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Invitrogen
Human IL-10 (ELISA Standard) Recombinant Protein, eBioscience™
Product Details
29-8109-65
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
Human IL-10, amino acids Ser19-Asn178 (Accession # NM_000572)
Molecular weight
18 kDa
Class
Recombinant
Type
Protein
Conjugate
Form
Lyophilized
Purification
purified
Contains
no preservative
Storage conditions
4° C
Shipping conditions
Wet ice
Product Specific Information
Description: Human IL-10 is a ~18 kDa factor also known as cytokine synthesis inhibitory factor (CSIF). Th2 T cells, B1 B cells and keratinocytes are the major cell subsets that produce IL-10. IL-10 inhibits synthesis of Th1 cytokines and proliferation of T cells and acts as a costimulatory signal for mast cells, developing thymocytes and Th2 response.
Applications Reported: Human IL-10 RSG ELISA Lyophilized Standard has been reported for use in the Human IL-10 ELISA RSG (Product # 88-7106).
Applications Tested: The intended use of the Human IL-10 RSG ELISA Lyophilized Standard is as a standard for the Human IL-10 ELISA (Product # 88-7106-77). Typically the standard is run in a series of 7 doubling dilutions from the 'top' standard. Please refer to the Certificate of Analysis included with each ELISA set for instructions on the proper reconstitution and dilution of the lyophilized standard.
Note: These are single-use vials. Use one time within 4 hours of reconstitution and discard. Reconstitute and dilute as suggested in the Certificate of Analysis or recovery may not be accurate.
Source: E. coli expressed amino acids Ser 19-Asn 178 of mature human IL-10 accession # NM_000572.
Target Information
IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CSIF; Cytokine synthesis inhibitory factor; H-IL-10; il 10; IL-10; ILN; Interleukin; Interleukin-10; Interleukin10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor
Gene Aliases: CSIF; GVHDS; IL-10; IL10; IL10A; TGIF
UniProt ID: (Human) P22301
Entrez Gene ID: (Human) 3586
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