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Human IL-21, Animal-Free Recombinant Protein, PeproTech®

Human IL-21, Animal-Free Protein in Functional Assay (Functional)

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Human IL-21, Animal-Free Protein (AF-200-21-10UG) in Functional

Bioassay analysis of Human IL-21, Animal-Free Recombinant Protein, PeproTech® (Product # AF-200-21-1MG).

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Product Details

AF-200-21-10UG

Applications

Tested Dilution

Publications

Functional Assay (Functional)

Assay-dependent

In vitro Assay (IV)

View 18 publications 18 publications

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species

Human

Published species

Human, Mouse

Expression System

E. coli

Amino acid sequence

MQDRHMIRMR QLIDIVDQLK NYVNDLVPEF LPAPEDVETN CEWSAFSCFQ KAQLKSANTG NNERIINVSI KKLKRKPPST NAGRRQKHRL TCPSCDSYEK KPPKEFLERF KSLLQKMIHQ HLSSRTHGSE DS

Molecular weight

15.4 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<0.1 EU/µg

Activity

Determined by its ability to stimulate the proliferation of human ANBL-6 cells. The expected ED50 is ≤ 0.5 ng/ml, corresponding to a specific activity of ≥ 2 x10^6 units/mg.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Human IL-21 is a 15.4 kDa protein consisting of 132 amino acid residues.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

IL-21 is a 17 kDa immunomodulatory cytokine produced mainly by Natural Killer Cells (NKT), T helper (Th) 17 and T follicular helper (TFH) cells. In TFH cells, IL-21 expression leads to autocrine signaling through the IL-21 receptor (IL-21R) and STAT3, which leads to additional transcriptional activation by Bcl6. As with IFN gamma for Th1 and IL-4 for Th2 cells, IL-21 is critical for TFH cell development and effector function. IL-21 plays a role in T cell-dependent B cell differentiation into plasma cells and memory cells, stimulation of IgG production and induction of apoptotic signaling in naive B cells. In Th17 cells, IL-21 expression and autocrine feedback through STAT3, IRF4 and ROR gamma t lead to upregulation of the IL-23R, thereby preparing Th17 cells for maturation and maintenance by the inflammatory cytokine IL-23. While upregulating IRF4 and ROR gamma t, IL-21 also mediates the downregulation of Foxp3. IL-21 deficient mice are protected from developing colitis upon chemical treatment by their inability to upregulate Th17-associated molecules. In comparison, elevated levels of IL-21 are present in chemically-induced colitis mouse models.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Follicular regulatory T cells produce neuritin to regulate B cells.

Cell

Gonzalez-Figueroa P,Roco JA,Papa I,Núñez Villacís L,Stanley M,Linterman MA,Dent A,Canete PF,Vinuesa CG

Thu Apr 01 00:00:00 EDT 2021

Epigenetic modulation of immune synaptic-cytoskeletal networks potentiates γδ T cell-mediated cytotoxicity in lung cancer.

Nature communications

Weng RR,Lu HH,Lin CT,Fan CC,Lin RS,Huang TC,Lin SY,Huang YJ,Juan YH,Wu YC,Hung ZC,Liu C,Lin XH,Hsieh WC,Chiu TY,Liao JC,Chiu YL,Chen SY,Yu CJ,Tsai HC

Mon Apr 12 00:00:00 EDT 2021

A variant in IL6ST with a selective IL-11 signaling defect in human and mouse.

Bone research

Schwerd T,Krause F,Twigg SRF,Aschenbrenner D,Chen YH,Borgmeyer U,Müller M,Manrique S,Schumacher N,Wall SA,Jung J,Damm T,Glüer CC,Scheller J,Rose-John S,Jones EY,Laurence A,Wilkie AOM,Schmidt-Arras D,Uhlig HH

Mon Oct 02 00:00:00 EDT 2023

Janus kinase inhibitors for the treatment of rheumatoid arthritis demonstrate similar profiles of in vitro cytokine receptor inhibition.

Pharmacology research & perspectives

Dowty ME,Lin TH,Jesson MI,Hegen M,Martin DA,Katkade V,Menon S,Telliez JB

Sun Dec 01 00:00:00 EST 2019

RNA editing derived epitopes function as cancer antigens to elicit immune responses.

Nature communications

Zhang M,Fritsche J,Roszik J,Williams LJ,Peng X,Chiu Y,Tsou CC,Hoffgaard F,Goldfinger V,Schoor O,Talukder A,Forget MA,Haymaker C,Bernatchez C,Han L,Tsang YH,Kong K,Xu X,Scott KL,Singh-Jasuja H,Lizee G,Liang H,Weinschenk T,Mills GB,Hwu P

Tue Sep 25 00:00:00 EDT 2018

RNA editing derived epitopes function as cancer antigens to elicit immune responses.

Nature communications

Tue Sep 25 00:00:00 EDT 2018

Bioengineering T cells to target carbohydrate to treat opportunistic fungal infection.

Proceedings of the National Academy of Sciences of the United States of America

Kumaresan PR,Manuri PR,Albert ND,Maiti S,Singh H,Mi T,Roszik J,Rabinovich B,Olivares S,Krishnamurthy J,Zhang L,Najjar AM,Huls MH,Lee DA,Champlin RE,Kontoyiannis DP,Cooper LJ

Tue Jul 22 00:00:00 EDT 2014

IL-21 and CD40L synergistically promote plasma cell differentiation through upregulation of Blimp-1 in human B cells.

Journal of immunology (Baltimore, Md. : 1950)

Ding BB,Bi E,Chen H,Yu JJ,Ye BH

Fri Feb 15 00:00:00 EST 2013

Manufacture of clinical-grade CD19-specific T cells stably expressing chimeric antigen receptor using Sleeping Beauty system and artificial antigen presenting cells.

PloS one

Singh H,Figliola MJ,Dawson MJ,Olivares S,Zhang L,Yang G,Maiti S,Manuri P,Senyukov V,Jena B,Kebriaei P,Champlin RE,Huls H,Cooper LJ

AF-200-21 was used in Cell Culture to reveal how the platform technologies of SB-mediated transposition and CAR-dependent propagation on aAPC were adapted for human application.

Tue Feb 18 00:00:00 EST 2014

IRX-2, a novel immunotherapeutic, enhances functions of human dendritic cells.

PloS one

Schilling B,Harasymczuk M,Schuler P,Egan J,Ferrone S,Whiteside TL

Fri Sep 06 00:00:00 EDT 2013

Chimeric antigen receptor (CAR)-specific monoclonal antibody to detect CD19-specific T cells in clinical trials.

PloS one

Jena B,Maiti S,Huls H,Singh H,Lee DA,Champlin RE,Cooper LJ

Tue Aug 27 00:00:00 EDT 2013

IL-21 in the bone marrow microenvironment contributes to IgM secretion and proliferation of malignant cells in Waldenstrom macroglobulinemia.

Blood

Hodge LS,Ziesmer SC,Yang ZZ,Secreto FJ,Gertz MA,Novak AJ,Ansell SM

Thu Nov 01 00:00:00 EDT 2012

Cyclin-A1 represents a new immunogenic targetable antigen expressed in acute myeloid leukemia stem cells with characteristics of a cancer-testis antigen.

Blood

Ochsenreither S,Majeti R,Schmitt T,Stirewalt D,Keilholz U,Loeb KR,Wood B,Choi YE,Bleakley M,Warren EH,Hudecek M,Akatsuka Y,Weissman IL,Greenberg PD

Thu Jun 07 00:00:00 EDT 2012

Dependence on nuclear factor of activated T-cells (NFAT) levels discriminates conventional T cells from Foxp3+ regulatory T cells.

Proceedings of the National Academy of Sciences of the United States of America

Vaeth M,Schliesser U,Müller G,Reissig S,Satoh K,Tuettenberg A,Jonuleit H,Waisman A,Müller MR,Serfling E,Sawitzki BS,Berberich-Siebelt F

Tue Oct 02 00:00:00 EDT 2012

Soluble factors produced by activated CD4+ T cells modulate EBV latency.

Proceedings of the National Academy of Sciences of the United States of America

Nagy N,Adori M,Rasul A,Heuts F,Salamon D,Ujvári D,Madapura HS,Leveau B,Klein G,Klein E

Tue Jan 31 00:00:00 EST 2012

Cutting edge: IL-21 derived from human follicular helper T cells acts as a survival factor for secondary lymphoid organ, but not for bone marrow, plasma cells.

Journal of immunology (Baltimore, Md. : 1950)

Rodríguez-Bayona B,Ramos-Amaya A,Bernal J,Campos-Caro A,Brieva JA

Wed Feb 15 00:00:00 EST 2012

In vitro generation of long-lived human plasma cells.

Journal of immunology (Baltimore, Md. : 1950)

Cocco M,Stephenson S,Care MA,Newton D,Barnes NA,Davison A,Rawstron A,Westhead DR,Doody GM,Tooze RM

Sat Dec 15 00:00:00 EST 2012

Ex vivo expansion of human CD8+ T cells using autologous CD4+ T cell help.

PloS one

Butler MO,Imataki O,Yamashita Y,Tanaka M,Ansén S,Berezovskaya A,Metzler G,Milstein MI,Mooney MM,Murray AP,Mano H,Nadler LM,Hirano N

Mon Jul 09 00:00:00 EDT 2012

IL-21 is the primary common γ chain-binding cytokine required for human B-cell differentiation in vivo.

Blood

Recher M,Berglund LJ,Avery DT,Cowan MJ,Gennery AR,Smart J,Peake J,Wong M,Pai SY,Baxi S,Walter JE,Palendira U,Tangye GA,Rice M,Brothers S,Al-Herz W,Oettgen H,Eibel H,Puck JM,Cattaneo F,Ziegler JB,Giliani S,Tangye SG,Notarangelo LD

Thu Dec 22 00:00:00 EST 2011

IL-7 promotes CD95-induced apoptosis in B cells via the IFN-γ/STAT1 pathway.

PloS one

Sammicheli S,Dang VP,Ruffin N,Pham HT,Lantto R,Vivar N,Chiodi F,Rethi B

Mon Aug 27 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: CX2; il 21; IL-21; ILN; Interleukin; Interleukin-21; interleukin-21 isoform; Interleukin21; Za11

Human IL-21, Animal-Free Recombinant Protein, PeproTech®

Human IL-21, Animal-Free Protein (AF-200-21-10UG) in Functional

Product Details

AF-200-21-10UG

Functional Assay (Functional)

In vitro Assay (IV)

Miscellaneous PubMed (Misc)

Species

Published species

Expression System

Amino acid sequence

Molecular weight

Class

Type

Purity

Endotoxin concentration

Activity

Conjugate

Form

Purification

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

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