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Product Details
PHC0055
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
Disulfide-linked homodimer with each subunit containing 116 amino acid residues
Molecular weight
26 kDa
Class
Recombinant
Type
Protein
Purity
≥98% by SDS-PAGE and HPLC
Endotoxin concentration
<0.1 ng/µg
Activity
ED50 ≤ 0.15 ng/mL; determined by the dose-dependent proliferation of human TF-1 cells.
Conjugate
Form
Lyophilized
Purification
purified
Storage buffer
20mM sodium phosphate, pH 7.5
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Wet ice (domestic); Wet or Dry ice (international)
Product Specific Information
Carrier-Free
Reconstitution: We recommend that the vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute recombinant human IL-5 in sterile distilled water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2°C to 8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein, such as 0.1% BSA and store in working aliquots at -20°C to 280°C.
Storage: The lyophilized protein is stable at room temperature for 1 month, but should be kept at -20°C for long term storage. Working aliquots stored with a carrier protein are stable for at least 12 months at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
Target Information
IL-5 is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. IL-5 cytokine is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes. The receptor of IL-5 cytokine is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). IL-5, together with those for interleukin 4 (IL4), interleukin 13 (IL13), and CSF2, form a cytokine gene cluster on chromosome 5. IL-5, IL4, and IL13 are found to be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B-cell differentiation factor I; colony-stimulating factor, eosinophil; Eosinophil differentiation factor; IL-5; ILN; Interleukin; Interleukin-5; Interleukin5; T-cell replacing factor; TRF
Gene Aliases: EDF; IL-5; IL5; TRF
UniProt ID: (Human) P05113
Entrez Gene ID: (Human) 3567
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