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Human IL-22 Recombinant Protein, PeproTech®

Human IL-22 Protein in Functional Assay (Functional)

FIGURE: 1 / 1

Human IL-22 Protein (200-22-10UG) in Functional

Bioassay analysis of Human IL-22 Recombinant Protein, PeproTech® (Product # 200-22-1MG).

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Product Details

200-22-10UG

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

View 1 publication 1 publication

Functional Assay (Functional)

Assay-dependent

In vitro Assay (IV)

View 26 publications 26 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species

Human

Published species

Human, Mouse, Non-human primate

Expression System

E. coli

Amino acid sequence

MAPISSHCRL DKSNFQQPYI TNRTFMLAKE ASLADNNTDV RLIGEKLFHG VSMSERCYLM KQVLNFTLEE VLFPQSDRFQ PYMQEVVPFL ARLSNRLSTC HIEGDDLHIQ RNVQKLKDTV KKLGESGEIK AIGELDLLFM SLRNACI

Molecular weight

33.6 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

Assay 1: Determined by its ability to activate STAT following receptor ligand interaction. Assay 2: Measured by its ability to induce IL-10 secretion in COLO 205 (human colon carcinoma cells). The expected ED50 for this effect is 0.7- 1.0 ng/ml.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Human IL-22 is a 33.6 kDa, non-disulfide-linked, homodimeric protein consisting of two 147 amino acid polypeptide chains.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

IL-22 also known as IL-10-related T-cell derived inducible factor, is an alpha helical cytokine and is considered a member of the IFN-IL-10 family, which includes IL-19, IL-20, IL-24, IL-26, IL-28, IL-29, and the type I and II interferons. IL-22 is produced mainly by activated T cells and NK cells. In humans, the IL-22 gene is located on the q arm of chromosome 12, and is structurally related to IL10. IL-22 acts by engaging the heterodimeric receptor complex consisting of primary receptor IL-22R1 and accessory receptor IL-10R2. IL-22R1 also binds IL-20 and IL-24; IL-10R2 also binds IL-10, IL-27, IL-28, and IL-29. Binding of IL-22 to its receptor complex induces signal transduction, particularly via the JAK-STAT pathway. In addition to the membrane-bound IL-22R1/IL-10R2 complex, a soluble single-chain IL-22 receptor termed IL-22BP has been found to antagonize IL-22 binding and signaling. IL-22 appears not to directly influence immune cells, and major targets of the cytokine appear to be nonimmune cells, such as cells of the skin, digestive and respiratory system, as well as hepatocytes, and keratinocytes. IL-22 has been described as an effector cytokine of the Th17 lineage. Along with IL-17A and IL-17F, IL-22 regulates genes associated with innate immunity of the skin. IL-17A, IL-17F and IL-22 are all co-expressed by Th17 cells, however, they are differentially regulated. The effects of IL-22 include induction of acute phase reactants and antimicrobial proteins, as well as increasing the mobility of keratinocytes. IL-22 is highly expressed during chronic inflammation, and found to activate intracellular kinases and transcription factors. IL-22 is critical for host defense against infections of extracellular pathogens, and promotes wound-healing responses. IL-22 is upregulated in activated T cells. IL-22 has been reported to mediate IL-23-induced acanthosis and dermal inflammation through activation of STAT3.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Identification of Keratinocyte Mitogens: Implications for Hyperproliferation in Psoriasis and Atopic Dermatitis.

JID innovations : skin science from molecules to population health

Niehues H,Rikken G,van Vlijmen-Willems IMJJ,Rodijk-Olthuis D,van Erp PEJ,Zeeuwen PLJM,Schalkwijk J,van den Bogaard EH

200-22 was used in In vitro experiments to investigate the mitogens involved in keratinocyte proliferation and stratification.

Sat Jan 01 00:00:00 EST 2022

MiR-193b-3p-ERBB4 axis regulates psoriasis pathogenesis via modulating cellular proliferation and inflammatory-mediator production of keratinocytes.

Cell death & disease

Huang C,Zhong W,Ren X,Huang X,Li Z,Chen C,Jiang B,Chen Z,Jian X,Yang L,Liu X,Huang H,Shen C,Chen X,Dou X,Yu B

Tue Oct 19 00:00:00 EDT 2021

RPL22 Overexpression Promotes Psoriasis-Like Lesion by Inducing Keratinocytes Abnormal Biological Behavior.

Frontiers in immunology

Zeng J,Zhang Y,Zhang H,Zhang Y,Gao L,Tong X,Xie Y,Hu Q,Chen C,Ding S,Lu J

200-22 was used in Sample Preparation to study the specific mechanism leading to the abnormal biological behavior of keratinocytes and suggest that RPL22 regulates keratinocytes abnormal biological behavior and contributes to the development of psoriatic phenotypes.

Mon Dec 13 00:00:00 EST 2021

The Global Phosphorylation Landscape of SARS-CoV-2 Infection.

Cell

Bouhaddou M,Memon D,Meyer B,White KM,Rezelj VV,Correa Marrero M,Polacco BJ,Melnyk JE,Ulferts S,Kaake RM,Batra J,Richards AL,Stevenson E,Gordon DE,Rojc A,Obernier K,Fabius JM,Soucheray M,Miorin L,Moreno E,Koh C,Tran QD,Hardy A,Robinot R,Vallet T,Nilsson-Payant BE,Hernandez-Armenta C,Dunham A,Weigang S,Knerr J,Modak M,Quintero D,Zhou Y,Dugourd A,Valdeolivas A,Patil T,Li Q,Hüttenhain R,Cakir M,Muralidharan M,Kim M,Jang G,Tutuncuoglu B,Hiatt J,Guo JZ,Xu J,Bouhaddou S,Mathy CJP,Gaulton A,Manners EJ,Félix E,Shi Y,Goff M,Lim JK,McBride T,O'Neal MC,Cai Y,Chang JCJ,Broadhurst DJ,Klippsten S,De Wit E,Leach AR,Kortemme T,Shoichet B,Ott M,Saez-Rodriguez J,tenOever BR,Mullins RD,Fischer ER,Kochs G,Grosse R,García-Sastre A,Vignuzzi M,Johnson JR,Shokat KM,Swaney DL,Beltrao P,Krogan NJ

Thu Aug 06 00:00:00 EDT 2020

The Global Phosphorylation Landscape of SARS-CoV-2 Infection.

Cell

Thu Aug 06 00:00:00 EDT 2020

Challenges and Pitfalls in the Engineering of Human Interleukin 22 (hIL-22) Secreting Lactobacillus reuteri.

Frontiers in bioengineering and biotechnology

Ortiz-Velez L,Goodwin A,Schaefer L,Britton RA

200-22 was used in Western Blotting to investigate development of engineered microbes to treat inflammatory bowel disease and intestinal graft disease.

Mon Sep 28 00:00:00 EDT 2020

EZH2-dependent epigenetic modulation of histone H3 lysine-27 contributes to psoriasis by promoting keratinocyte proliferation.

Cell death & disease

Zhang T,Yang L,Ke Y,Lei J,Shen S,Shao S,Zhang C,Zhu Z,Dang E,Wang G

Sat Oct 03 00:00:00 EDT 2020

Intra- and Inter-cellular Rewiring of the Human Colon during Ulcerative Colitis.

Cell

Smillie CS,Biton M,Ordovas-Montanes J,Sullivan KM,Burgin G,Graham DB,Herbst RH,Rogel N,Slyper M,Waldman J,Sud M,Andrews E,Velonias G,Haber AL,Jagadeesh K,Vickovic S,Yao J,Stevens C,Dionne D,Nguyen LT,Villani AC,Hofree M,Creasey EA,Huang H,Rozenblatt-Rosen O,Garber JJ,Khalili H,Desch AN,Daly MJ,Ananthakrishnan AN,Shalek AK,Xavier RJ,Regev A

Thu Jul 25 00:00:00 EDT 2019

STAT3 and STAT5 Signaling Thresholds Determine Distinct Regulation for Innate Receptor-Induced Inflammatory Cytokines, and STAT3/STAT5 Disease Variants Modulate These Outcomes.

Journal of immunology (Baltimore, Md. : 1950)

Hedl M,Sun R,Huang C,Abraham C

Sun Dec 15 00:00:00 EST 2019

IL-22 and IL-22-Binding Protein Are Associated With Development of and Mortality From Acute-on-Chronic Liver Failure.

Hepatology communications

Schwarzkopf K,Rüschenbaum S,Barat S,Cai C,Mücke MM,Fitting D,Weigert A,Brüne B,Zeuzem S,Welsch C,Lange CM

Fri Mar 01 00:00:00 EST 2019

ATG16L1 orchestrates interleukin-22 signaling in the intestinal epithelium via cGAS-STING.

The Journal of experimental medicine

Aden K,Tran F,Ito G,Sheibani-Tezerji R,Lipinski S,Kuiper JW,Tschurtschenthaler M,Saveljeva S,Bhattacharyya J,Häsler R,Bartsch K,Luzius A,Jentzsch M,Falk-Paulsen M,Stengel ST,Welz L,Schwarzer R,Rabe B,Barchet W,Krautwald S,Hartmann G,Pasparakis M,Blumberg RS,Schreiber S,Kaser A,Rosenstiel P

Mon Nov 05 00:00:00 EST 2018

MicroRNA-17-92 cluster promotes the proliferation and the chemokine production of keratinocytes: implication for the pathogenesis of psoriasis.

Cell death & disease

Zhang W,Yi X,An Y,Guo S,Li S,Song P,Chang Y,Zhang S,Gao T,Wang G,Li C

Tue May 01 00:00:00 EDT 2018

Interleukin-2 induces the in vitro maturation of human pluripotent stem cell-derived intestinal organoids.

Nature communications

Jung KB,Lee H,Son YS,Lee MO,Kim YD,Oh SJ,Kwon O,Cho S,Cho HS,Kim DS,Oh JH,Zilbauer M,Min JK,Jung CR,Kim J,Son MY

Thu Aug 02 00:00:00 EDT 2018

C10orf99 contributes to the development of psoriasis by promoting the proliferation of keratinocytes.

Scientific reports

Chen C,Wu N,Duan Q,Yang H,Wang X,Yang P,Zhang M,Liu J,Liu Z,Shao Y,Zheng Y

Tue Jun 05 00:00:00 EDT 2018

The Therapeutic Potential and Molecular Mechanism of Isoflavone Extract against Psoriasis.

Scientific reports

Li HJ,Wu NL,Lee GA,Hung CF

Fri Apr 20 00:00:00 EDT 2018

A biallelic mutation in IL6ST encoding the GP130 co-receptor causes immunodeficiency and craniosynostosis.

The Journal of experimental medicine

Schwerd T,Twigg SRF,Aschenbrenner D,Manrique S,Miller KA,Taylor IB,Capitani M,McGowan SJ,Sweeney E,Weber A,Chen L,Bowness P,Riordan A,Cant A,Freeman AF,Milner JD,Holland SM,Frede N,Müller M,Schmidt-Arras D,Grimbacher B,Wall SA,Jones EY,Wilkie AOM,Uhlig HH

Mon Sep 04 00:00:00 EDT 2017

GATA3 regulates FLG and FLG2 expression in human primary keratinocytes.

Scientific reports

Zeitvogel J,Jokmin N,Rieker S,Klug I,Brandenberger C,Werfel T

Tue Sep 19 00:00:00 EDT 2017

Interleukin-22 ameliorated renal injury and fibrosis in diabetic nephropathy through inhibition of NLRP3 inflammasome activation.

Cell death & disease

Wang S,Li Y,Fan J,Zhang X,Luan J,Bian Q,Ding T,Wang Y,Wang Z,Song P,Cui D,Mei X,Ju D

Thu Jul 20 00:00:00 EDT 2017

PGE₂ is a direct and robust mediator of anion/fluid secretion by human intestinal epithelial cells.

Scientific reports

Fujii S,Suzuki K,Kawamoto A,Ishibashi F,Nakata T,Murano T,Ito G,Shimizu H,Mizutani T,Oshima S,Tsuchiya K,Nakamura T,Araki A,Ohtsuka K,Okamoto R,Watanabe M

Wed Nov 09 00:00:00 EST 2016

Inhibition of TYK2 and JAK1 ameliorates imiquimod-induced psoriasis-like dermatitis by inhibiting IL-22 and the IL-23/IL-17 axis.

Journal of immunology (Baltimore, Md. : 1950)

Works MG,Yin F,Yin CC,Yiu Y,Shew K,Tran TT,Dunlap N,Lam J,Mitchell T,Reader J,Stein PL,D'Andrea A

Wed Oct 01 00:00:00 EDT 2014

Protein expression profiling of inflammatory mediators in human temporal lobe epilepsy reveals co-activation of multiple chemokines and cytokines.

Journal of neuroinflammation

Kan AA,de Jager W,de Wit M,Heijnen C,van Zuiden M,Ferrier C,van Rijen P,Gosselaar P,Hessel E,van Nieuwenhuizen O,de Graan PN

Thu Aug 30 00:00:00 EDT 2012

Murine insulin growth factor-like (IGFL) and human IGFL1 proteins are induced in inflammatory skin conditions and bind to a novel tumor necrosis factor receptor family member, IGFLR1.

The Journal of biological chemistry

Lobito AA,Ramani SR,Tom I,Bazan JF,Luis E,Fairbrother WJ,Ouyang W,Gonzalez LC

Fri May 27 00:00:00 EDT 2011

Flagellin delivery by Pseudomonas aeruginosa rhamnolipids induces the antimicrobial protein psoriasin in human skin.

PloS one

Meyer-Hoffert U,Zimmermann A,Czapp M,Bartels J,Koblyakova Y,Gläser R,Schröder JM,Gerstel U

Tue Jan 25 00:00:00 EST 2011

Delineation of the innate and adaptive T-cell immune outcome in the human host in response to Campylobacter jejuni infection.

PloS one

Edwards LA,Nistala K,Mills DC,Stephenson HN,Zilbauer M,Wren BW,Dorrell N,Lindley KJ,Wedderburn LR,Bajaj-Elliott M

Tue Nov 09 00:00:00 EST 2010

Multiparametric analysis of cytokine-driven human Th17 differentiation reveals a differential regulation of IL-17 and IL-22 production.

Blood

Volpe E,Touzot M,Servant N,Marloie-Provost MA,Hupé P,Barillot E,Soumelis V

Thu Oct 22 00:00:00 EDT 2009

Innate and adaptive interleukin-22 protects mice from inflammatory bowel disease.

Immunity

Zenewicz LA,Yancopoulos GD,Valenzuela DM,Murphy AJ,Stevens S,Flavell RA

Fri Dec 19 00:00:00 EST 2008

Development and validation of human psoriatic skin equivalents.

The American journal of pathology

Tjabringa G,Bergers M,van Rens D,de Boer R,Lamme E,Schalkwijk J

Mon Sep 01 00:00:00 EDT 2008

Gene expression profiling in autoimmune noninfectious uveitis disease.

Journal of immunology (Baltimore, Md. : 1950)

Li Z,Liu B,Maminishkis A,Mahesh SP,Yeh S,Lew J,Lim WK,Sen HN,Clarke G,Buggage R,Miller SS,Nussenblatt RB

Wed Oct 01 00:00:00 EDT 2008

Inhibition of type I and type III interferons by a secreted glycoprotein from Yaba-like disease virus.

Proceedings of the National Academy of Sciences of the United States of America

Huang J,Smirnov SV,Lewis-Antes A,Balan M,Li W,Tang S,Silke GV,Pütz MM,Smith GL,Kotenko SV

Tue Jun 05 00:00:00 EDT 2007

The interleukin-22/STAT3 pathway potentiates expression of inducible nitric-oxide synthase in human colon carcinoma cells.

The Journal of biological chemistry

Ziesché E,Bachmann M,Kleinert H,Pfeilschifter J,Mühl H

Fri Jun 01 00:00:00 EDT 2007

Bioinformatics

Protein Aliases: Cytokine Zcyto18; il 22; IL-10-related T-cell-derived inducible factor; IL-10-related T-cell-derived-inducible factor; IL-22; IL-TIF; ILN; Interleukin; Interleukin-22; Interleukin22

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Gene Aliases: IL-21; IL-22; IL-D110; IL-TIF; IL22; ILTIF; TIFa; TIFIL-23; UNQ3099/PRO10096; ZCYTO18

Human IL-22 Recombinant Protein, PeproTech®

Human IL-22 Protein (200-22-10UG) in Functional

Product Details

200-22-10UG

Western Blot (WB)

ELISA (ELISA)

Functional Assay (Functional)

In vitro Assay (IV)

Miscellaneous PubMed (Misc)

Species

Published species

Expression System

Amino acid sequence

Molecular weight

Class

Type

Purity

Endotoxin concentration

Activity

Conjugate

Form

Purification

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

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