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Product Details
AF-200-33-10UG
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Published species
Human
Expression System
E. coli
Amino acid sequence
SITGISPITE YLASLSTYND QSITFALEDE SYEIYVEDLK KDEKKDKVLL SYYESQHPSN ESGDGVDGKM LMVTLSPTKD FWLHANNKEH SVELHKCEKP LPDQAFFVLH NMHSNCVSFE CKTDPGVFIG VKDNHLALIK VDSSENLCTE NILFKLSET
Molecular weight
17.9 kDa
Class
Recombinant
Type
Protein
Purity
≥ 98% by SDS-PAGE gel and HPLC analyses.
Endotoxin concentration
<0.1 EU/µg
Activity
Determined by its ability to stimulate the proliferation of mouse D10S cells. The expected ED50 ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 10^7 units/mg.
Conjugate
Form
Lyophilized
Purification
purified
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
Product Specific Information
Recombinant Human IL-33 is a 17.9 kDa protein containing 159 amino acid residues.
This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis
Target Information
IL-33 (Interleukin-33) is a 270 amino acid, highly divergent protein belonging to the IL-1 family with an IL-1-like C-terminal domain. IL-33 is a dual function protein that may function both as a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties. IL-33 binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6. IL-33 activates NF-kappaB and MAP kinases, and drives production of TH2-associated cytokines from in vitro polarized TH2 cells. In vivo, IL-33 induces the expression of IL-4, IL-5, and IL-13 and leads to severe pathological changes in mucosal organs. IL-33 is proteolytically converted to a mature form by CASP1 and is highly expressed in high endothelial venules found in tonsils, Peyer's patches and mesenteric lymph nodes and is almost undetectable in placenta. Prolonged IL-33 treatment of mice led to the development of eosinophilia, splenomegaly, and severe pathological changes in mucosal organs such as lungs, esophagus and small intestine. Recent experiments have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties. Despite its predicted molecular weight, IL-33 will often run at higher molecular weight in SDS-PAGE. Studies have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine, and an intracellular nuclear factor with transcriptional regulatory properties.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DVS27-related protein; growth stimulation-expressed; H-IL-33; homolog of mouse growth stimulation-expressed; IL-1F11; IL-33; ILN; Interleukin; interleukin 1 receptor-like 1; interleukin 1 receptor-related protein; Interleukin-1 family member 11; Interleukin-1 receptor-like 1; Interleukin-33; Interleukin33; NF-HEV; nuclear factor for high endothelial venules; Nuclear factor from high endothelial venules; Protein ST2; RP11-575C20.2
Gene Aliases: C9orf26; DER4; DVS27; FIT-1; IL1F11; IL1RL1; IL33; IL33R; NF-HEV; NFEHEV; NFHEV; ST2; ST2L; ST2V; T1
UniProt ID: (Human) Q01638, (Human) O95760
Entrez Gene ID: (Human) 9173, (Human) 90865
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