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Human IL-33 Recombinant Protein, PeproTech®

Human IL-33 Protein in Functional Assay (Functional)

FIGURE: 1 / 1

Human IL-33 Protein (200-33-10UG) in Functional

Bioassay analysis of Human IL-33 Recombinant Protein, PeproTech® (Product # 200-33-1MG).

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Product Details

200-33-10UG

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

ELISA (ELISA)

Assay-dependent

Functional Assay (Functional)

Assay-dependent

In vitro Assay (IV)

View 22 publications 22 publications

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species

Human

Published species

Human, Mouse

Expression System

E. coli

Amino acid sequence

SITGISPITE YLASLSTYND QSITFALEDE SYEIYVEDLK KDEKKDKVLL SYYESQHPSN ESGDGVDGKM LMVTLSPTKD FWLHANNKEH SVELHKCEKP LPDQAFFVLH NMHSNCVSFE CKTDPGVFIG VKDNHLALIK VDSSENLCTE NILFKLSET

Molecular weight

17.9 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

The ED50 was determined by the dose-dependent stimulation of the proliferation of murine D10S cells is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 10^7 units/mg.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Human IL-33 is a 17.9 kDa protein containing 159 amino acid residues.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

IL-33 (Interleukin-33) is a 270 amino acid, highly divergent protein belonging to the IL-1 family with an IL-1-like C-terminal domain. IL-33 is a dual function protein that may function both as a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties. IL-33 binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6. IL-33 activates NF-kappaB and MAP kinases, and drives production of TH2-associated cytokines from in vitro polarized TH2 cells. In vivo, IL-33 induces the expression of IL-4, IL-5, and IL-13 and leads to severe pathological changes in mucosal organs. IL-33 is proteolytically converted to a mature form by CASP1 and is highly expressed in high endothelial venules found in tonsils, Peyer's patches and mesenteric lymph nodes and is almost undetectable in placenta. Prolonged IL-33 treatment of mice led to the development of eosinophilia, splenomegaly, and severe pathological changes in mucosal organs such as lungs, esophagus and small intestine. Recent experiments have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties. Despite its predicted molecular weight, IL-33 will often run at higher molecular weight in SDS-PAGE. Studies have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine, and an intracellular nuclear factor with transcriptional regulatory properties.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Angiotensin II enhances group 2 innate lymphoid cell responses via AT1a during airway inflammation.

The Journal of experimental medicine

Liu G,Chen Y,Wang Y,Deng X,Xiao Q,Zhang L,Xu H,Han X,Lei A,He J,Li X,Cao Y,Zhou P,He C,Wu P,Jiang W,Tan M,Chen C,Yang Q,Lu L,Deng K,Yao Z,Zhou J

Mon Mar 07 00:00:00 EST 2022

IL-33 Enhances ACE2 Expression on Epidermal Keratinocytes in Atopic Dermatitis: A Plausible Issue for SARS-CoV-2 Transmission in Inflamed Atopic Skin.

Biomedicines

Lin EC,Hong CH

200-33 was used in In vitro experiments to reveal that IL-33 enhances ACE2 expression on keratinocytes via ERK.

Fri May 20 00:00:00 EDT 2022

Differentiation and on axon-guidance chip culture of human pluripotent stem cell-derived peripheral cholinergic neurons for airway neurobiology studies.

Frontiers in pharmacology

Goldsteen PA,Sabogal Guaqueta AM,Mulder PPMFA,Bos IST,Eggens M,Van der Koog L,Soeiro JT,Halayko AJ,Mathwig K,Kistemaker LEM,Verpoorte EMJ,Dolga AM,Gosens R

200-33 was used in In vitro experiments to develop a microfluidic chip, to represent a human in vitro platform, to model neuro-effector interactions in the airways that may be used for mechanistic studies into neuroplasticity in asthma and other lung diseases.

Fri Nov 18 00:00:00 EST 2022

Tissue signals imprint Aiolos expression in ILC2s to modulate type 2 immunity.

Mucosal immunology

Qiu J,Zhang J,Ji Y,Sun H,Gu Z,Sun Q,Bai M,Gong J,Tang J,Zhang Y,Li S,Shao Z,Li J,Sheng H,Shen L,Qiu J

Mon Nov 01 00:00:00 EDT 2021

Oxidation-Dependent Activation of Src Kinase Mediates Epithelial IL-33 Production and Signaling during Acute Airway Allergen Challenge.

Journal of immunology (Baltimore, Md. : 1950)

Dustin CM,Habibovic A,Hristova M,Schiffers C,Morris CR,Lin MJ,Bauer RA,Heppner DE,Daphtary N,Aliyeva M,van der Vliet A

Tue Jun 15 00:00:00 EDT 2021

IL-33 promotes anemia during chronic inflammation by inhibiting differentiation of erythroid progenitors.

The Journal of experimental medicine

Swann JW,Koneva LA,Regan-Komito D,Sansom SN,Powrie F,Griseri T

Mon Sep 07 00:00:00 EDT 2020

Expansion of Group 2 Innate Lymphoid Cells in Patients with End-Stage Renal Disease and Their Clinical Significance.

Journal of immunology (Baltimore, Md. : 1950)

Liu GY,Deng XH,Li X,Cao YJ,Xing YF,Zhou P,Lei AH,Yang Q,Deng K,Zhang H,Zhou J

Wed Jul 01 00:00:00 EDT 2020

Blockade of IL-33 signalling attenuates osteoarthritis.

Clinical & translational immunology

He Z,Song Y,Yi Y,Qiu F,Wang J,Li J,Jin Q,Sacitharan PK

Mon Apr 18 00:00:00 EDT 2022

Genome-wide Analyses of Chromatin State in Human Mast Cells Reveal Molecular Drivers and Mediators of Allergic and Inflammatory Diseases.

Immunity

Cildir G,Toubia J,Yip KH,Zhou M,Pant H,Hissaria P,Zhang J,Hong W,Robinson N,Grimbaldeston MA,Lopez AF,Tergaonkar V

Tue Nov 19 00:00:00 EST 2019

Endothelial Sox17 promotes allergic airway inflammation.

The Journal of allergy and clinical immunology

Ha EH,Choi JP,Kwon HS,Park HJ,Lah SJ,Moon KA,Lee SH,Kim I,Cho YS

200-33 was used in In vitro experiments to investigate the relationship between Sox17 and IL-33 and the possible role of Sox17 in the pathogenesis of asthma using a mouse model of airway inflammation.

Thu Aug 01 00:00:00 EDT 2019

The interleukin-33-mediated inhibition of expression of two key genes implicated in atherosclerosis in human macrophages requires MAP kinase, phosphoinositide 3-kinase and nuclear factor-κB signaling pathways.

Scientific reports

Buckley ML,Williams JO,Chan YH,Laubertová L,Gallagher H,Moss JWE,Ramji DP

Mon Aug 05 00:00:00 EDT 2019

Genetic programming of macrophages generates an in vitro model for the human erythroid island niche.

Nature communications

Lopez-Yrigoyen M,Yang CT,Fidanza A,Cassetta L,Taylor AH,McCahill A,Sellink E,von Lindern M,van den Akker E,Mountford JC,Pollard JW,Forrester LM

200-33 was used in Cell Culture to develop an in vitro system to model the human erythroblastic island niche.

Wed Feb 20 00:00:00 EST 2019

Differential Modulation of Human Innate Lymphoid Cell (ILC) Subsets by IL-10 and TGF-β.

Scientific reports

Bonne-Année S,Bush MC,Nutman TB

Fri Oct 04 00:00:00 EDT 2019

Resveratrol inhibits IL-33-mediated mast cell activation by targeting the MK2/3-PI3K/Akt axis.

Scientific reports

Nakajima S,Ishimaru K,Kobayashi A,Yu G,Nakamura Y,Oh-Oka K,Suzuki-Inoue K,Kono K,Nakao A

Thu Dec 05 00:00:00 EST 2019

CD4⁺ Regulatory T Cells Exert Differential Functions during Early and Late Stages of the Immune Response to Respiratory Viruses.

Journal of immunology (Baltimore, Md. : 1950)

Rogers MC,Lamens KD,Shafagati N,Johnson M,Oury TD,Joyce S,Williams JV

Wed Aug 15 00:00:00 EDT 2018

IL-33 Upregulates Cysteinyl Leukotriene Receptor Type 1 Expression in Human Peripheral Blood CD4⁺ T Lymphocytes.

Journal of immunology (Baltimore, Md. : 1950)

Boudaud M,Turcotte S,Stankova J,Rola-Pleszczynski M

Thu Nov 01 00:00:00 EDT 2018

Chitin promotes antigen-specific Th2 cell-mediated murine asthma through induction of IL-33-mediated IL-1β production by DCs.

Scientific reports

Arae K,Morita H,Unno H,Motomura K,Toyama S,Okada N,Ohno T,Tamari M,Orimo K,Mishima Y,Suto H,Okumura K,Sudo K,Miyazawa H,Taguchi H,Saito H,Matsumoto K,Nakae S

Mon Aug 06 00:00:00 EDT 2018

Chromatin regulates IL-33 release and extracellular cytokine activity.

Nature communications

Travers J,Rochman M,Miracle CE,Habel JE,Brusilovsky M,Caldwell JM,Rymer JK,Rothenberg ME

Tue Aug 14 00:00:00 EDT 2018

Reversal of B-cell hyperactivation and functional impairment is associated with HBsAg seroconversion in chronic hepatitis B patients.

Cellular & molecular immunology

Xu X,Shang Q,Chen X,Nie W,Zou Z,Huang A,Meng M,Jin L,Xu R,Zhang JY,Fu J,Wang L,Tang Z,Xie Y,Yang X,Zhang Z,Wang FS

Fri May 01 00:00:00 EDT 2015

Human Th2 but not Th9 cells release IL-31 in a STAT6/NF-κB-dependent way.

Journal of immunology (Baltimore, Md. : 1950)

Maier E,Werner D,Duschl A,Bohle B,Horejs-Hoeck J

Tue Jul 15 00:00:00 EDT 2014

The GOLD domain-containing protein TMED1 is involved in interleukin-33 signaling.

The Journal of biological chemistry

Connolly DJ,O'Neill LA,McGettrick AF

Fri Feb 22 00:00:00 EST 2013

mTOR and GSK-3 shape the CD4+ T-cell stimulatory and differentiation capacity of myeloid DCs after exposure to LPS.

Blood

Turnquist HR,Cardinal J,Macedo C,Rosborough BR,Sumpter TL,Geller DA,Metes D,Thomson AW

Thu Jun 10 00:00:00 EDT 2010

mTOR and GSK-3 shape the CD4+ T-cell stimulatory and differentiation capacity of myeloid DCs after exposure to LPS.

Blood

Turnquist HR,Cardinal J,Macedo C,Rosborough BR,Sumpter TL,Geller DA,Metes D,Thomson AW

Thu Jun 10 00:00:00 EDT 2010

IL-33 mediates inflammatory responses in human lung tissue cells.

Journal of immunology (Baltimore, Md. : 1950)

Yagami A,Orihara K,Morita H,Futamura K,Hashimoto N,Matsumoto K,Saito H,Matsuda A

Mon Nov 15 00:00:00 EST 2010

IL-33 reduces macrophage foam cell formation.

Journal of immunology (Baltimore, Md. : 1950)

McLaren JE,Michael DR,Salter RC,Ashlin TG,Calder CJ,Miller AM,Liew FY,Ramji DP

Thu Jul 15 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: DVS27-related protein; growth stimulation-expressed; H-IL-33; homolog of mouse growth stimulation-expressed; IL-1F11; IL-33; ILN; Interleukin; interleukin 1 receptor-like 1; interleukin 1 receptor-related protein; Interleukin-1 family member 11; Interleukin-1 receptor-like 1; Interleukin-33; Interleukin33; NF-HEV; nuclear factor for high endothelial venules; Nuclear factor from high endothelial venules; Protein ST2; RP11-575C20.2

View more View less

Gene Aliases: C9orf26; DER4; DVS27; FIT-1; IL1F11; IL1RL1; IL33; IL33R; NF-HEV; NFEHEV; NFHEV; ST2; ST2L; ST2V; T1

Human IL-33 Recombinant Protein, PeproTech®

Human IL-33 Protein (200-33-10UG) in Functional

Product Details

200-33-10UG

Western Blot (WB)

ELISA (ELISA)

Functional Assay (Functional)

In vitro Assay (IV)

Miscellaneous PubMed (Misc)

Species

Published species

Expression System

Amino acid sequence

Molecular weight

Class

Type

Purity

Endotoxin concentration

Activity

Conjugate

Form

Purification

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

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