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Product Details
RIL1025
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
Human IL-10 recombinant protein contains 161 amino acids
Molecular weight
18.6 kDa
Class
Recombinant
Type
Protein
Purity
>98% by SDS-PAGE and FPLC
Activity
ED50 ≤ 2 ng/mL; determined by the dose-dependent proliferation of human IL-4 co-stimulated MC/9 cells.
Conjugate
Form
Lyophilized
Purification
purified
Storage buffer
PBS, pH 7.2
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Use: IL-10 exerts activity in the range of 0.2 to 20 ng/mL for most in vitro applications.
Reconstitution and Dilution: Reconstitute in distilled water to a concentration of 100 µg/mL. After reconstitution, dilute cytokine in a neutral buffer containing carrier protein such as 5-10% FBS or 1-4% high purity BSA (fatty acid free fraction V).
Lyophilized product is stable until expiration if stored desiccated below 0ºC. Reconstituted undiluted cytokine should be stored in working aliquots at -70ºC. Cytokines diluted as indicated above should be divided into aliquots and stored at -70ºC. Recombinant proteins should be stored at concentrations >500 ng/mL to reduce loss of activity.
Target Information
IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CSIF; Cytokine synthesis inhibitory factor; H-IL-10; il 10; IL-10; ILN; Interleukin; Interleukin-10; Interleukin10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor
Gene Aliases: CSIF; GVHDS; IL-10; IL10; IL10A; TGIF
UniProt ID: (Human) P22301
Entrez Gene ID: (Human) 3586
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