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Human IL-12 p70 Recombinant Protein, PeproTech®

Human IL-12 p70 Protein in Functional Assay (Functional)

FIGURE: 1 / 1

Human IL-12 p70 Protein (200-12H-10UG) in Functional

Bioassay analysis of Human IL-12 p70 Recombinant Protein, PeproTech® (Product # 200-12H-1MG).

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Product Details

200-12H-10UG

Applications

Tested Dilution

Publications

Functional Assay (Functional)

Assay-dependent

In vitro Assay (IV)

View 3 publications 3 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species

Human

Published species

Human

Expression System

HEK293 cells

Amino acid sequence

p35 subunit: RNLPVATPDP GMFPCLHHSQ NLLRAVSNML QKARQTLEFY PCTSEEIDHE DITKDKTSTV EACLPLELTK NESCLNSRET SFITNGSCLA SRKTSFMMAL CLSSIYEDLK MYQVEFKTMN AKLLMDPKRQ IFLDQNMLAV IDELMQALNF NSETVPQKSS LEEPDFYKTK IKLCILLHAF RIRAVTIDRV MSYLNAS p40 subunit: IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK NASISVRAQD RYYSSSWSEW ASVPCS

Molecular weight

75 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

Determined by its ability to increase IFN-gamma production by anti-TCR mAb-stimulated PBMCs.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

PeproTech's Recombinant Human IL-12 p70, derived from HEK293 cells, is a heterodimeric glycoprotein consisting of disulfide-linked p35 and p40 subunits (503 total amino acid residues). The calculated molecular weight of Human IL-12 p70 is 57.2 kDa; however, due to glycosylation, it migrates at an apparent molecular weight of 65-70 kDa based on SDS-PAGE gel, under non-reducing conditions.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

IL-12 p70, also known as interleukin-12, is a cytokine that plays a critical role in the immune system's response to infections and cancer. It is produced by immune cells, including dendritic cells, macrophages, and B cells, in response to certain pathogens and tumor cells. IL-12 p70 stimulates the production of interferon-gamma and other cytokines, which promote the activation and proliferation of T cells and natural killer cells. IL-12 p70 has been studied as a potential therapeutic target for various diseases and conditions, including infectious diseases, autoimmune disorders, and cancer. It has been shown to enhance immune responses against certain infections and tumors, and to modulate inflammatory responses in autoimmune diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Untimely TGFβ responses in COVID-19 limit antiviral functions of NK cells.

Nature

Witkowski M,Tizian C,Ferreira-Gomes M,Niemeyer D,Jones TC,Heinrich F,Frischbutter S,Angermair S,Hohnstein T,Mattiola I,Nawrath P,McEwen S,Zocche S,Viviano E,Heinz GA,Maurer M,Kölsch U,Chua RL,Aschman T,Meisel C,Radke J,Sawitzki B,Roehmel J,Allers K,Moos V,Schneider T,Hanitsch L,Mall MA,Conrad C,Radbruch H,Duerr CU,Trapani JA,Marcenaro E,Kallinich T,Corman VM,Kurth F,Sander LE,Drosten C,Treskatsch S,Durek P,Kruglov A,Radbruch A,Mashreghi MF,Diefenbach A

200-12H was used in Cell Culture to investigate if a decline in viral load in COVID-19 correlates with Natural killer (NK) cell status and if NK cells can control SARS-CoV-2 replication by recognizing infected target cells.

Wed Dec 01 00:00:00 EST 2021

Ribosome-Targeting Antibiotics Impair T Cell Effector Function and Ameliorate Autoimmunity by Blocking Mitochondrial Protein Synthesis.

Immunity

Almeida L,Dhillon-LaBrooy A,Castro CN,Adossa N,Carriche GM,Guderian M,Lippens S,Dennerlein S,Hesse C,Lambrecht BN,Berod L,Schauser L,Blazar BR,Kalesse M,Müller R,Moita LF,Sparwasser T

Tue Jan 12 00:00:00 EST 2021

Selective deletion of human leukocyte antigens protects stem cell-derived islets from immune rejection.

Cell reports

Parent AV,Faleo G,Chavez J,Saxton M,Berrios DI,Kerper NR,Tang Q,Hebrok M

Tue Aug 17 00:00:00 EDT 2021

Single-cell RNA sequencing reveals the heterogeneity of liver-resident immune cells in human.

Cell discovery

Zhao J,Zhang S,Liu Y,He X,Qu M,Xu G,Wang H,Huang M,Pan J,Liu Z,Li Z,Liu L,Zhang Z

Tue Oct 03 00:00:00 EDT 2023

Bioinformatics

Protein Aliases: 20C2; CLMF p35; CLMF p40; cytotoxic lymphocyte maturation factor 1, p35; Cytotoxic lymphocyte maturation factor 35 kDa subunit; Cytotoxic lymphocyte maturation factor 40 kDa subunit; il 12; IL 12p70; IL-12 p75; IL-12 subunit p35; IL-12 subunit p40; IL-12, subunit p35; IL-12A; IL-12B; IL12, subunit p40; IL12A/B; IL12AB; IL35 subunit; ILN; Interleukin; interleukin 12, p35; interleukin 12, p40; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); interleukin-12 alpha chain; interleukin-12 beta chain; Interleukin-12 p70; Interleukin-12 subunit alpha; Interleukin-12 subunit beta; MGC151228; MGC151232; natural killer cell stimulatory factor, 40 kD subunit; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; NK cell stimulatory factor chain 2; NKSF1; NKSF2

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Gene Aliases: CLMF; CLMF2; IL-12A; IL-12B; IL12A; IL12B; IMD28; IMD29; NFSK; NKSF; NKSF1; NKSF2; P35