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Invitrogen
Human JMJD2B (aa 1036-1090) Control Fragment Recombinant Protein
Product Details
RP-107204
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
SRLSLSTGAPQEPAFSGEEAKAAKRPRVGTPLATEDSGRSQDYVAFVESLLQVQG
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
1M urea/PBS, pH 7.4
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (70%), Rat (70%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibodies, PA5-66540, PA5-145006. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27', H3 'Lys-36' nor H4 'Lys-20'. Only able to demethylate trimethylated H3 'Lys-9', with a weaker activity than KDM4A, KDM4C and KDM4D. Demethylation of Lys residue generates formaldehyde and succinate. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: [histone H3]-trimethyl-L-lysine(9) demethylase 4B; JmjC domain-containing histone demethylation protein 3B; jumonji domain containing 2B; Jumonji domain-containing protein 2B; lysine (K)-specific demethylase 4B; Lysine-specific demethylase 4B; tudor domain containing 14B
Gene Aliases: JHDM3B; JMJD2B; KDM4B; KIAA0876; TDRD14B
UniProt ID: (Human) O94953
Entrez Gene ID: (Human) 23030
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