila JC,Baron-Van Evercooren A,Gutiérrez A,Verfaillie CM

Sun Nov 01 00:00:00 EDT 2020

Novel Analytical Platform For Robust Identification of Cell Migration Inhibitors.

Scientific reports

Somchai P,Phongkitkarun K,Kueanjinda P,Jamnongsong S,Vaeteewoottacharn K,Luvira V,Okada S,Jirawatnotai S,Sampattavanich S

AF-100-11 was used in In vitro experiments to test cell migration inhibitors.

Wed Jan 22 00:00:00 EST 2020

Generation of Engineered Human Myocardium in a Multi-well Format.

STAR protocols

Tiburcy M,Meyer T,Liaw NY,Zimmermann WH

Fri Jun 19 00:00:00 EDT 2020

The arginine methyltransferase PRMT1 regulates IGF-1 signaling in breast cancer.

Oncogene

Choucair A,Pham TH,Omarjee S,Jacquemetton J,Kassem L,Trédan O,Rambaud J,Marangoni E,Corbo L,Treilleux I,Le Romancer M

Wed May 01 00:00:00 EDT 2019

Single-cell qPCR demonstrates that Repsox treatment changes cell fate from endoderm to neuroectoderm and disrupts epithelial-mesenchymal transition.

PloS one

Li Q,Huang Q

AF-100-11 was used in Cell Culture to show that the process of epithelial-to-mesenchymal transition, which is inherent to the process of definitive endoderm differentiation, was also disrupted upon Repsox treatment. Our findings may provide a new approach to produce neural progenitors.

Wed Mar 11 00:00:00 EDT 2020

Efficacy of Two Delivery Routes for Transplanting Human Neural Progenitor Cells (NPCs) Into the Spastic Han-Wistar Rat, a Model of Ataxia.

Cell transplantation

Uhlendorf TL,Nuryyev RL,Kopyov AO,Ochoa J,Younesi S,Cohen RW,Kopyov OV

AF-100-11 was used in Cell Culture to show that direct implantation of hNPCs reduced ataxic symptoms in the sHW rat, demonstrating that stereotactic route of stem cell delivery correlates to improved clinical outcomes.

Thu Feb 16 00:00:00 EST 2017

Acute resistance exercise-induced IGF1 expression and subsequent GLUT4 translocation.

Physiological reports

Kido K,Ato S,Yokokawa T,Makanae Y,Sato K,Fujita S

Mon Aug 01 00:00:00 EDT 2016

Subtype-specific regeneration of retinal ganglion cells following axotomy: effects of osteopontin and mTOR signaling.

Neuron

Duan X,Qiao M,Bei F,Kim IJ,He Z,Sanes JR

Wed Mar 18 00:00:00 EDT 2015

Cytohesins/ARNO: the function in colorectal cancer cells.

PloS one

Pan T,Sun J,Hu J,Hu Y,Zhou J,Chen Z,Xu D,Xu W,Zheng S,Zhang S

AF-100-11 was used in In vitro experiments to investigate the role of EGF and IGF-I in colorectal cancer tissue compared to adjacent benign tissue.

Fri May 29 00:00:00 EDT 2015

IGF-1 promotes the development and cytotoxic activity of human NK cells.

Nature communications

Ni F,Sun R,Fu B,Wang F,Guo C,Tian Z,Wei H

Thu Jun 20 00:00:00 EDT 2013

A brain-specific Grb2-associated regulator of extracellular signal-regulated kinase (Erk)/mitogen-activated protein kinase (MAPK) (GAREM) subtype, GAREM2, contributes to neurite outgrowth of neuroblastoma cells by regulating Erk signaling.

The Journal of biological chemistry

Taniguchi T,Tanaka S,Ishii A,Watanabe M,Fujitani N,Sugeo A,Gotoh S,Ohta T,Hiyoshi M,Matsuzaki H,Sakai N,Konishi H

Fri Oct 11 00:00:00 EDT 2013

Anthraquinone-2-sulfonic acid (AQ2S) is a novel neurotherapeutic agent.

Cell death & disease

Jackson TC,Verrier JD,Kochanek PM

Thu Jan 10 00:00:00 EST 2013

Human ES-cell-derived cardiomyocytes electrically couple and suppress arrhythmias in injured hearts.

Nature

Shiba Y,Fernandes S,Zhu WZ,Filice D,Muskheli V,Kim J,Palpant NJ,Gantz J,Moyes KW,Reinecke H,Van Biber B,Dardas T,Mignone JL,Izawa A,Hanna R,Viswanathan M,Gold JD,Kotlikoff MI,Sarvazyan N,Kay MW,Murry CE,Laflamme MA

Thu Sep 13 00:00:00 EDT 2012

Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.

Nature

Wilson TR,Fridlyand J,Yan Y,Penuel E,Burton L,Chan E,Peng J,Lin E,Wang Y,Sosman J,Ribas A,Li J,Moffat J,Sutherlin DP,Koeppen H,Merchant M,Neve R,Settleman J

Thu Jul 26 00:00:00 EDT 2012

2D protrusion but not motility predicts growth factor-induced cancer cell migration in 3D collagen.

The Journal of cell biology

Meyer AS,Hughes-Alford SK,Kay JE,Castillo A,Wells A,Gertler FB,Lauffenburger DA

Mon Jun 11 00:00:00 EDT 2012

Pharmacological modulation of human mesenchymal stem cell chondrogenesis by a chemically oversulfated polysaccharide of marine origin: potential application to cartilage regenerative medicine.

Stem cells (Dayton, Ohio)

Merceron C,Portron S,Vignes-Colombeix C,Rederstorff E,Masson M,Lesoeur J,Sourice S,Sinquin C,Colliec-Jouault S,Weiss P,Vinatier C,Guicheux J

Thu Mar 01 00:00:00 EST 2012

ROS constitute a convergence nexus in the development of IGF1 resistance and impaired wound healing in a rat model of type 2 diabetes.

Disease models & mechanisms

Bitar MS,Al-Mulla F

Tue May 01 00:00:00 EDT 2012

Growth factors released from gelatin hydrogel microspheres increase new neurons in the adult mouse brain.

Stem cells international

Nakaguchi K,Jinnou H,Kaneko N,Sawada M,Hikita T,Saitoh S,Tabata Y,Sawamoto K

Thu Oct 25 00:00:00 EDT 2012

Dental pulp stem cells differentiation reveals new insights in Oct4A dynamics.

PloS one

Ferro F,Spelat R,D'Aurizio F,Puppato E,Pandolfi M,Beltrami AP,Cesselli D,Falini G,Beltrami CA,Curcio F

Wed Nov 28 00:00:00 EST 2012

Insulin-like growth factor-I receptor (IGF-IR) translocates to nucleus and autoregulates IGF-IR gene expression in breast cancer cells.

The Journal of biological chemistry

Sarfstein R,Pasmanik-Chor M,Yeheskel A,Edry L,Shomron N,Warman N,Wertheimer E,Maor S,Shochat L,Werner H

Fri Jan 20 00:00:00 EST 2012

Promotion of human early embryonic development and blastocyst outgrowth in vitro using autocrine/paracrine growth factors.

PloS one

Kawamura K,Chen Y,Shu Y,Cheng Y,Qiao J,Behr B,Pera RA,Hsueh AJ

Tue May 07 00:00:00 EDT 2013

Sphingosine kinase/sphingosine 1-phosphate axis: a new player for insulin-like growth factor-1-induced myoblast differentiation.

Skeletal muscle

Bernacchioni C,Cencetti F,Blescia S,Donati C,Bruni P

Thu Jul 12 00:00:00 EDT 2012

Phase contrast microscopy of living cells within the whole lens: spatial correlations and morphological dynamics.

Molecular vision

Kong Z,Zhu X,Zhang S,Wu J,Luo Y

Fri Dec 21 00:00:00 EST 2012

Anti-proliferative effect of cytohesin inhibition in gefitinib-resistant lung cancer cells.

PloS one

Bill A,Schmitz A,König K,Heukamp LC,Hannam JS,Famulok M

Wed Jan 16 00:00:00 EST 2013

A Novel Role of IGF1 in Apo2L/TRAIL-Mediated Apoptosis of Ewing Tumor Cells.

Sarcoma

van Valen F,Harrer H,Hotfilder M,Dirksen U,Pap T,Gosheger G,Humpf HU,Jürgens H

Wed Oct 24 00:00:00 EDT 2012

The metabolic regulator ERRα, a downstream target of HER2/IGF-1R, as a therapeutic target in breast cancer.

Cancer cell

Chang CY,Kazmin D,Jasper JS,Kunder R,Zuercher WJ,McDonnell DP

Tue Oct 18 00:00:00 EDT 2011

High-level IGF1R expression is required for leukemia-initiating cell activity in T-ALL and is supported by Notch signaling.

The Journal of experimental medicine

Medyouf H,Gusscott S,Wang H,Tseng JC,Wai C,Nemirovsky O,Trumpp A,Pflumio F,Carboni J,Gottardis M,Pollak M,Kung AL,Aster JC,Holzenberger M,Weng AP

Mon Aug 29 00:00:00 EDT 2011

Phosphatase and tensin homolog regulates the pluripotent state and lineage fate choice in human embryonic stem cells.

Stem cells (Dayton, Ohio)

Alva JA,Lee GE,Escobar EE,Pyle AD

Thu Dec 01 00:00:00 EST 2011

Rac1 and Stathmin but Not EB1 Are Required for Invasion of Breast Cancer Cells in Response to IGF-I.

International journal of cell biology

Morimura S,Takahashi K

Thu Nov 10 00:00:00 EST 2011

The mechanism of action of the histone deacetylase inhibitor vorinostat involves interaction with the insulin-like growth factor signaling pathway.

PloS one

Sarfstein R,Bruchim I,Fishman A,Werner H

Fri Jun 08 00:00:00 EDT 2012

The mTOR (mammalian target of rapamycin) kinase maintains integrity of mTOR complex 2.

The Journal of biological chemistry

Chen CH,Sarbassov dos D

Fri Nov 18 00:00:00 EST 2011

Bioinformatics

Protein Aliases: H-IGF-1; IGF; IGF-I; IGF-IA; IGF-IB; IGF1A; IGFIa; Insulin like growth factor; insulin-like growth factor 1 (somatomedin C); Insulin-like growth factor I; insulin-like growth factor IB; M-IGF-1; Mechano growth factor; MGF; OTTHUMP00000195084; R-IGF-1; Somatomedin C; Somatomedin-C

Human IGF-I, Animal-Free Recombinant Protein, PeproTech®

Human IGF-I, Animal-Free Protein (AF-100-11-500UG) in Functional

Product Details

AF-100-11-500UG

Functional Assay (Functional)

In vitro Assay (IV)

Miscellaneous PubMed (Misc)

Species

Published species

Expression System

Amino acid sequence

Molecular weight

Class

Type

Purity

Endotoxin concentration

Activity

Conjugate

Form

Purification

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

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