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Product Details
RIL4I
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Published species
Not Applicable
Expression System
P. pastoris
Amino acid sequence
Human IL-4, amino acids 25-153 (Accession # P05112)
Molecular weight
15 kDa
Class
Recombinant
Type
Protein
Purity
>95% by SDS-PAGE
Activity
Determined by the proliferation of human TF-1 cells.
Conjugate
Form
Liquid
Purification
purified
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Mature human IL-4 was expressed in Pichia pastoris, X-33. GenBank Accession # P05112, amino acids 25 - 153. For protein verification, the expressed protein was N-terminal sequenced and begins with the following 10 amino acids: HKCDITLQEI. Purity is >95% by SDS-PAGE analyses.
Activity: The biological activity of recombinant human IL-4 was determined by measuring the proliferation of the factor-dependent human T-helper cell line, TF-1.
Use: For in vitro applications. A dose-response assay should be performed to determine the optimal concentration for specific experiments.
Target Information
Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: B cell growth factor 1; B-cell stimulatory factor 1; B_cell stimulatory factor 1; Binetrakin; BSF-1; H-IL-4; IL-4; ILN; Interleukin; interleukin 4 variant 2; Interleukin-4; Interleukin4; Lymphocyte stimulatory factor 1; M-IL-4; MGC79402; Pitrakinra
Gene Aliases: BCGF-1; BCGF1; BSF-1; BSF1; IL-4; IL4
UniProt ID: (Human) P05112
Entrez Gene ID: (Human) 3565
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