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Product Details
PHC0101
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Molecular weight
18.6 kDa
Class
Recombinant
Type
Protein
Purity
>95% by SDS-PAGE
Endotoxin concentration
<0.1 ng/µg
Activity
ED50 = 0.2 - 1.0 ng/mL; determined by the dose-dependent proliferation of murine MC/9 cells.
Conjugate
Form
Lyophilized
Purification
purified
Contains
no preservative
Storage conditions
4° C
Shipping conditions
Wet ice (domestic); Wet or Dry ice (international)
Product Specific Information
Carrier-Free
Reconstitution: Centrifuge the vial briefly, before opening to bring the contents to the bottom. Reconstitute the lyophilized protein in sterile water or other appropriate buffered solutions to 0.1-1.0 mg/mL. Apportion the reconstituted protein into working aliquots and store at ≤-20°C. Make any further dilutions of the reconstituted protein in solution with carrier protein, such as PBS with 1% BSA.
Storage: Store the lyophilized protein at 2-8°C or -20°C long term, preferably desiccated. Upon reconstitution, apportion into working aliquots and store at ≤-20°C. Avoid repeated freeze-thaw cycles.
Target Information
IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CSIF; Cytokine synthesis inhibitory factor; H-IL-10; il 10; IL-10; ILN; Interleukin; Interleukin-10; Interleukin10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor
Gene Aliases: CSIF; GVHDS; IL-10; IL10; IL10A; TGIF
UniProt ID: (Human) P22301
Entrez Gene ID: (Human) 3586
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